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Allozyme markers to help define the South American origins of Microctonus hyperodae (Hymenoptera: Braconidae) established in New Zealand for biological control of Argentine stem weevil

Published online by Cambridge University Press:  09 March 2007

I.I. Iline  and
C.B. Phillips
I.I. Iline*
Affiliation:
New Zealand Pastoral Agriculture Research Institute Ltd, Canterbury Agriculture and Science Centre, PO Box 60, Lincoln, New Zealand
C.B. Phillips
Affiliation:
New Zealand Pastoral Agriculture Research Institute Ltd, Canterbury Agriculture and Science Centre, PO Box 60, Lincoln, New Zealand
*
*Fax: + 64-3-983 3904. E-mail: [email protected]
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Abstract

The thelytokous parasitoid, Microctonus hyperodae Loan, was collected from eight South American locations and introduced to New Zealand in 1991 for biological control of Argentine stem weevil, Listronotus bonariensis (Kuschel) (Coleoptera: Curculionidae). Parasitoids from each population were released in equal numbers at each New Zealand site to give them the same opportunities to establish. Population markers have been sought to identify the South American geographic populations that have become most successful in New Zealand. These would assist in determining the importance of concepts such as climate matching and host–parasitoid coevolution to the establishment of natural enemies in new regions for biological control. Vertical polyacrylamide electrophoresis was used to survey 16 enzymes and ten calcium binding proteins, and this paper reports variation at three putative loci. Malate dehydrogenase, a dihydrolipoamide dehydrogenase isozyme and a calcium binding protein exhibited clear genetic variation, each with two alleles. All M. hyperodae isofemale lines from east of the Andes mountains shared one genotype, all but one from west of the Andes shared another, while a population from within the Andes contained both genotypes. This variation was highly congruent with previously described morphometric variation. At two loci, the maintenance of heterozygotes, and the absence of homozygotes, within isofemale lines suggested M. hyperodae thelytoky is apomictic.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 94 , Issue 3 , June 2004 , pp. 229 - 234
Copyright
Copyright © Cambridge University Press 2004

