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In vitro maintenance of Glossina palpalis palpalis (Robineau-Desvoidy) (Diptera: Glossinidae)

Published online by Cambridge University Press:  10 July 2009

B. Bauer
Affiliation:
International Atomic Energy Agency, Vienna, Austria
H. Aigner
Affiliation:
International Atomic Energy Agency, Vienna, Austria

Abstract

A silicone membrane was developed to meet the feeding requirements of Glossina palpalis palpalis (R.-D.). Starting with a total of 1050 females, a self-sustaining colony fed on defibrinated pig blood was established. The colony performance in terms of mean daily mortality, mean pupal weight and fecundity was evaluated. With the exception of June 1976, the mean daily mortality ranged between 0·40% and 1·20%. During June 1976, bacterial infections caused a sudden increase in mortality. Escherichia coli and Staphylococcus spp. were predominant in the gut of dead and diseased flies. Pure cultures of either organism caused the same symptoms in teneral flies within one week after uptake of an infected blood-meal. The weight of the offspring significantly decreased with increasing female age. Starting with a mean pupal weight of 30 mg there was a decrease of 4–6 mg over a period of 90 days. The innate capacity for increase in numbers (r-) was calculated based on data from 340 females. The value of rm = 0·0095 would result in a 32-fold increase of the colony in one year.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1978

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References

Bauer, B. & Wetzel, H. W. (1975). Effect of bacteria on tsetse flies fed through membranes, pp. 495499in Sterility principle for insect control. Proceedings of a Symposium jointly organised by the IAEA and FAO, Innsbruck, 1974.—622 pp. Vienna, International Atomic Energy Agency (Proceedings series STI/PUB/377).Google Scholar
Bauer, B. & Wetzel, H. (1976). A new membrane for feeding Glossina morsitans Westw. (Diptera, Glossinidae).—Bull. ent. Res. 65, 563565.CrossRefGoogle Scholar
Cavalli-Sforza, L. (1974). Biometrie. Grundzüge biologisch-medizinischer Statistik.—212 pp. Stuttgart, Gustav Fischer.Google Scholar
Dame, D. A. & Schmidt, C. H. (1970). The sterile-male technique against tsetse flies, Glossina spp.Bull. ent. Soc. Am. 16, 2430.Google Scholar
Evens, F. & Van Der Vloedt, A. (1970). The rearing of Glossina palpalis and Glossina quanzensis. pp. 97102 in de Azevedo, J. F. (Ed.). Tsetse fly breeding under laboratory conditions and its practical application. 1st international symposium 22 and 23 04 1969.—524 pp. Lisbon, Junta de Investigações do Ultramar.Google Scholar
Jordan, A. M. (1974). Recent developments in the ecology and methods of control of tsetse flies (Glossina spp.) (Dipt., Glossinidae)—a review.—Bull. ent. Res. 63, 361399.CrossRefGoogle Scholar
Jordan, A. M. & Curtis, C. F. (1972). Productivity of Giossina morsitans morsirans Westwood maintained in the laboratory, with particular reference to the sterile-insect release method.—Bull. Wld Hlth Org. 46, 3338.Google Scholar
Jordan, A. M. & Curtis, C. F. (1968). Productivity of Glossina austeni Newst. maintained on lop-eared rabbits.—Bull. ent. Res. 58, 399410.CrossRefGoogle Scholar
Krebs, C. J. (1972). Ecology. The experimental analysis of distribution and abundance.— 694 pp. London, Harper & Row.Google Scholar
Langley, P. A. (1972a). The role of physical and chemical stimuli in the development of in vitro feeding techniques for tsetse flies Glossina spp. (Dipt., Glossinidae).—Bull. ent. Res. 62, 215228.CrossRefGoogle Scholar
Langley, P. A. (1972b). Further experiments on rearing tsetse flies in the absence of a living host.—Trans. R. Soc. trop. Med. Hyg. 66, 310.CrossRefGoogle ScholarPubMed
Langley, P. A. & Maly, H. (1969). Membrane feeding technique for tsetse flies (Glossina spp.).—Nature, Lond. 221, 855856.CrossRefGoogle ScholarPubMed
Langley, P. A. & Pimley, R. W. (1973). Influence of diet composition on feeding and water excretion by the tsetse fly Glossina morsitans.—J. Insect Physiol. 19, 10971109.CrossRefGoogle ScholarPubMed
Lotka, A. J. (1925). Elements of physical biology. XXX.—460 pp. Baltimore, Md., Williams and Wilkins.Google Scholar
Mews, A. R. (1972). Supplementing the diet of membrane-fed G. morsitans by feeding on rabbits' ears once per week.—Trans. R. Soc. trop. Med. Hyg. 66, 306307.Google ScholarPubMed
Mews, A. R., Baumgartner, H., Luger, D. & Offori, E. D. (1976). Colonisation of Glossina morsitans morsitans Westw. (Diptera, Glossinidae) in the laboratory using in vitro feeding techniques.—Bull. ent. Res. 65, 631642.CrossRefGoogle Scholar
Mews, A. R., Langley, P. A., Pimley, R. W. & Flood, M. E. T. (1977). Large-scale rearing of tsetse flies (Glossina spp.) in the absence of a living host.—Bull. ent. Res. 67, 119128.CrossRefGoogle Scholar
Mews, A.R., Offori, E., Baumgartner, H. & Luger, D. (1972). Techniques used at the IAEA laboratory for rearing the tsetse fly Glossina morsitans Westwood, pp. 243254 in Proc. int. scient. Counc. Trypan. Res., 13th Meeting, Lagos, 1971.Google Scholar
Mews, A.R. &Ruhm, F. (1971). Maintenance of Glossina morsitans Westwood fed through an artificial membrane on defibrinated blood, pp. 283293 in Sterility principle for insect control or eradication. Proceedings of a symposium jointly organised by the IAEA and FAO, Athens, 1970.—542 pp. Vienna, International Atomic Energy Agency (Proceedings series STI/PUB/265).Google Scholar
Pagot, J., Itard, J. & Chomat, M. (1972). Utilisation d'une membrane synthétique pour la nourriture artificielle des glossines (Diptera–Muscidae).—C. r. hebd. Séanc. Acad. Sci., Paris 275D, 911912.Google Scholar
Sachs, L. (1972).Statistische Auswertungsmethoden.—3rd. edn. 545 pp. Berlin, Springer.Google Scholar
Van Der Vloedt, A. (1974). L'élevage au laboratoire de Glossina palpalis palpalis et de Glossina fuscipes quanzensis, pp. 6162 in Control programs for trypanosomes and their vectors. Proceedings of the V. Colloquium, Paris, 121503 1974.—387 pp. Maisons-Alfort, Institut d'Élevage et de Médecine Vétérinaire des Pays Tropicaux.Google Scholar
Weiser, J. & Briggs, J. D. (1971). Identification of pathogens, pp. 1366 in Burges, H. D. & Hussey, N. W. (Eds.). Microbial control of insects and mites.—861 pp. London, Academic Press.Google Scholar