Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-25T18:17:00.204Z Has data issue: false hasContentIssue false
  • English
  • Français

Variations in the amino acids of haemolymph protein in females of Glossina austeni Newst. (Diptera, Glossinidae) during the reproductive cycle

Published online by Cambridge University Press:  10 July 2009

S. S. Tobe ,
P. A. Langley  and
D. L. Whitehead
S. S. Tobe
Affiliation:
Agricultural Research Council, Unit of Invertebrate Chemistry and Physiology, University of Sussex, Falmer, Brighton, Sussex BN1 9QJ, England
P. A. Langley
Affiliation:
Tsetse Research Laboratory, University of Bristol, School of Veterinary Science, Langford, Bristol BS18 7DU, England
D. L. Whitehead
Affiliation:
Tsetse Research Laboratory, University of Bristol, School of Veterinary Science, Langford, Bristol BS18 7DU, England
Get access Rights & Permissions [Opens in a new window]

Abstract

The amino acids of the haemolymph proteins of female Glossina austeni Newst. have been analysed at selected times during the first two pregnancy cycles by automatic amino acid analysis. The most prominent amino acids were glutamate, aspartate, lysine and leucine, with these amino acids accounting for almost 50% of the total amino acid residues. The molar percentage of all 16 amino acids observed varied only slightly throughout the pregnancy cycles. The amino acids of the perchloric acid-precipitable fraction of the gut contents of third-instar larvae (considered to be equivalent to milk secretion) have also been analysed and differ considerably from the haemolymph proteins, with glutamate and phenylalanine particularly prominent. Because of their low content of aromatic amino acids, the haemolymph proteins are not regarded as an important store of these compounds.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 65 , Issue 2 , June 1975 , pp. 279 - 284
Copyright
Copyright © Cambridge University Press 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Balogun, R. A. (1969). Amino acids in pupa of tsetse fly (Glossina palpalis).—Comp. Bio-chem. Physiol. 30, 785–787.CrossRefGoogle ScholarPubMed
Balogun, R. A. (1971). Studies on the protein metabolism of Glossina palpalis: quantitative differences in free amino acids between male and female adult tsetse flies.—Comp. Biochem. Physiol. 38B, 347–352.Google Scholar
Balogun, R. A., Hanimann, F. & Chen, P. S. (1969). Separation of free amino acids and derivatives in the tsetse fly Glossina palpalis (Diptera) by ion-exchange chromatography.—Experientia 25, 93–95.CrossRefGoogle ScholarPubMed
Bodnaryk, R. P. (1972). A survey of the occurrence of β-alanyl-tyrosine, δ-glutamyl-phenyl-alanine and tyrosine-o-phosphate in the larval stage of flies (Diptera).—Comp. Biochem. Physiol. 43B, 587–592.Google Scholar
Bursell, E. (1960). Free amino-acids of the tsetse fly (Glossina).—Nature, Lond. 187, 778.CrossRefGoogle ScholarPubMed
Bursell, E. (1963). Aspects of the metabolism of amino acids in the tsetse fly, Glossina (Diptera).—J. Insect Physiol. 9, 439–452.CrossRefGoogle Scholar
Cmelik, S. H. W., Bursell, E. & Slack, E. (1969). Composition of the gut contents of third-instar tsetse larvae (Glossina morsitans Westwood).—Comp. Biochem. Physiol. 29, 447–453.CrossRefGoogle Scholar
Cunningham, I. & Slater, J. S. (1974). Amino acid analyses of haemolymph of Glossina morsitans morsitans (Westwood).—Acta trop. 31, 83–88.Google ScholarPubMed
Munn, E. A. & Greville, G. D. (1969). The soluble proteins of developing Calliphora erythrocephala, particularly calliphorin, and similar proteins in other insects.—J. Insect Physiol. 15, 1935–1950.CrossRefGoogle Scholar
Munn, E. A., Feinstein, A. & Greville, G. D. (1971). The isolation and properties of the protein calliphorin.—Biochem. J. 124, 367–374.CrossRefGoogle ScholarPubMed
Nash, T. A. M., Jordan, A. M. & Boyle, J. A. (1968). The large-scale rearing of Glossina austeni Newst. in the laboratory. IV.—The final technique.—Ann. trop. Med. Para-sit. 62, 336–341.CrossRefGoogle ScholarPubMed
Pratt, G. E. (1967). Association of folin-positive material with the blood proteins of the American cockroach and the oviposition cycle.—Nature, Lond. 214, 1034–1035.CrossRefGoogle Scholar
Tobe, S. S. (1972). Aspects of nutrient transfer during the reproductive cycles of the female of the tsetse fly, Glossina austeni Newst.—Ph.D. Thesis, McGill University.Google Scholar
Tobe, S. S. & Davey, K. G. (1972). Volume relationships during the pregnancy cycle of the tsetse fly Glossina austeni.—Can. J. Zool. 50, 999–1010.CrossRefGoogle ScholarPubMed
Tobe, S. S. & Davey, K. G. (in press). Synthesis and turnover of haemolymph proteins during the reproductive cycle of Glossina austeni.—Can. J. Zool.Google Scholar
Tobe, S. S., Davey, K. G. & Huebner, E. (1973). Nutrient transfer during the reproductive cycle in Glossina austeni Newst.: histology and histo-chemistry of the milk gland, fat body, and oenocytes.—Tissue & Cell 5, 633–650.CrossRefGoogle Scholar