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References

Coelho, J.R. & Mitton, J.B. (1988) Oxygen consumption during hovering is associated with genetic variation of enzymes in honey-bees. Functional Ecology 2, 141–146.CrossRefGoogle Scholar
Cornuet, J.M., Oldroyd, B.P. & Crozier, R.H. (1995) Unequal thermostability of allelic forms of malate dehydrogenase in honey bees. Journal of Apicultural Research 34, 45–47.CrossRefGoogle Scholar
Fu, J., MacCulloch, R.D., Murphy, R.W., Darevsky, I.S. & Tuniyev, B.S. (2000) Allozyme variation patterns and multiple hybridization origins: clonal variation among four sibling parthenogenetic Caucasian rock lizards. Genetica 108, 107–112.CrossRefGoogle ScholarPubMed
Goldson, S.L.McNeill, M.R., Stufkens, M.W., Proffitt, J.R. & Farrell, J.A. (1990) Importation and quarantine of Microctonus hyperodae, a South American parasitoid of Argentine stem weevil. New Zealand Plant Protection 43, 334–338.Google Scholar
Goldson, S.L., Barker, G.M. & Barratt, B.I.P. (1994) The establishment of the Argentine stem weevil parasitoid at its release sites. New Zealand Plant Protection 47, 274–276.CrossRefGoogle Scholar
Goldson, S.L., Proffitt, J.R. & Baird, D.B. (1998) Establishment and phenology of the parasitoid Microctonus hyperodae (Hymenoptera: Braconidae) in New Zealand. Environmental Entomology 27, 1386–1392.CrossRefGoogle Scholar
Graur, D. (1985) Gene diversity in Hymenoptera. Evolution 39, 190–199.CrossRefGoogle ScholarPubMed
Gu, H.N. (1991) Electrophoretic variation at flight-related enzyme loci and its possible association with flight capacity in Epiphyas postvittana. Biochemical Genetics 29, 345–354.CrossRefGoogle ScholarPubMed
Harrison, J.F., Nielsen, D.I. & Page, R.E.J. (1996) Malate dehydrogenase phenotype, temperature and colony effects on flight metabolic rate in the honey-bee, Apis mellifera. Functional Ecology 10, 81–88.CrossRefGoogle Scholar
Hedrick, P.W. & Parker, J.D. (1997) Evolutionary genetics and genetic variation of haplodiploids and x-linked genes. Annual Review of Ecology and Systematics 28, 55–83.CrossRefGoogle Scholar
Hepburn, H.R., Radloff, S.E. & Fuchs, S. (1999) Flight machinery dimensions of honeybees, Apis mellifera. Journal of Comparative Physiology B 169, 107–112.CrossRefGoogle Scholar
Iline, I.I. & Phillips, C.B. (2003) Allozyme variation between European and New Zealand populations of Microctonus aethiopoides. New Zealand Plant Protection 56 133–137.CrossRefGoogle Scholar
Iline, I.I., Phillips, C.B., Goldson, S.L. & Chapman, H.M. (2002) Allozyme variation between South American geographical populations of Microctonus hyperodae. New Zealand Plant Protection 55, 267–271.CrossRefGoogle Scholar
Lenney, Williams C., Goldson, S.L., Baird, D.B. & Bullock, D.W. (1994) Geographical origin of an introduced insect pest, Listronotus bonariensis (Kuschel), determined by RAPD analysis. Heredity 72, 412–419.Google Scholar
Lester, L.J. & Selander, R.K. (1979) Population genetics of haplodiploid insects. Genetics 92, 1329–1343.CrossRefGoogle ScholarPubMed
Loan, C.C. & Lloyd, D.C. (1974) Description and field biology of Microctonus hyperodae Loan n. sp. (Hymenoptera: Braconidae, Euphorinae) a parasite of Hyperodes bonariensis in South America (Coleoptera: Curculionidae). Entomophaga 19, 7–12.CrossRefGoogle Scholar
Manchenko, G.P. (1994) Handbook of detection of enzymes on electrophoretic gels. Boca Raton, Florida, CRC Press.Google Scholar
McNeill, M.R., Goldson, S.L., Proffitt, J.R., Phillips, C.B. & Addison, P.J. (2002) A description of the commercial rearing and distribution of Microctonus hyperodae (Hymenoptera: Braconidae) for biological control of Listronotus bonariensis (Kuschel) (Coleoptera: Curculionidae). Biological Control 24, 165–175.CrossRefGoogle Scholar
Metcalf, R.A., Marlin, J.C. & Whitt, G.S. (1975) Low levels of genetic heterozygosity in Hymenoptera. Nature 257, 792–794.CrossRefGoogle ScholarPubMed
Murphy, R.W., Sites, J.W., Buth, D.G. & Haufler, C.H. (1996) Proteins: isozyme electrophoresis. pp. 51–120in Hillis, D.M., Moritz, C. & Mable, B.K. (Eds) Molecular systematics. 2nd edn. Sunderland, Massachusetts, Sinauer Associates.Google Scholar
Nielsen, D., Page, R.E.J. & Crosland, M.W.J. (1994) Clinal variation and selection of MDH allozymes in honey bee populations. Experientia 50, 867–871.CrossRefGoogle ScholarPubMed
Owen, R.E. (1993) Genetics of parasitic Hymenoptera. pp. 69–89in Narang, S.K., Bartlett, A.C. & Faust, R.M. (Eds) Applications of genetics to arthropods of biological control significance. Boca Raton, Florida, CRC. Press.Google Scholar
Packer, L. & Owen, R.E. (1992) Variable enzyme systems in the Hymenoptera. Biochemical Systematics and Ecology 20, 1–7.CrossRefGoogle Scholar
Pamilo, P., Varvio-Aho, S. & Pekkarinen, A. (1978) Low enzyme gene variability in Hymenoptera as a consequence of haplodiploidy. Hereditas 88, 93–99.CrossRefGoogle Scholar
Phillips, C.B. (1996) Intraspecific variation in Microctonus hyperodae and M. aethiopoides (Hymenoptera: Braconidae); significance for their use as biological control agents. 167 pp. Unpublished PhD thesis, New Zealand, Lincoln University.Google Scholar
Phillips, C.B. & Baird, D.B. (1996) A morphometric method to assist in defining the South American origins of Microctonus hyperodae Loan (Hymenoptera: Braconidae) established in New Zealand. Biocontrol Science and Technology 6, 189–205.CrossRefGoogle Scholar
Phillips, C.B. & Baird, D.B. (2001) Geographic variation in egg load of Microctonus hyperodae Loan (Hymenoptera: Braconidae) and its implication for biological control success. Biocontrol Science and Technology 11, 371–380.CrossRefGoogle Scholar
Phillips, C.B., Baird, D.B. & Goldson, S.L. (1997) South American origins of Microctonus hyperodae (Hymenoptera: Braconidae) established in New Zealand as defined by morphometric analysis. Biocontrol Science and Technology 7, 247–258.CrossRefGoogle Scholar
Phillips, C.B.McNeil, M.R., Cane, R.P. & Goldson, S.L. (2000) Progress in defining the significance of intraspecific variation to the success of the Argentine stem weevil parasitoid in New Zealand. New Zealand Plant Protection 53, 425–429.CrossRefGoogle Scholar
Prestidge, R.A., Barker, G.M. & Pottinger, R.P. (1991) The economic cost of Argentine stem weevil in pastures in New Zealand. pp. 165–170in Proceeding of the 44th New Zealand Weed and Pest Control Conference.CrossRefGoogle Scholar
Rossler, Y., DeBach, P. (1973) Genetic variability in a thelytokous form of Aphytis mytilaspidis (Le Baron) (Hymenoptera: Aphelinidae). Hilgardia 42, 149–175.CrossRefGoogle Scholar
Rothe, G.M. (1994) Electrophoresis of enzymes: laboratory methods. New York, Springer-Verlag.CrossRefGoogle Scholar
Simon, J.-C., Delmotte, F., Rispe, C. & Crease, T. (2003) Phylogenetic relationships between parthenogens and their sexual relatives: the possible routes to parthenogenesis in animals. Biological Journal of the Linnean Society 79, 151–163.CrossRefGoogle Scholar
Torgerson, D., Lampo, M. & Woo, P.T.K. (2001) Ability of cellulose acetate and acrylamide enzyme electrophoresis to separate 13 species of phlebotomine sand flies (Diptera: Psychodidae) from Venezuela. Journal of Medical Entomology 38, 501–509.CrossRefGoogle ScholarPubMed
Werren, J.H. (1997) Biology of Wolbachia. Annual Review of Entomology 42, 587–609.CrossRefGoogle ScholarPubMed
Wilson, A.C.C., Sunnucks, P. & Hales, D.F. (2003) Heritable genetic variation and potential for adaptive evolution in asexual aphids (Aphidoidea). Biological Journal of the Linnean Society 79, 115–135.CrossRefGoogle Scholar