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An integrated diagnostic setup for the morphological and molecular identification of the Ceratitis FAR complex (C. anonae, C. fasciventris, C. rosa, C. quilicii, Diptera, Tephritidae)

Published online by Cambridge University Press:  11 September 2018

M. Virgilio Open the ORCID record for M. Virgilio [Opens in a new window] ,
J.-H. Daneel ,
A. Manrakhan ,
H. Delatte Open the ORCID record for H. Delatte [Opens in a new window] ,
K. Meganck  and
M. De Meyer
M. Virgilio*
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
J.-H. Daneel
Affiliation:
Citrus Research International, P.O Box 28, Nelspruit 1200, South Africa
A. Manrakhan
Affiliation:
Citrus Research International, P.O Box 28, Nelspruit 1200, South Africa
H. Delatte
Affiliation:
CIRAD-UMR PVBMT, CIRAD-3P, Saint Pierre, France
K. Meganck
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
M. De Meyer
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
*
*Author for correspondence Phone: +32 (0) 27695854 E-mail: [email protected]
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Abstract

The Ceratitis FAR complex (Diptera, Tephritidae) includes four economically important frugivorous flies (Ceratitis anonae, Ceratitis fasciventris, Ceratitis quilicii, Ceratitis rosa) whose immature stages and adult females cannot be properly resolved through morphological identification. In order to develop a simplified molecular tool for the identification of two of these species (C. rosa, C. quilicii), we selected a subset of six microsatellite markers out of a panel of 16 loci that were previously developed for the molecular differentiation of the taxa within the complex. These six markers were first tested in silico and then used for the actual genotyping of C. quilicii and C. rosa, resulting in the correct identification of all male reference specimens. Here, we propose an integrated morphological and molecular setup for the identification of the four species of the FAR complex. The decision map relies on preliminary DNA barcoding or morphological identification (when possible) to exclude species not belonging to the complex followed by (a) morphological identification of all adult male specimens and female C. anonae, (b) molecular identification via a panel of 16 microsatellite markers for immature stages, damaged vouchers and samples potentially including adult female C. fasciventris/C. quilicii/C. rosa and (c) molecular identification via a reduced panel of six microsatellite markers for samples including only C. quilicii and C. rosa. This simplified diagnostic setup was profitably implemented in the framework of the ERAfrica fruit fly project and will help correctly identify species within the FAR complex for their early detection and monitoring.

Keywords

molecular identificationDNA barcodingmicrosatellitesC. quiliciiC. rosaFAR complexdecision map
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 3 , June 2019 , pp. 376 - 382
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Copyright
Copyright © Cambridge University Press 2018 

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