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Population differentiation between Australian and Chinese Helicoverpa armigera occurs in distinct blocks on the Z-chromosome

Published online by Cambridge University Press:  05 February 2018

S.V. Song ,
C. Anderson ,
R.T. Good ,
S. Leslie ,
Y. Wu ,
J.G. Oakeshott  and
C. Robin
S.V. Song
Affiliation:
School of Biosciences, University of Melbourne, Victoria, Australia Bio21 Institute, Parkville, Victoria, Australia
C. Anderson
Affiliation:
MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, UK Land and Water Flagship, Commonwealth Scientific and Industrial Research Organisation, Australian Capital Territory, Australia
R.T. Good
Affiliation:
School of Biosciences, University of Melbourne, Victoria, Australia Bio21 Institute, Parkville, Victoria, Australia
S. Leslie
Affiliation:
School of Biosciences, University of Melbourne, Victoria, Australia School of Mathematics and Statistics, University of Melbourne, Victoria, Australia Centre for Systems Genomics, University of Melbourne, Victoria, Australia
Y. Wu
Affiliation:
College of Plant Protection, Nanjing Agricultural University, Nanjing, China
J.G. Oakeshott
Affiliation:
Land and Water Flagship, Commonwealth Scientific and Industrial Research Organisation, Australian Capital Territory, Australia
C. Robin*
Affiliation:
School of Biosciences, University of Melbourne, Victoria, Australia Bio21 Institute, Parkville, Victoria, Australia
*
*Author for correspondence: Tel: +61 3 8344 2349 E-mail: [email protected]
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Abstract

Over the last 40 years, many types of population genetic markers have been used to assess the population structure of the pest moth species Helicoverpa armigera. While this species is highly vagile, there is evidence of inter-continental population structure. Here, we examine Z-chromosome molecular markers within and between Chinese and Australian populations. Using 1352 polymorphic sites from 40 Z-linked loci, we compared two Chinese populations of moths separated by 700 km and found virtually no population structure (n = 41 and n = 54, with <1% of variation discriminating between populations). The levels of nucleotide diversity within these populations were consistent with previous estimates from introns in Z-linked genes of Australian samples (π = 0.028 vs. 0.03). Furthermore, all loci surveyed in these Chinese populations showed a skew toward rare variants, with ten loci having a significant Tajima's D statistic, suggesting that this species could have undergone a population expansion. Eight of the 40 loci had been examined in a previous study of Australian moths, of which six revealed very little inter-continental population structure. However, the two markers associated with the Cyp303a1 locus that has previously been proposed to be a target of a selective sweep, exhibited allele structuring between countries. Using a separate dataset of 19 Australian and four Chinese moths, we scanned the molecular variation distributed across the entire Z-chromosome and found distinct blocks of differentiation that include the region containing Cyp303a1. We recommend some of these loci join those associated with insecticide resistance to form a set of genes best suited to analyzing population structure in this global pest.

Keywords

CYP303A1Helicoverpa armigera conferta
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 6 , December 2018 , pp. 817 - 830
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Copyright © Cambridge University Press 2018 

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