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Expression and characterization of a lipase-related protein in the malpighian tubules of the Chinese oak silkworm, Antheraea pernyi

Published online by Cambridge University Press:  14 June 2016

L. Wang ,
J. Li ,
X. Zhao ,
C. Qian ,
G. Wei ,
B. Zhu  and
C. Liu
L. Wang
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
J. Li
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
X. Zhao
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
C. Qian
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
G. Wei
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
B. Zhu
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
C. Liu*
Affiliation:
College of Life Science, Anhui Agricultural University, Hefei 230036, China
*
*Author for correspondence Phone: +86 551 6578-6360 Fax: +86 551 6578-6201 E-mail: cyschx@163.com
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Abstract

Lipases are ubiquitous enzymes in nature, which play a crucial role in fat metabolism by catalyzing the hydrolysis of triacylglycerol to free fatty acids and glycerol. However, reports concerning insect lipase are rare. In this study, we studied the expression and activity of a lipase-related protein from Antheraea pernyi (ApLRP). Recombinant ApLRP was expressed in Escherichia coli cells and used to raise rabbit anti-ApLRP polyclonal antibodies. ApLRP mRNA and protein expression were abundant in the midgut and malpighian tubules, respectively. After challenge with four different microorganisms (E. coli, Beauveria bassiana, Micrococcus luteus and nuclear polyhedrosis virus), the expression levels of ApLRP mRNA in midgut were inducted significantly compared with the control. The different pathogens induced different ApLRP gene expression patterns. The optimum temperature and pH for the enzyme's activity were 35°C and 7.0, respectively. ApLRP activity was stimulated in the presence of Mg2+, Na+, Ca2+ and b-mercaptoethanol; while Zn2+, Cu2+ and Fe3+ inhibited its activity. Detergents such as SDS, glycerol and Tween-20 increased the lipase activity by 20–30%. Our results indicated that ApLRP might play an important role in the innate immunity of insects.

Keywords

Antheraea pernyilipaseimmunityactivity
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 5 , October 2016 , pp. 615 - 623
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Copyright
Copyright © Cambridge University Press 2016 

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