Hostname: page-component-6bf8c574d5-k2jvg Total loading time: 0 Render date: 2025-03-09T23:20:22.231Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of nitrogen form on growth of rumen micro-organism in vitro

Published online by Cambridge University Press:  27 February 2018

M. D. Carro  and
E. L. Miller
M. D. Carro
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
E. L. Miller
Affiliation:
Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 OJQ
Get access

Extract

Rumen microbes utilize mainly ammonia as a nitrogen (N) source for their growth but some species also use a variety of amino acids (AA) or peptides. Several studies have shown both an enhanced fibre digestion and efficiency of rumen micro-organisms when N sources such as AA, peptides and protein were provided in addition to ammonia (Griswold et al.,1996). However, no difference either in digestion or in growth of rumen microbes was found in other in vivo(Fujimaki et al.,1989) and in vitro (Kernick, 1991) studies. The objective of this experiment was to investigate the effects of protein, peptides, AA and ammonia on microbial growth and fermentation of an all fibre basal diet in a semicontinuous culture system (RUSITEC; Czerkawski and Breckenridge, 1977).

Type
Posters
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 309 - 311
Copyright
Copyright © British Society of Animal Science 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agricultural and Food Research Council. 1993. Energy and protein requirements of ruminants. An advisory manual prepared by the AFRC Technical Committee on Responses to Nutrients. CAB International, Wallingford.Google Scholar
Cruz Soto, R., Muhammed, Samirah A., Newbold, C. J., Stewart, C. S. and Wallace, R. J. 1994. Influence of peptides, amino acids and urea on microbial activity in the rumen of sheep receiving grass hay and on the growth of rumen bacteria in vitro. Animal Feed Science and Technology 49: 151–161.Google Scholar
Czerkawski, J. W. and Breckenridge, G. 1977. Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition 38: 371–384.Google Scholar
Fujimaki, T., Kobayashi, M., Wakita, M. and Hoshino, S. 1989. Influence of amino acid supplement on cellulolysis and microbial yield in sheep rumen. Journal of Animal Physiology and Animal Nutrition 62: 119–124.Google Scholar
Griswold, K. E., Hoover, W. H., Miller, T. K. and Thayne, W. V. 1996. Effect of form of nitrogen on growth of ruminal microbes in continuous culture. Journal of Animal Science 74: 483–491.CrossRefGoogle ScholarPubMed
Kernick, B. L. 1991. The effect of form of nitrogen on the efficiency of protein synthesis by rumen bacteria in continuous culture. Ph.D. dissertation. University of Natal, Pietermaritzburg, South Africa.Google Scholar
McDougall, E.I. 1948. Studies on ruminant saliva. I. The composition and output of sheep's saliva. Biochemistry Journal 43: 99–109.Google Scholar
Mehrez, A. Z. and Ørskov, E. R. 1977. A study of the artificial fibre bag technique for determining the digestibility of feeds in the rumen. Journal of Agricultural Science, Cambridge 88: 645–650.Google Scholar
Merry, R. J., McAllan, A. B. and Smith, R. H. 1990. In vitro continuous culture studies on the effect of nitrogen source on rumen microbial growth and fibre digestion. Animal Feed Science and Technology 31: 55–64.Google Scholar
Statisical Analysis Systems Institute. 1993. SAS companion for the Microsoft Windows environment, version 6. SAS Institute Inc. Cary, NC. Google Scholar