Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-23T15:59:45.597Z Has data issue: false hasContentIssue false
  • English
  • Français

Tomatoes have natural anti-thrombotic effects

Published online by Cambridge University Press:  09 March 2007

J. Yamamoto ,
T. Taka ,
K. Yamada ,
Y. Ijiri ,
M. Murakami ,
Y. Hirata ,
A. Naemura ,
M. Hashimoto ,
T. Yamashita  and
K. Oiwa
...Show all authors
J. Yamamoto*
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
T. Taka
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
K. Yamada
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
Y. Ijiri
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
M. Murakami
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
Y. Hirata
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
A. Naemura
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
M. Hashimoto
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
T. Yamashita
Affiliation:
Laboratory of Physiology, Faculty of Nutrition and High Technology Research Centre, Kobe Gakuin University, Kobe, Japan
K. Oiwa
Affiliation:
Communications Research Laboratory, Kansai Advanced Research Centre, Kobe, Japan
J. Seki
Affiliation:
Biomedical Engineering, National Cardiovascular Centre, Osaka, Japan
H. Suganuma
Affiliation:
Research Institute, Kagome Co. Ltd., Tochigi, Japan
T. Inakuma
Affiliation:
Research Institute, Kagome Co. Ltd., Tochigi, Japan
T. Yoshida
Affiliation:
National Institute of Vegetable and Tea Science, National Agricultural Research Organization, Mie, Japan
*
*Corresponding Author: Dr J. Yamamoto, fax +81 78 974 5689, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The prevention of arterial thrombotic diseases has a high priority in developed countries. An inappropriate diet may be an important risk factor for thrombotic events. The daily intake of an anti-thrombotic diet may offer a convenient and effective way of prevention. The aim of the present study was to test tomato extracts for anti-thrombotic effects and to identify those varieties that have such an effect. A shear-induced platelet-function test (haemostatometry) was used to test anti-thrombotic potential in vitro. Extracts from those tomato varieties that showed a significant anti-thrombotic activity in vitro were further assessed in vivo, using a laser-induced thrombosis test in mice. One tomato variety (KG99-4) showed significant anti-thrombotic activity both in vitro and in vivo. KG99-4 inhibited not only platelet-rich thrombus formation but also had a thrombolytic effect. It is concluded that haemostatometry can detect and classify the anti-thrombotic potential of fruits and vegetables and offers a simple way of screening for such effects.

Keywords

TomatoesPlatelet aggregationThrombosisCardiovascular disease
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 6 , December 2003 , pp. 1031 - 1038
Copyright
Copyright © The Nutrition Society 2003

