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Utilization of tryptophan, nicotinamide and nicotinic acid as precursors for nicotinamide nucleotide synthesis in isolated rat liver cells

Published online by Cambridge University Press:  09 March 2007

David A. Bender  and
Ronald Olufunwa
David A. Bender
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WC1E 6BT
Ronald Olufunwa
Affiliation:
Department of Biochemistry, University College and Middlesex School of Medicine, University College London, Gower Street, London WC1E 6BT
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Abstract

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1. Incubation of isolated rat hepatocytes with nicotinamide or nicotinic acid showed that while both vitamers were taken up from the incubation medium, neither was utilized to any significant extent as a precursor of the nicotinamide nucleotide coenzymes, NAD and NADP, and neither was capable of preventing the loss of nucleotides that occurs on incubating the cells.

2. Incubation of hepatocytes with tryptophan showed that de novo synthesis from tryptophan permitted replacement of the nucleotides lost during incubation; at high concentrations of tryptophan there was an increase above the initial intracellular concentration of NAD(P). Incubation of hepatocytes with tryptophan also resulted in the formation and release from the cells of a considerable amount of niacin, as well as the two principal metabolities of NAD(P), N1-methyl nicotinamide and methyl pyridone carboxamide.

3. It is suggested that, in the liver, preformed niacin is not utilized for nucleotide synthesis, and indeed the function of the liver appears to be synthesis of niacin from tryptophan, and its release for use by extrahepatic tissues that lack the pathway for de novo synthesis of nicotinamide nucleotides from tryptophan.

Type
General Nutrition Papers
Information
British Journal of Nutrition , Volume 59 , Issue 2 , March 1988 , pp. 279 - 287
Copyright
Copyright © The Nutrition Society 1988

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