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Screening of anaerobic gut fungi using a pressure transducer

Published online by Cambridge University Press:  27 February 2018

W.-Y. Zhu ,
M. K. Theodorou ,
B. B. Nielsen  and
A. P. J. Trinci
W.-Y. Zhu
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
M. K. Theodorou
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
B. B. Nielsen
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB School of Biological Sciences, 1800 Stopford Building, University of Manchester, Manchester M13 9PL
A. P. J. Trinci
Affiliation:
School of Biological Sciences, 1800 Stopford Building, University of Manchester, Manchester M13 9PL
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Extract

The growth of gut anaerobic fungi is usually determined in batch culture by gravimetric measurements of dry-matter (DM) loss, or by quantification of fermentation end-products (Lowe et al., 1987). These techniques involve destructive sampling of the cultures and require many replicate cultures in time-course experiments. Moreover, techniques using DM loss are only able to measure the end-point of degradation of substrate and cannot follow the growth over the entire fermentation period. Using a pressure transducer, Theodorou et al. (1994) developed a simple and sensitive gas production procedure to determine the fermentation kinetics of ruminant foods. This procedure can also be used to follow the growth of anaerobic fungi on soluble and particulate substrate in batch cultures Theodorou et al. (1995).

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Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 275 - 277
Copyright
Copyright © British Society of Animal Science 1998

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References

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