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Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugens Stål)

Published online by Cambridge University Press:  06 September 2011

S. Jing ,
B. Liu ,
L. Peng ,
X. Peng ,
L. Zhu ,
Q. Fu  and
G. He
S. Jing
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
B. Liu
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
L. Peng
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
X. Peng
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
L. Zhu
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
Q. Fu
Affiliation:
China National Rice Research Institute, Hangzhou 310006, People's Republic of China
G. He*
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, People's Republic of China
*
*Author for correspondence Fax: +86 27 68752327 E-mail: [email protected]
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Abstract

To assess genetic diversity in populations of the brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae), we have developed and applied microsatellite, or simple sequence repeat (SSR), markers from expressed sequence tags (ESTs). We found that the brown planthopper clusters of ESTs were rich in SSRs with unique frequencies and distributions of SSR motifs. Three hundred and fifty-one EST-SSR markers were developed and yielded clear bands from samples of four brown planthopper populations. High cross-species transferability of these markers was detected in the closely related planthopper N. muiri. The newly developed EST-SSR markers provided sufficient resolution to distinguish within and among biotypes. Analyses based on SSR data revealed host resistance-based genetic differentiation among different brown planthopper populations; the genetic diversity of populations feeding on susceptible rice varieties was lower than that of populations feeding on resistant rice varieties. This is the first large-scale development of brown planthopper SSR markers, which will be useful for future molecular genetics and genomics studies of this serious agricultural pest.

Keywords

brown planthoppersimple sequence repeatexpress sequence taggenetic diversitybiotype
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 102 , Issue 1 , February 2012 , pp. 113 - 122
Copyright
Copyright © Cambridge University Press 2011

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