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Effects of insecticides chlorpyrifos, emamectin benzoate and fipronil on Spodoptera litura might be mediated by OBPs and CSPs

Published online by Cambridge University Press:  04 December 2017

X. Lin ,
Y. Jiang ,
L. Zhang  and
Y. Cai
X. Lin*
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Y. Jiang
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
L. Zhang
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Y. Cai
Affiliation:
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
*
*Author for correspondence Phone: +86-139-5802-8822 Fax: +86-571-86914449 E-mail: [email protected]
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Abstract

Spodoptera litura is a widespread polyphagous insect pest that can develop resistance and cross-resistance to insecticides, making it difficult to control. Insecticide exposure has previously been linked with induction of specific olfactory-related proteins, including some chemosensory proteins (CSPs) and odorant-binding proteins (OPBs), which may disrupt detection of environmental factors and reduce fitness. However, functional evidence supporting insecticide and OBPs/CSPs mediation remains unknown. Here we fed male S. litura moths with sucrose water containing one of three insecticides, chlorpyrifos, emamectin benzoate or fipronil, and used real-time quantitative polymerase chain reaction and RNAi to investigate OBPs and CSPs expression and their correlations with survival. Chlorpyrifos and emamectin benzoate increased expression of 78% of OBPs, plus 63 and 56% of CSP genes, respectively, indicating a major impact on these gene families. RNAi knockdown of SlituCSP18, followed by feeding with chlorpyrifos or fipronil, decreased survival rates of male moths significantly compared with controls. Survival rate also decreased significantly with the downregulation of SlituOBP9 followed by feeding with chlorpyrifos. Thus, although these three insecticides had different effects on OBP and CSP gene expression, we hypothesize that SlituOBPs and SlituCSPs might mediate their effects by increasing their expression levels to improve survival. Moreover, the differential response of S. litura male moths to the three insecticides indicated the potential specificity of chlorpyrifos affect SlituCSP18 and SlituOBP9 expression.

Keywords

Spodoptera liturachlorpyrifosemamectin benzoatefipronil
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 5 , October 2018 , pp. 658 - 666
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Copyright
Copyright © Cambridge University Press 2017 

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