Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-25T17:54:20.877Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of DNA probes for the identification of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex

Published online by Cambridge University Press:  10 July 2009

Maya B. Gunasekera ,
B.G.D.N.K. de Silva ,
W. Abeyewickreme ,
S.K. Subbarao ,
H.G. Nandadasa  and
E.H. Karunanayake
Maya B. Gunasekera*
Affiliation:
Department of Chemistry, Faculty of Science, University of Colombo, Sri Lanka
B.G.D.N.K. de Silva
Affiliation:
Faculty of Applied Science, University of Sri Jayewardenepura, Sri Lanka
W. Abeyewickreme
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Colombo, Sri Lanka
S.K. Subbarao
Affiliation:
Malaria Research Centre, Delhi, India
H.G. Nandadasa
Affiliation:
Faculty of Applied Science, University of Sri Jayewardenepura, Sri Lanka
E.H. Karunanayake
Affiliation:
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka
*
Dr Maya B. Gunasekera, Department of Chemistry, Faculty of Science, University of Colombo, Colombo 3, Sri Lanka.
Get access Rights & Permissions [Opens in a new window]

Abstract

Three highly repetitive DNA sequences Rp36, Rp217 and Rp234, have been isolated from Anopheles culicifacies Giles sensu lato. The cloned DNA sequences were found at a higher copy number in species B and C, than in species A of the A. culicifacies complex. These sequences may therefore be used as DNA probes to distinguish species A from the other two species, using a 200-fold dilution of a single mosquito DNA extract in a dot-blot hybridization assay. Rp36 and Rp217 have been completely sequenced. Internal repeats were absent in Rp36. Two related core sequences of 13 and 16 bp were found tandemly repeated in Rp217. These probes enable the rapid detection of species A of A. culicifacies in field investigations.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 85 , Issue 3 , September 1995 , pp. 345 - 353
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abhayawardana, T.A. (1993) Identification of sibling species of Anopheles culicifacies and An. subpictus complex and their distribution in Sri Lanka. World Health Organization Regional Publication, South -East Asian Series 4, 56–58.Google Scholar
Ainsley, R.W. (1976) Laboratory colonization of the malaria vector Anopheles culicifacies. Mosquito News 36, 256–258.Google Scholar
Akoh, J.I., Beidas, M.F. & White, G.B. (1984) Cytotaxonomic evidence for the malaria vector species A of the Anopheles culicifacies complex being endemic in Arabia. Transactions of the Royal Society of Tropical Medicine & Hygiene 78, 698.Google Scholar
Baimai, V. (1988) Population cytogenetics of the malaria vector Anopheles leucosphyrus group. Southeast Asian Journal of Tropical Medicine & Public Health 19, 667–679.Google ScholarPubMed
Besansky, N.J. & Collins, F.H. (1992) The mosquito genome organization, evolution and manipulations. Parasitology Today 8, 186–192.CrossRefGoogle Scholar
Booth, D.R., Mahon, R.J. & Sriprakash, K.S. (1991) DNA probes to identify the members of the Anopheles farauti complex. Medical & Veterinary Entomology 5, 447–451.Google ScholarPubMed
Collins, F.H., Mendez, M.E., Rasmussen, M.O., Mehaffy, P.C., Besansky, N.J. & Finnerty, V. (1987) A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. American Journal of Tropical Medicine & Hygiene 37, 37–41.CrossRefGoogle ScholarPubMed
Coluzzi, M. (1992) Malaria vector analysis and control. Parasitology Today 8, 113–118.CrossRefGoogle ScholarPubMed
Cooper, L., Cooper, R.D. & Burkot, T.R. (1991) The Anopheles punctulatus complex: DNA probes for identifying the Australian species using isotopic, chromogenic and chemiluminiscence detection system. Experimental Parasitology 73, 27–35.CrossRefGoogle Scholar
de Silva, B.G.D.N.K., Gunasekera, M.B., Abeyewickreme, W. & Karunanayake, E.H. (1993) Field analysis of Anopheles culicifacies Giles complex for the presence of sibling species A. Proceedings of the Sri Lanka Association for the Advancement of Science 49, 24.Google Scholar
Feinberg, A.P. & Vogelstein, B. (1983) A technique for radiolabelling DNA restriction endonuclease to high specific activity. Analytical Biochemistry 132, 6–13.CrossRefGoogle ScholarPubMed
Gale, K.P. & Crampton, J.M. (1987a) DNA probes for species identification of mosquitoes in the Anopheles gambiae complex. Medical & Veterinary Entomology 1, 127–136.CrossRefGoogle ScholarPubMed
Gale, K.P. & Crampton, J.M. (1987b) A DNA probe to distinguish the species Anopheles quadriannulatus from other species of the Anopheles gambiae complex. Transactions of the Royal Society of Tropical Medicine & Hygiene 81, 842–846.CrossRefGoogle ScholarPubMed
