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Changes occurred in the testes and DNA pattern of males wax moth (Galleria mellonella) first generation as a result of irradiation of their parents

Published online by Cambridge University Press:  15 December 2016

Salwa Abdo Rizk ,
Ragaa Sayed Abdalla  and
Rehab Mahmoud Sayed
Salwa Abdo Rizk
Affiliation:
Natural Product Department, National Center of Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Ragaa Sayed Abdalla
Affiliation:
Natural Product Department, National Center of Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Rehab Mahmoud Sayed*
Affiliation:
Natural Product Department, National Center of Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
*
*Author for correspondence Phone: 01156662204 Fax: (+202) 22749298 E-mail: [email protected]
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Abstract

Nowadays, the sterile insect technique is broadly used as a pest control measure. Therefore, the present study was conducted to investigate the alteration occurred in testes and DNA pattern as an effect of inherited sterility. Full grown pupae of the wax moth, Galleria mellonella were irradiated with 80 and 160 Gy of γ irradiation. The size of the testes was decreased by increasing of γ irradiation dose. Also, the size of the testes was decreased in F1 males comparing with the size of the testes of both the parents and the untreated control. The effects of γ rays on the DNA patterns of adult male parents and F1 males showed alterations among the controls, the treated parents and F1 individuals. Exposure to radiation caused very frequently the appearance of some extra bands and the deficiency of others in the arbitrary random amplified polymorphic DNA-polymerase chain reaction amplification patterns of the irradiated insects.

Keywords

wax mothmale reproductive systemRAPD-PCRγ radiation
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 4 , August 2017 , pp. 493 - 498
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Copyright
Copyright © Cambridge University Press 2016 

