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Analysis of the complete genome sequence of black queen cell virus JL1 from infected honeybees in China

Published online by Cambridge University Press:  05 July 2016

Q. Yang ,
Z.-Y. Song ,
X. Feng ,
J. Zhang ,
Y. Zheng ,
X.-H. Wang ,
J.-C. Sui ,
Z.-G. Wang  and
Y. Sun
Q. Yang
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
Z.-Y. Song
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
X. Feng*
Affiliation:
College Veterinary Medicine Jilin University, Changchun 130062, China
J. Zhang
Affiliation:
Changchun Institute of Biological Products Co., Ltd., Changchun 130062, China
Y. Zheng
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
X.-H. Wang
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
J.-C. Sui
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
Z.-G. Wang
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
Y. Sun
Affiliation:
JiLin Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Changchun 130062, China
*
*Author for correspondence Phone: 86-431-87607322 Fax: 86-431-87607989 E-mail: [email protected]
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Abstract

There are six strains of the complete genomic sequences of black queen cell virus (BQCV) published in the GenBank, including South Africa (AF183905), South Korea (JX149531), Hungary 10 (EF517515), Poland 4 (EF517519), Poland 5 (EF517520) and Poland 6 (EF517521). Based on the six BQCV strains published in the GenBank, ten pairs of primers were designed in the present study using reverse transcription polymerase chain reaction to obtain the first complete genome sequence of a BQCV strain in China, called the BQCV China-JL1 strain (KP119603). A phylogenetic tree was then built to analyse their genetic relationships. The BQCV China-JL1 strain showed 86–93% similarity with the six strains published in the GenBank. The BQCV China-JL1 strain consisted of 8358 nucleotides (nt). The 5′-proximal open reading frame (ORF1) initiated at nt position 546 and terminated at nt position 4676, ORF3 initiated at nt position 4891 and terminated at nt position 5433, and the 3′-proximal ORF (ORF2) was located between nt positions 5750 and 8203.

Keywords

BQCVcomplete genome sequenceRT–PCRphylogenetic analysisRdRp
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 5 , October 2016 , pp. 561 - 568
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Copyright
Copyright © Cambridge University Press 2016 

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