Green, C.A. & Miles, S.J. (1980) Chromosomal evidence for sibling species of the malaria vector Anopheles (Cellia) culicifacies. Journal of Tropical Medicine & Hygiene 83, 75–78.Google ScholarPubMed
Hill, S.M., Urwin, R., Knapp, T.F. & Crampton, J.M. (1991a) Synthetic DNA probes for the identification of sibling species in the Anopheles gambiae complex. Medical & Veterinary Entomology 1, 127–136.Google Scholar
Hill, S.M., Urwin, R. & Crampton, J.M. (1991b) A comparison of non-radioactive labelling and detection systems with synthetic oligonucleotide probes for the species identification of mosquitoes in the Anopheles gambiae complex. American Journal of Tropical Medicine & Hygiene 44, 609–622.Google ScholarPubMed
Mahmood, F., Sakai, R.K. & Akhtar, K. (1984) Vector incrimination studies and observations on species A and B of the taxon Anopheles culicifacies in Pakistan. Transactions of the Royal Society of Tropical Medicine & Hygiene 78, 607–616.CrossRefGoogle Scholar
Maniatis, T., Fritsch, E.F. & Sambrook, J. (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory.Google Scholar
Milligan, P.J.M., Phillips, A., Molyneux, D.H., Subbarao, S.K. & White, G.B. (1986) Differentiation of Anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components. Bulletin of Entomological Research 76, 529–537.Google Scholar
Panyim, S., Yasothornsrikul, S., Tungpradubkul, S., Baimai, V., Rosenberg, R., Andre, R.G. & Green, C.A. (1988) Identification of isomorphic malaria vectors using a DNA probe. American Journal of Tropical Medicine & Hygiene 38, 47–49.CrossRefGoogle ScholarPubMed
Paskewitz, S.M. & Collins, S.H. (1990) Use of polymerase chain reaction to identify mosquito species of the Anopheles gambiae complex. Medical & Veterinary Entomology 4, 367–373.CrossRefGoogle ScholarPubMed
Sanger, F., Nicklen, S. & Coulson, A.R. (1977) DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–5467.CrossRefGoogle ScholarPubMed
Scaife, J.G. & Goman, M. (1985) Construction of DNA library with phages. pp. 101–141in Panyim, S., Wilairat, P. & Yuthavong, Y. (Eds) Application of genetic engineering to research on tropical diseases pathogens with special reference to plasmodia: a laboratory manual of selected techniques. World Health Organization Publication. Bangkok.Google Scholar
Southern, E.M. (1979) Standard procedure for the transfer of DNA from agarose gels. Methods in Enzymology 68, 158–159.Google Scholar
Subbarao, S.K., Vasantha, K., Adak, T. & Sharma, V.P. (1983) Anopheles culicifacies complex. Evidence for a new sibling species C. Annals of the Entomological Society of America 76, 985–988.Google Scholar
Subbarao, S.K. (1988) The Anopheles culicifacies complex and control of malaria. Parasitology Today 4, 72–75.CrossRefGoogle ScholarPubMed
Subbarao, S.K., Vasantha, K. & Sharma, V.P. (1988) Cytotaxonomy of malaria vectors in India. pp. 25–27 in Service, M.W. (Ed.) Biosystematics of haematophagus insects. Oxford, Oxford University Press.Google Scholar
Subbarao, S.K., Nanda, N., Chandrahas, R.K. & Sharma, V.P. (1993) Anopheles culicifacies complex: cytogenetic characterization of Rameshwaram island populations. Journal of the American Mosquito Control Association 9, 27–31.Google ScholarPubMed
Suguna, S.G., Tewari, S.C., Mani, T.R., Hiriyan, J. & Reuben, R. (1983) Anopheles culicifacies species complex in Thenpennaiyar riverine tract, Tamil Nadu. Indian Journal of Medical Research 77, 455–459.Google ScholarPubMed
Suguna, S.G., Tewari, S.C., Mani, T.R., Hiriyan, J. & Reuben, R. (1989) A cytogenetic description of a new species of the Anopheles culicifacies complex. Genetica 78, 225–230.Google Scholar
Vasantha, K., Subbarao, S.K., Adak, T. & Sharma, V.P. (1983) Anopheles culicifacies: mitotic karyotype of species C. Indian Journal of Malariology 20, 161–162.Google Scholar
Vasantha, K., Subbarao, S.K. & Sharma, V.P. (1991) Anopheles culicifacies complex: population cytogenetic evidence for species D (Diptera: Culicidae). Annals of the Entomological Society of America 84, 531–536.CrossRefGoogle Scholar
Wickramasinghe, M.B. & Samarasinghe, L. (1991) Forest related malaria in Sri Lanka and prospects of its control. pp. 171–186 in Sharma, V.P. & Knodrashin, A.V. (Eds) Proceedings of an informal consultative meeting on forest malaria in south-east Asia. New Delhi, World Health Organization/Medical Research Council.Google Scholar
Yanisch-Perron, C., Vieira, J. & Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUCI9 vectors. Gene 33, 103–119.CrossRefGoogle ScholarPubMed