Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-15T17:16:43.015Z Has data issue: false hasContentIssue false
  • English
  • Français

Utilization of ileal digestible amino acids by growing pigs: tryptophan

Published online by Cambridge University Press:  17 March 2008

E. S. Batterham ,
L. M. Andersen  and
D. R. Baigent
E. S. Batterham
Affiliation:
NSW Agriculture, Wollongbar Agricultural Institute, Wollongbar, New South Wales 2477, Australia
L. M. Andersen
Affiliation:
NSW Agriculture, Wollongbar Agricultural Institute, Wollongbar, New South Wales 2477, Australia
D. R. Baigent
Affiliation:
NSW Agriculture, Wollongbar Agricultural Institute, Wollongbar, New South Wales 2477, Australia
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The ileal digestibility of tryptophan for growing pigs was determined for cottonseed, meat-and-bone and soya-bean meals. Tryptophan in the food and digesta was measured by two analytical procedures (NaOH hydrolysis and colorimetric estimation (method 1) and LiOH hydrolysis and HPLC determination (method 2)). The results were respectively: cottonseed meal 0.46, 0.81; meat-and-bone meal 0.55, 0.65; soya-bean meal 0.74, 0.90. In the first experiment the values for method 1 were shown to be inapplicable to pigs. In a second experiment three tryptophan-deficient diets (005 g ileal digestible tryptophan/MJ digestible energy (DE)) were formulated using values from method 2 for cottonseed meal, meat-and-bone meal plus L-tryptophan and soya-bean meal respectively as the only sources of tryptophan in the diets. This experiment was terminated after 28 d as overall growth performance of the pigs was very low. A third experiment was conducted in a similar manner to Expt 2 except that the diets were formulated to 0.065g ileal digestible tryptophan/MJ DE and growth responses and tryptophan retention were assessed over the 20–45 kg growth phase. Growth rates (g.d) of the pigs given the three diets were significantly different (P < 0.01): cottonseed meal 393, meat-and-bone meal plus L-tryptophan 531, soya-bean meal 437 (SED 39.0). Tryptophan retention (as a proportion of ileal digestible tryptophan intake) was significantly different (P < 0.05): cottonseed meal 0.51, meat-and-bone meal plus L-tryptophan 0.49, soya-bean meal 0.41. These results indicate (1) that the colorimetric technique for assessing tryptophan was inapplicable and (2) that ileal digestible values for tryptophan were not suitable for formulating diets containing heat-processed proteins, possibly due to absorption of some of the tryptophan in a form that was non-utilizable, and/or to underestimation of total tryptophan in the protein concentrates.

Keywords

TryptophanIleal digestibilityPigs
Type
Utilization of trytophan derived from ileal digestion
Information
British Journal of Nutrition , Volume 71 , Issue 3 , March 1994 , pp. 345 - 360
Copyright
Copyright © The Nutrition Society 1994

References

REFERENCES

Agricultural Research Council (1981). The Nutrient Requirements of Pigs. Slough: Commonwealth Agricultural Bureaux.Google Scholar
Batterham, E. S., Andersen, L. M. & Baigent, D. R. (1993). Utilization of ileal digestible amino acids by growing pigs: methionine. British Journal of Nutrition 70, 711–720.CrossRefGoogle ScholarPubMed
Batterham, E. S., Andersen, L. M., Baigent, D. R., Beech, S. A. & Elliott, R. (1990 a). Utilization ofileal digestible amino acids by pigs: lysine. British Journal of Nutrition 64, 679–690.CrossRefGoogle Scholar
Batterham, E. S., Andersen, L. M., Baigent, D. R. & White, E. (1990 b). Utilization of ileal digestible amino acids by growing pigs: effect of dietary lysine concentration on efficiency of lysine retention. British Journal of Nutrition 64, 81–94.CrossRefGoogle ScholarPubMed
Batterham, E. S. & Murison, R. D. (1981). Utilization of free lysine by growing pigs. British Journal of Nutrition 46, 87–92.CrossRefGoogle ScholarPubMed
Beech, S. A., Batterham, E. S. & Elliott, R. (1991). Utilization of ileal digestible amino acids by pigs: threonine. British Journal of Nutrition 65, 381–390.CrossRefGoogle ScholarPubMed
Black, J. L., Campbell, R. G., Williams, I. H., James, K. J. & Davies, G. T. (1986). Simulation of energy and amino acid utilization in the pig. Research and Development in Agriculture 3, 121–145.Google Scholar
Burlacu, G., Baia, G., Ionila, D., Moisa, D., Tascenco, V., Visan, I. & Stoica, I. (1973). Efficiency of the utilization of the energy of food in piglets after weaning. Journal of Agricultttruf Science, Cumbridge 81, 295–302.Google Scholar
Campbell, R. G., Taverner, M. R. & Rayner, C. J. (1988). The tissue and dietary protein and amino acid requirements of pigs from 8.0 to 20.0 kg live weight. Animal Production 46, 283–290.Google Scholar
Degussa AG (1986). Determination of Raw Materials and Feedstuffs After Alkaline Hydro1.vsis and HPLC Detection. Analysis, A5. Hanau: Degussa AG.Google Scholar
Fuller, M. F. & Wang, T. C. (1987). Amino acid requirements of the growing pig. In Manipulating Pig Production, pp. 97–111 [APSA Committee, , editors]. Werribee: Australasian Pig Science Association.Google Scholar
Hugli, T. E. & Moore, S. (1972). Determination of the tryptophan content of proteins by ion exchange chromatography of alkaline hydrolysates. Journal of Biological Chemistry 247, 2828–2834.CrossRefGoogle ScholarPubMed
Jordan, J. W. & Brown, W. O. (1970). The retention of energy and protein in the baby pig fed on cows' milk. In Energy Metabolism of Farm Animals, pp. 161–164 [Schurch, A and Wenk, C, editors]. Zurich: Juris Druck and Verlag.Google Scholar
Lenis, N. P., van Diepen, J.Th. M. & Goedhart, P. W. (1990). Amino acid requirements of pigs. 1. Requirements for methionine +cystine, threonine and tryptophan of fast-growing boars and gilts, fed ad libitum. Netherlands. Journal of Agricultural Science 81, 577–595.Google Scholar
Standing Committee on Agriculture (1987). Feeding Standards for Ausfralian Livestock. Pigs. East Melbourne: CSIRO.Google Scholar
Tanksley, T. D. & Knabe, D. A. (1981). Use of cottonseed meal in swine rations. Feedstuffs 53, 24–27.Google Scholar