Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-26T13:15:05.441Z Has data issue: false hasContentIssue false
  • English
  • Français

The urinary excretion of aromatic acids by starved sheep

Published online by Cambridge University Press:  09 March 2007

A. A. Martin
A. A. Martin
Affiliation:
Hannah Dairy Research Institute, Ayr
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Four wether sheep were maintained on a diet of hay for 2 weeks and then starved for a period of 4 days.

2. Immediately before and during starvation the urinary excretion in the following fractions was determined: hippuric acid, creatinine, total diethyl ether-soluble acids of hydrolysed and unhydrolysed urine, total aromatic acids in hydrolysed and unhydrolysed urine and the proportion of the former present as benzoic and phenylacetic acids.

3. A method for determining the benzoic acid content of light petroleum extracts of urine has been developed and is described.

4. Starvation had little effect on the urinary excretion of phenylacetic acid or creatinine, but during the first 2 days of starvation there were large decreases in the excretion of all the other urinary fractions studied.

5. Of the fractions examined, 43% of the diethyl ether-soluble acids of hydrolysed urine and 42% of those of unhydrolysed urine were of exogenous origin; 76% of the total urinary aromatic acids were of exogenous origin. Partition of the aromatic acids in the urine of two of the four sheep indicated that the reduction in aromatic acid excretion on starvation was completely accounted for by the decline in benzoic acid output. Almost all the hippuric acid (97%) was of exogenous origin.

6. These results have been compared with the urinary output of aromatic acids by nonruminants when fasted, and possible reasons for the relatively large amounts of phenylacetic acid found in the urine of starved sheep have been discussed.

Type
Research Article
Information
British Journal of Nutrition , Volume 23 , Issue 3 , August 1969 , pp. 715 - 725
Copyright
Copyright © The Nutrition Society 1969

References

Armstrong, M. D., Chao, F.-C., Parker, V. J. & Wall, P. E. (1955). Proc. Soc. exp. Biol. Med. 90, 675.CrossRefGoogle Scholar
Asakawa, T., Wada, H. & Yamano, T. (1968). Biochim. biophys. Acta 170, 375.Google Scholar
Bernard, K., Vuilleumier, J. P. & Brubacher, G. (1955). Helv. chim. Acta 38, 1438.CrossRefGoogle Scholar
Blaxter, K. L. (1962). Br. J. Nutr. 16, 615.CrossRefGoogle Scholar
Bray, H. G., Thorpe, W. V. & White, K. (1951). Biochem. J. 48, 88.CrossRefGoogle Scholar
Bruns, F. H. & Fiedler, L. (1958). Nature, Lond. 181, 1533.CrossRefGoogle Scholar
Bruns, F. H., Haberland, G. L. & Altman, K. I. (1959). Biochem. Z. 331, 446.Google Scholar
Gaffney, G. W., Schreier, K., DiFerrante, N. & Altman, K. I. (1954). J. biol. Chem. 206, 695.CrossRefGoogle Scholar
Kumler, W. D. & Strait, L. A. (1943). J. Am. chem. Soc. 65, 2349.Google Scholar
McEvoy-Bowe, E. (1966). Analyt, Biochem. 16, 153.Google Scholar
Martin, A. K. (1966). Br. J. Nutr. 20, 325.CrossRefGoogle Scholar
Martin, A. K. (1969). Br. J. Nutr. 23, 289.Google Scholar
Meister, A. (1965). Biochemistry of the Amino Acids, 2nd ed., p. 152. London: Academic Press Inc.Google Scholar
Min, B. H. & Schreiber, E. C. (1966). J. Chromat. 24, 463.CrossRefGoogle Scholar
Moldave, K. & Meister, A. (1957). Biochem. biophys. Acta 24, 654.CrossRefGoogle Scholar
Patton, S. & Kesler, E. M (1967). J. Dairy Sci. 50, 1505.Google Scholar
Schreier, K., Altman, K. I. & Hempelmann, L. H. (1954). Proc. Soc. exp. Biol. Med. 87, 61.Google Scholar
Scott, T. W., Ward, P. F. V. & Dawson, R. M. C. (1964). Biochem. J. 90, 12.Google Scholar
Snedecor, G. W. (1940). Statistical Methods, 3rd ed., p. 223. Ames, Iowa: Iowa College Press.Google Scholar
Stein, W. H., Paladini, A. C., Hirs, C. H. W. & Moore, S. (1954). J. Am. chem. Soc. 76, 2848.Google Scholar