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Characterization of three heat shock protein 70 genes from Liriomyza trifolii and expression during thermal stress and insect development

Published online by Cambridge University Press:  10 May 2018

Y.-W. Chang ,
X.-X. Zhang ,
J.-Y. Chen ,
M.-X. Lu ,
W.-R. Gong  and
Y.-Z. Du
Y.-W. Chang
Affiliation:
School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China
X.-X. Zhang
Affiliation:
School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China
J.-Y. Chen
Affiliation:
School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China Laboratory for Prevention and Control of Alien Pests, Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou 215000, China
M.-X. Lu
Affiliation:
School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China
W.-R. Gong
Affiliation:
Plant Protection and Quarantine Station of Jiangsu Province, Nanjing 21003, China
Y.-Z. Du*
Affiliation:
School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education, Yangzhou University, Yangzhou 25009, China
*
*Author for correspondence Phone: 086-514-87971854 Fax: 086-514-87347537 E-mail: [email protected]
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Abstract

Heat shock proteins (HSPs) participate in diverse physiological processes in insects, and HSP70 is one of the most highly conserved proteins in the HSP family. In this study, full-length cDNAs of three HSP70 genes (Lthsc70, Lthsp701, and Lthsp702) were cloned and characterized from Liriomyza trifolii, an important invasive pest of vegetable crops and horticultural crops worldwide. These three HSP70s exhibited signature sequences and motifs that are typical of the HSP70 family. The expression patterns of the three Lthsp70s during temperature stress and in different insect development stages were studied by real-time quantitative PCR. Lthsp701 was strongly induced by high- and low-temperature stress, but Lthsc70 and Lthsp702 were not very sensitive to temperature changes. All three Lthsp70s were expressed during insect development stages, but the expression patterns were quite different. The expression of Lthsc70 and Lthsp702 showed significant differences in expression during leafminer development; Lthsc70 was most highly expressed in female adults, whereas Lthsp702 was abundantly expressed in larvae and prepupae. Lthsp701 expression was not significantly different among leafminer stages. These results suggest that functional differentiation within the LtHSP70 subfamily has occurred in response to thermal stress and insect development.

Keywords

Liriomyza trifoliiheat shock protein 70thermal stressdevelopmental stageexpression profile
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 109 , Issue 2 , April 2019 , pp. 150 - 159
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Copyright © Cambridge University Press 2018 

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