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Vegetable lipid sources in vitro biosyntheis of triacylglycerols and phospholipids in the intestine of sea bream (Sparus aurata)

Published online by Cambridge University Press:  08 March 2007

Maria José Caballero ,
Germ´an Gallardo ,
Lidia Robaina ,
Daniel Montero ,
Antonio Fernández  and
Marisol Izquierdo
Maria José Caballero*
Affiliation:
Department of Comparative Pathology, Trasmontaña, s/n, 35416 Arucas, Las Palmas de Gran Canaria, Canary Islands, Spain
Germ´an Gallardo
Affiliation:
Department of Biochemical, Cellular Biology and Physiology, Edificio Ciencias de la Salud, Campus Universitario de San Cristóbal, Las Palmas de Gran Canaria, Canary IslandsSpain
Lidia Robaina
Affiliation:
Grupo de Investigación en Acuicultura, ULPGC & ICCM, PO Box 56, 35200, Telde, Las Palmas de Gran Canaria, Canary Islands, Spain
Daniel Montero
Affiliation:
Grupo de Investigación en Acuicultura, ULPGC & ICCM, PO Box 56, 35200, Telde, Las Palmas de Gran Canaria, Canary Islands, Spain
Antonio Fernández
Affiliation:
Department of Comparative Pathology, Trasmontaña, s/n, 35416 Arucas, Las Palmas de Gran Canaria, Canary Islands, Spain
Marisol Izquierdo
Affiliation:
Grupo de Investigación en Acuicultura, ULPGC & ICCM, PO Box 56, 35200, Telde, Las Palmas de Gran Canaria, Canary Islands, Spain
*
*Corresponding authorfax +34 928451141, email [email protected]
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Abstract

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Despite the good growth performance of several fish species when dietary fish oil is partly replaced by vegetable oils, recent studies have reported several types of intestinal morphological alterations in cultured fish fed high contents of vegetable lipid sources. However, the physiological process implied in these morphological changes have not been clarified yet, since alterations in the physiological mechanisms involved in the different processes of lipid absorption could be responsible for such gut morphological features. The objective of the present study was to investigate the activities of reacylation pathways in fish, the glycerol-3-phosphate and the monoacylglycerol pathways, in order to clarify the intestinal triacylglycerol (TAG) and phospholipid biosynthesis to better understand the morphological alterations observed in the intestine of fish fed vegetable oils. Intestinal microsomes of sea bream fed different lipid sources (fish, soyabean and rapeseed oils) at three different inclusion levels were isolated and incubated with l-[14C(U)]glycerol-3-phosphate and [1-14C]palmitoyl CoA. The results showed that in this fish species the glycerol-3-phosphate pathway is mainly involved in phospholipid synthesis, whereas TAG synthesis is mainly mediated by the monoacylglycerol pathway. Feeding with rapeseed oil reduced the reacylation activity in both pathways, explaining the high accumulation of lipid droplets in the supranuclear portion of the intestinal epithelium, whereas soyabean oil enhanced phosphatidylcholine synthesis, being associated with the increase in VLDL found in previous studies.

Keywords

Intestinal microsomesVegetable oilsSea breamMonoacylglycerol pathwayGlycerol-3-phosphate pathwayCytidine diphosphate nucleotidesTriacylglycerolsPhospholipids
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 448 - 454
Copyright
Copyright © The Nutrition Society 2006

