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Phlebotomus caucasicus and Phlebotomus mongolensis (Diptera: Psychodidae): indistinguishable by the mitochondrial cytochrome b gene in Iran

Published online by Cambridge University Press:  26 November 2009

P. Parvizi ,
H. Taherkhani  and
P.D. Ready
P. Parvizi*
Affiliation:
Molecular Systematics Laboratory, Pasteur Institute of Iran, Tehran, Iran Molecular Biology Laboratory, Department of Entomology, Natural History Museum, London, UK
H. Taherkhani
Affiliation:
Medical Faculty, University of Golastan Medical Sciences, Golastan, Iran
P.D. Ready
Affiliation:
Molecular Biology Laboratory, Department of Entomology, Natural History Museum, London, UK
*
*Author for correspondence Fax: +98 21 66469132 E-mail: [email protected]
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Abstract

Diagnostic molecular markers for the females of Phlebotomus (Paraphlebotomus) caucasicus and P. mongolensis were sought by characterizing from individual Iranian specimens a gene fragment, namely mitochondrial cytochrome b, that had previously proven useful for the taxonomy of phlebotomine sandflies. Males of both species were used as reference material because their external genitalia provide the only diagnostic morphological characters. A phylogenetic analysis of the new sequences, and those previously reported for P. grimmi, found no support for recognizing more than one species (P. caucasicus s.l.) in Iran. Most of the genetic variation was geographical. An absence of lineage sorting was demonstrated, and it is proposed that any search for species-specific molecular markers for these three taxonomic species should be continued by applied biologists only if there is better evidence for associating any one of them with phenotypes important for understanding the transmission of Leishmania species in foci of zoonotic cutaneous leishmaniasis.

Keywords

Phlebotomus caucasicusPhlebotomus mongolensisPhlebotomus grimmimitochondrial cytochrome bmolecular markerszoonotic cutaneous leishmaniasisIran
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 4 , August 2010 , pp. 415 - 420
Copyright
Copyright © Cambridge University Press 2009

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