References

Arab, L & Steck, S (2000) Lycopene and cardiovascular diseases. Am J Clin Nutr 71, Suppl. 1691S–1695S.10.1093/ajcn/71.6.1691SCrossRefGoogle Scholar
Bazzano, LA, He, J, Ogden, LG, et al. (2002) Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first national health and nutrition examination survey epidemiologic follow-up study. Am J Clin Nutr 76, 93–99.CrossRefGoogle ScholarPubMed
Briggs, WH, Folts, JD, Osman, HE & Goldman, IL (2001) Administration of raw onion inhibits platelet-mediated thrombosis in dogs. J Nutr 131, 2619–2622.10.1093/jn/131.10.2619CrossRefGoogle ScholarPubMed
Brown, BG, Zhao, XQ, Chait, A, et al. . (2001) Simvastatin and niacin, antioxidant vitamins, or the combination for the prevention of coronary disease. N Engl J Med 345, 1583–1592.10.1056/NEJMoa011090CrossRefGoogle ScholarPubMed
Demrow, HS, Slane, PR & Folts, JD (1995) Administration of wine and grape juice inhibits in vivo platelet activity and thrombosis in stenosed canine coronary arteries. Circulation 91, 1182–1188.CrossRefGoogle ScholarPubMed
Dutta-Roy, AK, Crosbie, L & Gordon, MJ (2001) Effects of tomato extract on human platelet aggregation in vitro. Platelets 12, 218–227.Google ScholarPubMed
Fuster, V, Badimon, L, Badimon, JJ & Chesebro, JH, (1992) The pathogenesis of coronary artery disease and the acute coronary syndromes. N Engl J Med 326, 242–251.Google ScholarPubMed
Gillman, MW, Cupples, LA, Gagnon, D, et al. (1995) Protective effect of fruits and vegetables on development of stroke in men. JAMA 273, 1113–1117.10.1001/jama.1995.03520380049034CrossRefGoogle ScholarPubMed
Gorog, DA & Kovacs, IB (1995) Thrombotic status analyzer. Measurement of platelet-rich thrombus formation and lysis in native blood. Thromb Haemost 73, 514–520.Google ScholarPubMed
Gorog, P & Kovacs, IB (1990) Modelling coronary thrombosis from nonanticoagulated human blood in vitro. Hematol Pathol 4, 43–52.Google ScholarPubMed
Ijiri, Y, Miura, M, Hashimoto, M et al. . (2002) A new model to evaluate the diet-induced prothrombotic state, using He-Ne laser-induced thrombogenesis in the carotid artery of apolipoprotein receptor E-deficient and low-density lipoprotein receptor-deficient mice. Blood Coagul Fibrinolysis 13, 497–504.10.1097/00001721-200209000-00004CrossRefGoogle ScholarPubMed
Joshipura, KJ, Ascheio, A, Manson, JE, et al. (1999) Fruit and vegetable intake in relation to risk of ischemic stroke. JAMA 282, 1233–1239.CrossRefGoogle ScholarPubMed
Joshipura, KJ, Hu, FB, Manson, JE, et al. . (2001) The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med 134, 1106–1114.10.7326/0003-4819-134-12-200106190-00010CrossRefGoogle ScholarPubMed
Keevil, JG, Osman, HE, Reed, JD & Folts, JD (2000) Grape juice, but not orange juice or grapefruit juice, inhibits human platelet aggregation. J Nutr 130, 53–56.10.1093/jn/130.1.53CrossRefGoogle ScholarPubMed
Kovacs, IB, Hutton, RA & Kernoff, PB (1989) Hemostatic evaluation in bleeding disorders from native blood. Clinical experience with the hemostatometer. Am J Clin Pathol 91, 271–279.CrossRefGoogle ScholarPubMed
Kovacs, IB, Tigyi-Sebes, A, Trombitas, K & Gorog, P (1975) Evans blue: an ideal energy-absorbing material to produce intravascular microinjury by HE-NE gas laser. Microvasc Res 10, 107–124.10.1016/0026-2862(75)90025-4CrossRefGoogle ScholarPubMed
Liu, S, Manson, JE, Lee, I-M, et al. (2000) Fruit and vegetable intake and risk of cardiovascular disease: the women's health study. Am J Clin Nutr 72, 922–928.CrossRefGoogle ScholarPubMed
Mukamal, KJ, Conigrave, KM, Mittleman, MA, et al. (2003) Roles of drinking pattern and type of alcohol consumption in coronary heart disease in men. N Engl J Med 348, 9–18.10.1056/NEJMoa022095CrossRefGoogle ScholarPubMed
Nakajima, S, Noguchi, T, Taka, T, et al. (2000) A global platelet test of thrombosis and thrombolysis detects a prothrombotic state in some patients with non-insulin dependent diabetes and in some patients with stroke. Platelets 11, 459–466.CrossRefGoogle Scholar
Osman, HE, Maalej, N, Shanmuganayagam, D & Folts, JD (1998) Grape juice but not orange or grapefruit juice inhibits platelet activity in dogs and monkeys ( Macaca fasciularis ). J Nutr 128, 2307–2312.10.1093/jn/128.12.2307CrossRefGoogle Scholar
Ratnatunga, CP, Edmondson, SF, Rees, GM & Kovacs, IB (1992) High-dose aspirin inhibits shear-induced platelet reaction involving thrombin generation. Circulation 85, 1077–1082.10.1161/01.CIR.85.3.1077CrossRefGoogle ScholarPubMed
Renaud, S & de Lorgeril, M (1992) Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet 339 1523–1526.10.1016/0140-6736(92)91277-FCrossRefGoogle ScholarPubMed
Shanmuganayagam, D, Beahm, MR, Osman, HE, Krueger, GG, Reed, JD & Folts, JD (2002) Grape seed and grape skin extracts elicit a greater antiplatelet effect when used in combination than when used individually in dogs and humans. J Nutr 132, 3592–3598.10.1093/jn/132.12.3592CrossRefGoogle ScholarPubMed
Sixma, JJ (1994) Interaction of blood platelets with the vessel wall. In Haemostasis and Thrombosis 259–285 [Bloom, AL, Forbes, CD, Thomas, DP and Tuddenham, EGD, editors]. London: Churchill Livingstone.Google Scholar
Stein, JH, Keevil, JG, Wiebe, DA, Aeschlimann, S & Folts, JD (1999) Purple grape juice improves endothelial function and reduces the susceptibility of LDL cholesterol to oxidation in patients with coronary artery disease. Circulation 100, 1050–1055.CrossRefGoogle ScholarPubMed
Taka, T, Ono, H, Sasaki, Y, Seki, J & Yamamoto, J, (2002) Platelet reactivity in spontaneously diabetic rats is independent from blood glucose and insulin levels. Platelets 13, 313–316.10.1080/0953371027000-1CrossRefGoogle ScholarPubMed
Truelsen, T, Gronbaek, M, Schnohr, P & Boysen, G (1998) Intake of beer, wine, and spirits and risk of stroke: the Copenhagen city heart study. Stroke 29, 2467–2472.10.1161/01.STR.29.12.2467CrossRefGoogle ScholarPubMed
Ulbricht, TL & Southgate, DA (1991) Coronary heart disease: seven dietary factors. Lancet 338, 985–992.CrossRefGoogle ScholarPubMed
Yamamoto, J, Iizumi, H, Hirota, R, et al. . (1989) Effect of physical training on thrombotic tendency in rats: decrease in thrombotic tendency measured by the He-Ne laser-induced thrombus formation method. Haemostasis 19, 260–265.Google ScholarPubMed
Yamamoto, J, Ishii, I, Okita, N, et al. . (1997) The differential involvement of von Willebrand factor, fibrinogen and fibronectin in acute experimental thrombosis in rat cerebral and mesenteric microvessels. Jpn J Physiol 47, 431–441.10.2170/jjphysiol.47.431CrossRefGoogle ScholarPubMed
Yamamoto, J, Yamashita, T, Ikarugi, H, et al. (2003) Gorog thrombosis test: a global in vitro test of platelet function and thrombolysis. Blood Coagul Fibrinolysis 14, 31–39.10.1097/00001721-200301000-00007CrossRefGoogle Scholar