References

Bell, JGMcGhee, FCampbell, PJSargent, JR 2003 Rapeseed oil as an alternative to marine fish oil in diets of post-smolt Atlantic salmon (Salmo salar): changes in flesh fatty acid composition and effectiveness of subsequent fish oil ‘wash out’. Aquaculture 218 515–528.CrossRefGoogle Scholar
Caballero, MJIzquierdo, MSKjørsvik, EFernández, AJRosenlund, G 2004 Histological alterations in liver of sea bream (Sparus aurata) caused by feeding for a short or long-term with vegetable oils. Recovery of normal morphology after feeding fish oil as a solely lipid source. J Fish Dis 27 531–541.Google Scholar
Caballero, MJIzquierdo, MSKjørsvik, EMontero, DSocorro, JFernández, AJRosenlund, G 2003 Morphological aspects of intestinal cells from gilthead seabream (Sparus aurata) fed diets containing different lipid sources. Aquaculture 225 325–340.Google Scholar
Caballero, MJObach, ARosenlund, GMontero, DGisvold, MIzquierdo, MS 2002 Impact of different dietary lipid sourceson growth, lipid digestibility, tissue fatty acid composition and histology of rainbow trout. Oncorhynchus mykiss. Aquaculture 214 253–271.Google Scholar
Carman, GM 1997 Phosphatidate phosphatases and diacylglycerol pyrophosphate phosphatases in Sacchromyces cerevisiae and Escherichia coli. Biochim Biophys Acta 1348 45–55.CrossRefGoogle Scholar
Coleman, RALewin, TMMuoio, D 2000 Physiological and nutritional regulation of enzymes of triacylglycerol synthesis. Annu Rev Nutr 20 77–103.Google Scholar
Folch, JLees, MSloane-Stanley, GH 1957 A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226 497–509.CrossRefGoogle ScholarPubMed
Hatch, GMMcClarty, G 1996 Regulation of cardiolipin biosynthesis in H9C2 cardiac myoblasts by cytidine 50-triphosphate. J Biol Chem 271 25810–25816.CrossRefGoogle Scholar
Iijima, NZama, K & Kayama, M 1993 Effect of the oxidized lipids on the metabolic pathway of lipid biosynthesis in the intestine of carp (Cyprinus carpio). Nippon Suisan Gakkaishi 49 1465–1470.Google Scholar
Iijima, NTanaka, S & Ota, YPurification and characterization of bile-salt activated lipase from the hepatopancreas of red sea bream, Pargus major. Fish Physiol Biochem 1998 18 56–61.Google Scholar
Izquierdo, MSHenderson, RJ 1998 The determination of lipase and phospholipase activities in gut contents of turbot (Scophtalmus maximus) by fluorescent-based assays. Fish Physiol Biochem 19 153–162.Google Scholar
Izquierdo, MSMontero, DRobaina, LCaballero, MJRosenlund, GGinés, R 2005 Alterations in fillet fatty acid profile and flesh quality in gilthead sea bream (Sparus aurata) fed vegetable oils for a longterm period. Recovery of fatty acids profile by fish oil. Aquaculture 250 431–444.CrossRefGoogle Scholar
Izquierdo, MSObach, AArantzamendi, LRobaina, LMontero, DRosenlund, G 2003 Dietary lipid sources for seabream and seabass:growth performance, tissue composition and flesh quality. Aquacult Nutr 9 397–407.CrossRefGoogle Scholar
Izquierdo, MS, Socorro, JArantzamendi, L & Hernández-Cruz, CM 2000 Recent advances in lipid nutrition in fish larvae. Fish Physiol Biochem 22 97–107.Google Scholar
Johnston, JMPaltauf, FSchiller, CMSchultz, LD 1970 The utilization of the a-glycerophosphate and monoglyceride pathways for phosphatidylcholine biosynthesis in the intestine. Biochim Biophys Acta 218 124–133.CrossRefGoogle Scholar
Johnston, JMRaO, GALowe, PA 1967 The separation of the a-glycerophosphate and monoglyceride pathways in the intestinal biosynthesis of triglycerides. Biochim Biophys Acta 137 578–580.CrossRefGoogle Scholar
Lehner, RKuksis, A 1995 Triacylglycerol synthesis by purified triacylglycerol synthetase of rat intestinal mucosa: role of acyl-CoA acyltransferase. Biol Chem 270 13630–13636.CrossRefGoogle ScholarPubMed
Lowry, OHRosebrough, NJFarr, ALRandall, RJ 1951 Protein measurement with folin phenol reagent. J Biol Chem 193 265–275.CrossRefGoogle ScholarPubMed
Mansbach, CMTso, PKuksis, AIntestinal Lipid Metabolism. New York: Kluwer Academic. 2001CrossRefGoogle Scholar
Nutrient Requirements of Fish Washington: DC: National Academy Press. 1993Google Scholar
Olsen, REMyklebust, RKaino, TRingø, E 1999 Lipid digestibility and ultrastructural changes in the enterocytes of Arctic charr (Salvelinus alpinus L.) fed linseed oil and soybean lecithin. Fish Physiol Biochem 21 35–44.CrossRefGoogle Scholar
Olsen, REMyklebust, RKaino, TRingø, EMayhew, TM 2000 The influences of dietary linseed oil and saturated fatty acids on caecal enterocytes in Arctic charr (Salvelinus alpinus L.) a quantitative ultrastructural study. Fish Physiol Biochem 22 207–216.CrossRefGoogle Scholar
Regost, cArzel, JRobin, JRosenlund, GKaushik, SJ 2003 Total replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima): 1. Growth performance, flesh fatty acid profile, and lipid metabolism. Aquaculture 217 465482.CrossRefGoogle Scholar
Sire, MFLutton, CVernier, JM 1981 New views on intestinal absorption of lipids in teleostean fishes: an ultrastructural and biochemical study in the rainbow trou. J Lipid Res 22 81–94.CrossRefGoogle Scholar
Sokal, RRRolf, FJBiometry. The Principles and Practice of Statistics in Biological Research 3rd ed, New York, WH Freeman & Co. 1995Google Scholar
Stals, HKMannaerts, GPDeclercq, PE 1992 Factors influencing triacylglycerol synthesis in permeabilized rat hepatocytes. Biochem J 283 719–725.Google Scholar
Tijburg, LBMHuelen, MJHVan Golde, LMG 1989 Regulation of the biosynthesis of triacylglycerol, phosphatidylcholine and phosphatidylethanolamine in the liver. Biochim Biophys Acta 1004 1–19.CrossRefGoogle ScholarPubMed