Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-23T17:14:18.329Z Has data issue: false hasContentIssue false
  • English
  • Français

Vitamin-A-dependent zinc-binding protein and intestinal absorption of Zn in chicks

Published online by Cambridge University Press:  09 March 2007

Nadezhda I. Berzin  and
Victoria K. Bauman
Nadezhda I. Berzin
Affiliation:
Institute of Biology, Salaspils, Latvian SSR, 229021, USSR
Victoria K. Bauman
Affiliation:
Institute of Biology, Salaspils, Latvian SSR, 229021, USSR
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. It was demonstrated that the development of experimental avitaminosis A in chicks led to secondary zinc deficiency. The balance of Zn in the chick became negative, while the Zn content of various tissues decreased. Thus in vitamin-A-deficient chicks the serum Zn content was 1258 (SD 26.3) μg/l which was considerably lower than 1652 (SD 97.8) μg/l in controls.

2. Zn absorption was considerably reduced throughout the entire small intestine of vitamin-A-deficient chicks and most markedly in the ileal region. Within 72 h after retinyl acetate administration Zn absorption was fully restored in this region of the intestine.

3. The 65Zn-binding capacity of soluble proteins, present in the supernatant fraction of ileal-mucosa homogenates of vitamin-A-deficient chicks, was found to increase 2.6 times by 72 h after the administration of a single dose of retinyl acetate.

4. A vitamin-A-dependent Zn-binding protein (ZnBP), absent in vitamin-A-deficient chicks, was isolated from the ileal mucosa after their repletion with vitamin A. Competitive-binding studies (calcium, cadmium, copper) showed the protein to be highly specific for Zn ions.

5. The molecular weight of ZnBP wa5 83 kDa. The association constant of the protein-Zn complex was 0.8 x 106/mol. The protein was acidic with approximately 20% of its amino acid residues belonging to dicarboxylic acids. ZnBP was found to be a glycoprotein, and it contained hexose as a carbohydrate component.

6. It is suggested that ZnBP is involved in the binding of Zn in the ileal mucosa of chicks.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 57 , Issue 2 , March 1987 , pp. 255 - 268
Copyright
Copyright © The Nutrition Society 1987

