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Host feeding patterns of Culicoides species (Diptera: Ceratopogonidae) within the Picos de Europa National Park in northern Spain

Published online by Cambridge University Press:  31 May 2012

J.H. Calvo ,
B. Berzal ,
C. Calvete ,
M.A. Miranda ,
R. Estrada  and
J. Lucientes
J.H. Calvo*
Affiliation:
Unidad Tecnología en Producción Animal. CITA, Zaragoza, Spain ARAID researcher
B. Berzal
Affiliation:
Unidad Tecnología en Producción Animal. CITA, Zaragoza, Spain
C. Calvete
Affiliation:
Unidad Sanidad Animal, CITA, Zaragoza, Spain
M.A. Miranda
Affiliation:
Laboratorio de Zoología, Universidad de las Islas Baleares, Palma de Mallorca, Spain
R. Estrada
Affiliation:
Departamento Patología Animal, Universidad de Zaragoza, Zaragoza, Spain
J. Lucientes
Affiliation:
Departamento Patología Animal, Universidad de Zaragoza, Zaragoza, Spain
*
*Author for correspondence Fax:976716335 E-mail: [email protected]
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Abstract

Blood meal identification can provide information about the natural host-feeding patterns or preferences of Culicoides species. Such information could indirectly provide data indicating which reservoirs are significant in associated vector-borne diseases.

We positively identified the host species through DNA sequencing of the cytochrome b gene in 144 of the 170 (84.7%) blood meal specimens tested. In the remaining samples, identification of the blood-meal source was unsuccessful, possibly due to the post-ingestion time prior to sampling or the availability of the species-specific cytochrome b gene sequences in the database. The majority of identified blood meals were derived from mammalian blood (95.8%), and only six contained chicken blood. We identified five species as mammalian hosts for Culicoides spp.: sheep (87.7%), human (6.5%), cattle (3.7%) and Savi's Pine Vole (Micrototus savii) (2.1%). The results suggested that large mammals, specifically ruminants, were most frequently fed upon by biting midges (Culicoides spp.), but evidence of opportunistic feeding behaviour was also found. Host feeding behaviour of Culicoides species may also be influenced by the relative abundance of a particular host species in the area being studied. In this sense, Savi's Pine Vole, a wild species, was found to be a locally relevant host and a putative reservoir for viruses transmitted by species of biting midges belonging to the Culicoides genus.

Finally, feeding on multiple potential host species was observed. One midge acquired blood meals from human and chicken hosts, while four other midges fed on two different sheep.

Keywords

CulicoidesPCRcytochrome b geneblood-meal
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 6 , December 2012 , pp. 692 - 697
Copyright
Copyright © Cambridge University Press 2012

