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New microsatellites revealed strong gene flow among populations of a new outbreak pest, Athetis lepigone (Möschler)

Published online by Cambridge University Press:  27 November 2017

W.-C. Zhu ,
J.-T. Sun ,
J. Dai ,
J.-R. Huang ,
L. Chen  and
X.-Y. Hong
W.-C. Zhu
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
J.-T. Sun
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
J. Dai
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
J.-R. Huang
Affiliation:
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China
L. Chen
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
X.-Y. Hong*
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
*
*Author for correspondence Phone: +86-25-84395339 Fax: +86-25-84395339 E-mail: [email protected]
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Abstract

Athetis lepigone (Möschler) (Lepidoptera: Noctuidae) is a new outbreak pest in China. Consequently, it is unclear whether the emergence and spread of the outbreak of this pest are triggered by rapid in situ population size increases in each outbreak area, or by immigrants from a potential source area in China. In order to explore the outbreak process of this pest through a population genetics approach, we developed ten novel polymorphic expressed sequence tags (EST)-derived microsatellites. These new microsatellites had moderately high levels of polymorphism in the tested population. The number of alleles per locus ranged from 3 to 19, with an average of 8.6, and the expected heterozygosity ranged from 0.269 to 0.783. A preliminary population genetic analysis using these new microsatellites revealed a lack of population genetic structure in natural populations of A. lepigone. The estimates of recent migration rate revealed strong gene flow among populations. In conclusion, our study developed the first set of EST-microsatellite markers and shed a new light on the population genetic structure of this pest in China.

Keywords

SSRgenetic diversitygenetic structure
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 5 , October 2018 , pp. 636 - 644
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Copyright
Copyright © Cambridge University Press 2017 

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Footnotes

†

These authors contributed equally to this work.

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