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Use of in vitro digestion by protease from Streptomyces griseus, ficin or pancreatin for the estimation of ruminal effective degradability of dried lucerne forage

Published online by Cambridge University Press:  27 February 2018

A. Antoniewicz  and
I. Kosmala
A. Antoniewicz
Affiliation:
National Research Institute on Animal Production, Sarego 2, 31-047 Kraków, Poland
I. Kosmala
Affiliation:
National Research Institute on Animal Production, Sarego 2, 31-047 Kraków, Poland
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Extract

Lucerne forage can provide significant amounts of both degraded and undegraded protein to ruminants. Changes in chemical composition taking place during advancement of maturity also affect ruminal degradability of protein. Therefore, forages harvested in a wide range of vegetation stages represent a good set of samples suitable to test and validate in vitro methods. The aim of the study was to elaborate the best conditions of using protease from Streptomyces griseus (PSG) to estimate ruminal degradability of dried lucerne forage and compare the results with those obtained when the same material was digested with ficin (FIC) or pancreatin (PNC).

Type
Use of enzymes in in vitro methods
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 118 - 119
Copyright
Copyright © British Society of Animal Science 1998

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References

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