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References

Arunachalam, N., Samuel, P.P., Hiriyan, J., Rajendran, R. & Dash, A.P. (2005) Short report: Observations on the multiple feeding behavior of Culex tritaeniorhynchus (Diptera: culicidae), the vector of Japanese encephalitis in Kerala in southern India. American Journal of Tropical Medicine and Higiene 72(2), 198–200.Google Scholar
Bartsch, S., Bauer, B., Wiemann, A., Clausen, P.H. & Steuber, S. (2009) Feeding patterns of biting midges of the Culicoides obsoletus and Culicoides pulicaris groups on selected farms in Brandenburg, Germany. Parasitology Research 105(2), 373–380.Google Scholar
Briegel, H. & Rezzonico, L. (1985) Concentration of host blood protein during feeding by anopheline mosquitoes (Diptera: Culicidae). Journal of Medical Entomology 22, 612–618.Google Scholar
Calvete, C., Calvo, J.H., Calavia, R., Miranda, M.A., Borrás, D., Estrada, R., Lucientes, J., Mañuz, B. & Romero, L. (2008) Culicoides species and transmission of bluetongue virus in Spain. Veterinary Record 162, 255.Google Scholar
De Liberato, C., Scavia, G., Lorenzetti, R., Scaramozzino, P., Amaddeo, D., Cardeti, G., Scicluna, M., Ferrari, G. & Autorino, G.L. (2005) Identification of Culicoides obsoletus (Diptera: Ceratopogonidae) as a vector of bluetongue virus in central Italy. The Veterinary Record 156, 301–304.Google Scholar
De Sebastián, J. (1997). Los aprovechamientos ganaderos en los Picos de Europa. pp. 63–76in Palacios, B., & Llaneza, L. (Eds) Primer Simposio sobre el Lobo en los Picos de Europa. Oviedo, Spain, Sociedad Española para la Conservación y Estudio de los Mamíferos (SECEM) – Grupo Lobo.Google Scholar
Du Toit, R.M. (1944) The transmission of blue-tongue and horsesickness by Culicoides. Onderstepoort Journal of Veterinary Science and Animal Industry 19, 7–16.Google Scholar
Gerry, A.C., Sarto i Monteys, V., Moreno Vidal, J.O., Francino, O. & Mullens, B.A. (2009) Biting rates of Culicoides midges (Diptera: Ceratopogonidae) on sheep in northeastern Spain in relation to midge capture using UV light and carbon dioxide-baited traps. Journal of Medical Entomology 46(3), 615–624.Google Scholar
Gyllensten, U., Wharton, D., Josefsson, A. & Wilson, A.C. (1991) Paternal inheritance of mitochondrial DNA in mice. Nature 352(6332), 255–257.Google Scholar
Haouas, N., Pesson, B., Boudabous, R., Dedet, J.P., Babba, H. & Ravel, C. (2007) Development of a molecular tool for the identification of Leishmania reservoir hosts by blood meal analysis in the insect vectors. American Journal of Tropical Medicine and Hygiene 77, 1054–1059.Google Scholar
Holmes, I.H., Boccardo, G., Estes, M.K. & Furuichi, M.K. (1995) Family Reoviridae. In Murphy, F.A., Fauquet, C.M., Bishop, D.H.L., Ghabrial, S.A., Jarvis, A.W., Martelli, G.P., Mayo, M.A. & Summers, M.D. (Eds) Virus Taxonomy: Classification and Nomeclature of Viruses. Sixth report of the International Committee on Taxonomy of Viruses. New York, USA, Springer, Archives of Virology (Supplement) 10, 208–239.Google Scholar
Kaneda, H., Hayashi, J., Takahama, S., Taya, C., Lindahl, K.F. & Yonekawa, H. (1995) Elimination of paternal mitochondrial DNA in intraspecific crosses during early mouse embryogenesis. Proceedings of the National Academy of Sciences of the United States of America 92(10), 4542–4546.Google Scholar
Kondo, R., Satta, Y., Matsuura, E.T., Ishiwa, H., Takahata, N. & Chigusa, S.I. (1990) Incomplete maternal transmission of mitochondrial DNA in Drosophila. Genetics 126(3), 657–663.Google Scholar
Lee, J.H., Hassan, H., Hill, G., Cupp, E.W., Higazi, T.B., Mitchell, C.J., GodseyJr, M.S. Jr, M.S. & Unnasch, T.R. (2002) Identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis. American Journal of Tropical Medicine and Hygiene 66(5), 599–604.Google Scholar
Maleki-Ravasan, N., Oshaghi, M.A., Javadian, E., Rassi, Y., Sadraei, J. & Mohtarami, F. (2009) Blood meal identification in field-captured sand flies: Comparison of PCR-RFLP and ELISA assays. Iranian Journal of Arthropod-Borne Disease 3(1), 8–18.Google Scholar
Mehlhorn, H., Walldorf, V., Klimpel, S., Jahn, B., Jaeger, F., Eschweiler, J., Hoffmann, B. & Beer, M. (2007) First occurrence of Culicoides obsoletus-transmitted bluetongue virus epidemic in central Europe. Parasitology Research 101, 219–228.CrossRefGoogle ScholarPubMed