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References

Abd-El Aziz, M.F. & Haiba, I.M. (2008) Effect of γ radiation on the genomic DNA of potato tuber moth, Phthorimaea operculella Zeller (Lepidoptera – Gelechiidae). Isotope and Radiation Research 40(3), 779–791.Google Scholar
Abdel Baki, S. & Al Khalaf, A.A. (2011) The effect of γ irradiation on the ovaries and testes of Plodiainterpunctella (Phycitidae: Lepidoptera). Journal of Agricultural Biotechnology and Sustainable Development 3(6), 105–107.Google Scholar
Abulyazid, I., Ibrahim, S.M., El-Shikh, M.A. & Abdlhamid, W.A. (2007) Effect of pupal substerilizing doses (200 and 250 Gy) upon the genetic material of the cotton leaf moth Spodoptera littoralis (Lepidoptera: Noctuidae). Journal of Egyptian Academic Society for Environmental Development 8(1), 19–27.Google Scholar
Alexandrov, D.A., Lapidus, I.L., Alexandrov, M.V., Korablinova, S.V. & Levkovich, N.V. (1998) Rapid screening of spontaneous and radiation-induced structural changes at the vestigial gene of Drosophila melanogaster by polymerase chain reaction. Journal of Rapid Communication 66(4), 33–38.Google Scholar
Atienzar, F.A. & Jha, A.N. (2006) The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: a critical review. Mutation Research 613, 76–102.Google Scholar
Atienzar, F.A., Cheung, V.V., Jha, A.N. & Depledge, M.H. (2001) Fitness parameters and DNA effects are sensitive indicators of copper induced toxicity in Daphnia manga . Toxicological Sciences 59, 241–250.CrossRefGoogle Scholar
Black, W.C., Nancy, M.D., Gary, J.P., James, R.N. & Jennifer, M.P. (1992) Use of the random amplified polymorphic DNA polymerase chain reaction (RAPDPCR) to detect DNA polymorphisms in aphids (Homoptera: Aphididae). Bulletin of Entomological Research 82(2), 151–159.Google Scholar
Carpenter, J.E., Bloem, S. & Marec, F. (2005) Inherited sterility in insects. pp. 115–146 in Dyck, V.A., Hendrichs, J. & Robinson, A.S. (Eds) Sterile Insect Technique, Dordrecht, The Netherlands, Springer.Google Scholar
Colter, D. (1995) Those pesky wax moths. Part 2: Controls. American Bee Journal 135(2), 121–122.Google Scholar
Ebadi, R. (1975) Wax of bee. Journal of Iranian Entomology 10(4), 61–64.Google Scholar
El-said, T.S. (2013) Inherited sterility in the cotton leaf worm Spodoptera littoralis, (Boisd.), and changes in the DNA pattern as a result of using γ radiation. Ph.D Thesis, Fac. Sci. Ain Shams University. PP 216–219. Available online at http://www.iaea.org/inis/collection/NCLCollectionStore/_Public/46/066/46066344.pdf Google Scholar
Ercan, F.S. (2015) Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean Flour Moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Archieves of Biological Science, Belgrade 67(1), 235–239.Google Scholar
Garner, K.J. & Slavicek, J.M. (1996) Identification and characterization of a RAPD-PCR marker for distinguishing Asian and North American gypsy moths. Insect Molecular Biology 5(2), 81–91.CrossRefGoogle ScholarPubMed
Humason, G.L. (1972) Animal Tissue Techniques. San Francisco, Freeman and Co. Ltd.Google Scholar
Jafari, R., Goldasteh, S. & Afrogheh, S. (2010) Control of the Wax Moth Galleria Mellonella L. (Lepidoptera: Pyralidae) by the Male Sterile Technique (MST). Archieves of Biological Science 62(2), 309–313.Google Scholar
Kumar, P., Kumar, M., Thakur, S., Rajneesh, , Mattu, V.K. & Seth, A. (2015) Phylogenetic analysis of Noctuoid moths (Lepidoptera: Noctuoidea) by RAPD-PCR technique. Asian Journal of Advanced Basic Sciences 4(1), 61–67.Google Scholar
LaChance, L.E. (1985) Genetic Methods for the Control of Lepidopteran Species: Status and Potential. ARS-28. Washington, DC, USA, USDA/ARS.CrossRefGoogle Scholar
Lea, D.E. (1956) Actions of Radiations on Living Cells. 2nd edn. Cambridge, the Cambridge University Press.Google Scholar
Nei, M. & Li, W.H. (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 76(10), 5269–5273.Google Scholar
North, D.T. (1975) Inherited sterility in Lepidoptera. Annual Review of Entomology 20, 167–182.Google Scholar
Sallam, H.A., AbdelL-Gawad, A.A., Assar, M.M., Ibrahim, S.M. & EL-Naggar, S.M. (1996) Histological changes in the testes of γ irradiated cotton leafworm Spodoptera littoralis (Boisd). Proceedings of the 6th conference of nuclear sciences and applications. 15–20 Mar 1996. Available online at http://www.iaea.org/inis/collection/NCLCollectionStore/_Public/28/030/28030279.pdf?r=1 Google Scholar
Sallam, H.A., El-Dossouki, S.A., El-Naggar, S.E.M. & Shibel, M.M. (2000) Effect of γ irradiation and dose accumulation on the histology of Spodoptera littoralis (Boisd.) Male Testes B.A. Seventh Conference of Nuclear Sciences & Applications 6–10 February 2000. Cairo, Egypt. Available online at http://www.iaea.org/inis/collection/NCLCollectionStore/_Public/32/016/32016630.pdf Google Scholar
Sayed, R.M. (2008) Effect of γ radiation and entomopathogenic nematodes on greater wax moth, Galleria mellonella (Linnaeus) [Lep., Pyralidae]. MSc Thesis, Fac. Sc. Ain Shams University Available online at http://www.iaea.org/inis/collection/NCLCollectionStore/_Public/40/026/40026052.pdf Google Scholar
Seth, R.K., Rao, D.K. & Kaur, J.J. (2000) Developmental pattern of testes in F1 progeny of γ irradiated Spodoptera litura (Fabr.). Journal of Nuclear Agriculture and Biology 29(3–4), 129–141.Google Scholar
Singh, R., Bajpai, N. & Tewari, R.R. (2011) Genetic characterization of Bactrocera (Dacus) Flies (Diptera: Tephritidae) based on RAPD-PCR. International Journal of Pharma and Bio Sciences 2(2), B498–B503.Google Scholar
Tothová, A. & Marec, F. (2001) Chromosomal principle of radiation-induced F 1 sterility in Ephestia kuehniella (Lepidoptera: Pyralidae). Genome 44, 172–184.CrossRefGoogle Scholar
von Sonntag, C. (1987) New aspects in the free-radical chemistry of pyrimidine nucleobases. Free Radical Research Communications 2, 217–224.Google Scholar
Wiesner, A. (1993) Die Induktion der Immunabwehr eines Insekts (Galleria mellonella, Lepidoptera). Durch Synthetische Materialien und Arteigene Haemolymphfaktoren, PhD Thesis, in Berlin, 107 pp, Berlin.Google Scholar
Williams, J.K.W., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1990) DNA Polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18(22), 6531–6535.Google Scholar