References

REFERENCES

Bauman, V. K. & Berzin, N. I. (1976). Prikladnaya Biokhimia i Microbiologia N 12, 151–156.Google Scholar
Berzin, N. I. (1981). In Fundamental Problems of Gastroenterology, pp. 37–38 [Bogach, P. T., editor]. Kiev: State University of Kiev.Google Scholar
Berzin, N. I. & Bauman, V. K. (1982). In Biochemistry of Nutrient Absorption in Animals, pp. 68–76. [Valdman, A. R., editor]. Riga: Zinatne.Google Scholar
Bruckental, J., Ascarelli, I. & Bondi, A. (1974). British Journal of Nutrition 31, 1–11.CrossRefGoogle Scholar
Colowick, S. P. & Womack, F. C. (1969). Journal of Biological Chemistry 244, 774–777.CrossRefGoogle Scholar
Dahmer, E. J. (1971). Dissertation Abstracts International 31, 45.Google Scholar
Dimitrovsky, A. A. & Kryukov, V. S. (1975). Quantitative Determination of Vitamin A by Use of Thin Layer Chromatography (Methods), pp. 11–12. Moscow: Nauka.Google Scholar
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956). Analytical Chemistry 28, 350–356.CrossRefGoogle Scholar
Eichhorn, F., Zelmanowsky, S., Lew, E., Rutenberg, A. & Fanias, B. (1961). Journal of Clinical Pathology 14, 450–452.CrossRefGoogle Scholar
Evans, G. W. (1980). Nutrition Reviews 38, 137–141.CrossRefGoogle Scholar
Evans, G. W. & Johnson, P. E. (1977). American Journal of Clinical Nutrition 30, 611.Google Scholar
Evans, G. W. & Johnson, E. C. (1981). Journal of Nutrition 111, 68–75.CrossRefGoogle Scholar
Forbes, R. M., Parker, M. M. & Erdman, J. L. (1984). Journal of Nutrition 114, 1421–1425.CrossRefGoogle Scholar
Gornal, A. G., Bardavill, C. J. & David, M. M. (1949). Journal of Biological Chemistry 177, 751–766.CrossRefGoogle Scholar
Hansen, M. A, Fernandes, G. & Good, R. A. (1982). Annual Review of Nutrition 2, 151–177.CrossRefGoogle Scholar
Holbrook, J. B. & Leaver, A. G. (1976). Analytical Biochemistry 75, 634–636.CrossRefGoogle Scholar
House, W. A., Welch, R. M. & Van Campen, D. R. (1982). Journal of Nutrition 112, 941–953.CrossRefGoogle Scholar
Jackson, M. J., Jones, D. A. & Edwards, R. H. T. (1981). British Journal of Nutrition 46, 15–27.CrossRefGoogle Scholar
King, J. & Laemli, U. K. (1971). Journal of Molecular Biology 62, 465–477.CrossRefGoogle Scholar
Kowarski, S., Blair-Stanek, C. S. & Schachter, D. (1974). American Journal of Physiology 226, 401–407.CrossRefGoogle Scholar
Lönnerdal, B. O., Keen, C. L., Sloan, M. V. & Hurley, L. S. (1980). Journal of Nutrition 110, 2414–2419.CrossRefGoogle Scholar
Menard, M. P. & Cousins, R. J. (1983). Journal of Nutrition 113, 1653–1656.CrossRefGoogle Scholar
Perkin-Elmer Corporation (1976). Analytical Methods for Atomic Absorption Spectrophotometry. Norwalk, Connecticut: The Perkin-Elmer Corporation.Google Scholar
Richards, M. P. & Cousins, R. J. (1977). Biochemical and Biophysical Research Communications 75, 286–294.CrossRefGoogle Scholar
Sahagian, B. M., Harding-Barlow, I. & Perry, H. M. (1967). Journal of Nutrition 93, 291–300.CrossRefGoogle Scholar
Schwarz, F. J. & Kirchgessner, M. (1975). Zeitschrqt für Tierphysiologie, Tierernährung und Futtermittelkunde 35, 257–266.CrossRefGoogle Scholar
Solomons, N. W. M. D. (1982). American Journal of Clinical Nutrition 35, 1048–1075.CrossRefGoogle Scholar
Spackman, D. M., Stein, W. H. & Moore, S. (1958). Analytical Chemistry 30, 1190–1206.CrossRefGoogle Scholar
Starcher, B. C., Glauber, J. G. & Madaras, J. G. (1980). Journal of Nutrition 110, 1391–1397.CrossRefGoogle Scholar
Ueng, T. H. & Bronner, F. (1979). Archives of Biochemistry and Biophysics 197, 205–217.CrossRefGoogle Scholar
Ugolev, A. M., Zhigure, D. R. & Nurks, J. J. (1970). Fiziologischesky Zhurnal SSSR 56, 1638–1641.Google Scholar
Valinietse, M. Y., Bauman, V. K., Berzin, N. I., Zhigure, D. R. & Jansone, L. A. (1975). In Absorption and Metabolism of Nutrients in Animal Organisms, pp. 159–167 [Valdman, A. R., editor]. Riga: Zinatne.Google Scholar
Van Campen, D. R. & Kowalski, T. J. (1971). Proceedings of the Society for Experimental Biology and Medicine 136, 294–297.CrossRefGoogle Scholar
Wasserman, R. M., Corradino, R. A. & Taylor, A. N. (1968). Journal of Biological Chemistry 243, 3978–3986.CrossRefGoogle Scholar
Zhigure, D. R. & Babarikin, D. A. (1975). In Absorption and Metabolism of Nutrients in Animal Organisms, pp. 28–37. [Valdman, A. R., editor]. Riga: Zinatne.Google Scholar