Mehlhorn, H., Walldorf, V., Klimpel, S., Schaub, G., Kiel, E., Focke, R., Liebisch, G., Liebisch, A., Werner, D., Bauer, C., Clausen, H., Bauer, B., Geier, M., Hörbrand, T., Bätza, H.J., Conraths, F.J., Hoffmann, B. & Beer, M. (2009) Bluetongue disease in Germany (2007–2008): monitoring of entomological aspects. Parasitology Research 105(2), 313–319.Google Scholar
Meiswinkel, R., van Rijn, P., Leijs, P. & Goffredo, M. (2007) Potential new Culicoides vector of bluetongue virus in northern Europe. Veterinary Record 161, 564–565.Google Scholar
Meiswinkel, R., Baldet, T., de Deken, R., Takken, W., Delecolle, J.C. & Mellor, P.S. (2008) The 2006 outbreak of bluetongue in northern Europe – the entomological perspective. Preventive Veterinary Medicine 87(1–2), 55–63.Google Scholar
Mellor, P.S. (1996) Culicoides: vectors, climate change and disease risk. Veterinary Bulletin 66, 301–306.Google Scholar
Mellor, P.S. & Pitzolis, G. (1979) Observations on breeding sites and light trap collections of Culicoides during an outbreak of bluetongue in Cyprus. Bulletin of Entomological Research 69, 229–234.Google Scholar
Mellor, P.S., Boned, J., Hamblin, C. & Graham, S. (1990) Isolations of African horse sickness virus from vector insects made during the 1988 epizootic in Spain. Epidemiology and Infection 105, 447–454.Google Scholar
Molaei, G., Oliver, J., Andreadis, T.G., Armstrong, P.M. & Howard, J.J. (2006) Molecular identification of blood-meal sources in Culiseta melanura and Culiseta morsitans from an endemic focus of eastern equine encephalitis virus in New York. American Journal of Tropical Medicine and Hygiene 75(6), 1140–1147.CrossRefGoogle ScholarPubMed
Mukabana, W.R., Takken, W., Seda, P., Killeen, G.F., Hawley, W.A. & Knols, B.G. (2002) Extent of digestion affects the success of amplifying human DNA from blood meals of Anopheles gambiae (Diptera: Culicidae). Bulletin of Entomological Research 92(3), 233–239.Google Scholar
Mullens, B.A., Gerry, A.C., Monteys, V.S., Pinna, M. & González, A. (2010) Field studies on Culicoides (Diptera: Ceratopogonidae) activity and response to deltamethrin applications to sheep in northeastern Spain. Journal of Medical Entomology 47(1), 106–110.Google Scholar
Nielsen, S.A., Nielsen, B.O. & Chirico, J. (2010) Monitoring of biting midges (Diptera: Ceratopogonidae: Culicoides Latreille) on farms in Sweden during the emergence of the 2008 epidemic of bluetongue. Parasitology Research 106(5), 1197–1203.Google Scholar
Oshaghi, M.A., Chavshin, A.R., Vatandoost, H., Yaaghoobi, F., Mohtarami, F. & Noorjah, N. (2006) Effects of post-ingestion and physical conditions on PCR amplification of host blood meal DNA in mosquitoes. Experimental Parasitology 112(4), 232–236.Google Scholar
Oshaghi, M.A., Maleki Ravasan, N., Hide, M., Javadian, E., Rassi, Y., Sadraei, J., Mohebali, M., Sedaghat, M.M., Hajjaran, H., Zarei, Z. & Mohtarami, F. (2009) Vector incrimination of sand flies in the most important visceral leishmaniasis focus in Iran. American Journal of Tropical Medicine and Hygiene 81, 572–577.Google Scholar
Rawlings, P. (1996) A key, based on wing patterns of biting midges (genus Culicoides Latreille – Diptera: Ceratopogonidae) in the Iberian Peninsula, for use in epidemiological studies. Graellsia 52, 57–71.Google Scholar
Rawson, P.D., Secor, C.L. & Hilbish, T.J. (1996) The effects of natural hybridization on the regulation of doubly uniparental mtDNA inheritance in blue mussels (Mytilus spp.). Genetics 144(1), 241–248.Google Scholar
Takamatsu, H., Mellor, P.S., Mertens, P.P.C., Kirkham, P.A., Burroughs, J.N. & Parkhouse, R.M.E. (2003) A possible overwintering mechanism for bluetongue virus in the absence of the insect vector. Journal of general Virology 84, 227–235.Google Scholar
Torr, S.J. & Hargrove, J.W. (1998) Factors affecting the landing and feeding responses of the tsetse fly Glossina pallidipes to a stationary ox. Medical and Veterinary Entomology 12, 196–207.Google Scholar
Venter, G.J., Paweska, J.T., Lunt, H., Mellor, P.S. & Carpenter, S. (2005) An alternative method of blood-feeding Culicoides imicola and other haematophagous Culicoides species for vector competence studies. Veterinary Parasitology 131(3–4), 331–335.Google Scholar
Zouros, E., Oberhauser Ball, A., Saavedra, C. & Freeman, K.R. (1994) An unusual type of mitochondrial DNA inheritance in the blue mussel Mytilus. Proceedings of the National Academy of Sciences of the United States of America 91(16), 7463–7467.Google Scholar