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Supplemental vitamin A enhances the recovery from iron deficiency in rats with chronic vitamin A deficiency

Published online by Cambridge University Press:  09 March 2007

AnnetJ.C Roodenburg ,
Clive E West ,
Robert Hovenierl  and
Anton C Beynen
AnnetJ.C Roodenburg
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, Wageningen, The Netherlands Department of Laboratory Animal Science, State University Utrecht, The Netherlands
Clive E West
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, Wageningen, The Netherlands Center for International Health, Rollins School of Public Health, Emory University, 1518 Clgton Road N.E., Atlanta GA 30322, USA
Robert Hovenierl
Affiliation:
Department of Human Nutrition, Wageningen Agricultural University, Wageningen, The Netherlands
Anton C Beynen
Affiliation:
Department of Laboratory Animal Science, State University Utrecht, The Netherlands
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Abstract

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Studies with anaemic children and pregnant women from areas where vitamin A deficiency is endemic have shown a beneficial effect on Fe status of supplemental vitamin A in addition to Fe supplementation. This suggests a relationship between vitamin Aand Fe status, which we attempted to mimic in rats with anaemia and chronic vitaminA deficiency. Male rats were fed on Fe-adequate diets (35 mg Fe/kg)containing different levels of vitamin A (1200,450,150,75 and 0 retinol equivalents (RE)/kg feed) until they were 5 weeks old. These diets wereidentical to the diets fed to their mothers. Then the young male rats were transferred to diets containing the same levels of vitamin A but no added Fe. After another 2 weeks the rats wererepleted with Fe (35 mg/kg feed) without or with vitamin A to a level of 1200 RE/kg feed. Increased vitamin A intake by the groups previously fed on diets with either 0 or 75 RE/kg produced a reduction in blood haemoglobin concentration, packed cell volume and erythrocyte count. In the group which had been fed on the diet without vitamin A, supplemental vitamin A raised mean cell volume, plasma Fe concentration and total Fe-binding capacity. Vitamin A supplementation during the period of Fe repletion produceda decrease in splenic and tibia Fe concentration, the effect being greater with increasing seventy of previous vitamin A deficiency. The paradoxical effect of supplemental vitamin A on haemoglobin, packed cell volume and erythrocyte count can be explained by a decrease in the degree of haemwoncentration. Thus, the positive effect of supplemental vitamin A seen in humans is also observed with rats under controlled experimental conditions. We speculate that supplemental vitamin A during Fe repletion contributes to optimum erythropoiesis and Fe mobilization when baseline vitamin A status is impaired

Keywords

Vitamin AIronrats
Type
Vitamin A metabolism in rats
Information
British Journal of Nutrition , Volume 75 , Issue 4 , April 1996 , pp. 623 - 636
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Amine, E. K., Corey, J., Hegsted, D. M. & Hayes, K.C.(1970). Comparative hematology during deficiencies of iron and vitamin A in the rat. Journal of Nutrition 100 1033–1040.CrossRefGoogle ScholarPubMed
Bloem, M. W., Wedel, M., Egger, R.J., Speek, A.J., Schrijver, J., Saowakontha, S. & Schreurs, W.H.P.(1989).lron metabolism and vitamin deficiency in children in Northeast Thailand. American Journal of Clinical Nutrition 50, 332–338.CrossRefGoogle Scholar
Bloem, M.W., Wedel, M., van Agtmaal, E., Speek, A.J., Saowakontha, S. & Schreurs, W.H.P.(1990).Vitamin A intervention: short-term effects of a single, oral massive dose on iron metabolism. American Journal of Clinical Nutrition 51, 76–79.CrossRefGoogle ScholarPubMed
Chan, V.T. & Wolf, G. (1987). The role of vitamin A in the glycosylation reactions of glycoprotein synthesis in an ‘in vitro’ system. Biochemical Journal 247, 53–62.CrossRefGoogle Scholar
Forbes, A.L., Adams, C.B., Arnaud, M.J., Chichester, C.O., Cook, J.D., Harrison, B.N., Hurrell, R.F., Kahn, S.G., Morris, E.R., Tanner, J. T. &Whittaker, P. (1989). Comparison of in vitro, animal and clinical determinations of iron bioauailability International Nutritional Anemia Consultative Group Task Force report on iron availability. American Journal of Clinical Nutrition 49, 225–238.CrossRefGoogle Scholar
Koessler, K.K., Maurer, S. &Loughlin, R.(1926). The relation of anaemia, primaryand secondary, to vitamin A deficiency.Journal of the American Medical Association 87, 476–482.CrossRefGoogle Scholar
McLaren, D.S., Tchalian, M. &Ajans, Z.A. (1965). Biochemical and hematologic changes in the vitamin A deficient rat. American Journal of Clinical Nutrition 17, 131–138.CrossRefGoogle ScholarPubMed
Mejia, L. A. & Arroyave, G.(1982). The effect of vitamin A fortification of sugar on iron metabolism in preschool children in Guatemala. American Journal of Clinical Nutrition 36, 87–93.CrossRefGoogle ScholarPubMed
Mejie, L.A. &Chew, F. (1988). Hematological effect of supplementing anemic children with vitamin A alone and in combination with iron,. American Journal of Clinical Nutrition 48,595–600.CrossRefGoogle Scholar
Mejia, L. A., Hodges, R. E. & Rucker, R.B. (1979 a). Clinical signs of anaemia in vitamin A-deficient rats. American Journal of Clinical Nutrition 32, 1439–1444.CrossRefGoogle ScholarPubMed
Mejia, L. A., Hodges, R.E. & Rucker, R.B. (1979 b). Role of vitamin A in the absorption, retention and distribution of iron in the rat. Journal of Nutrition 109, 129–137.CrossRefGoogle ScholarPubMed
Mohanram, M., Kulkarni, K. A. & Reddy, V. (1977). Hematological studies in vitamin A deficient children. International Journal of Vitamin and Nutrition Research 47, 389–393.Google ScholarPubMed
Muhilal, Permeisih D., Idjradinata, Y. R., Muherdiyantiningsih, & Karyadi, D. (1988). Vitamin A-fortified monosodium glutamate and health, growth, and survival of children a controlled field trial. American Journal of Clinical Nutrition 48, 1271–1276.CrossRefGoogle ScholarPubMed
National Research Council (1978). Nutrient Requirements of Laboratory Animals. Washington DC: National Academy of Sciences.Google Scholar
Roodenburg, A.J.C., West, C.E., Yu, S. &Beynen, A.C. (1994). Comparison between time-dependent changes in iron metabolism of rats as induced by marginal deficiencyof either vitamin A or iron. British Journal of Nutrition 71, 687–699.CrossRefGoogle ScholarPubMed
Sijtsma, K.W., Van den Berg, G.J., Lemmens, A.G., West, C.E. &Beynen, A.C. (1993).Iron status in rats fed on diets containing marginal amounts of vitamin A. British Journal of Nutrition 70, 777–785.CrossRefGoogle ScholarPubMed
Sklan, D., Harlevy, O. & Donoghue, S. (1986). The effect of different dietary levels of vitamin A on metabolism of copper, iron and zinc in the chick. International Journal for Vitamin and Nutrition Research 51, 11–18.Google Scholar
Snedecor, G.W. & Cochran, W.G.(1980). Statistical Methods, 7th ed., pp.239–240. Ames Iowa State University Press.Google Scholar
Staab, D.B., Hodges, R.E., Metcalf, W.K. & Smith, J.L. (1984). Relationship between vitamin A and iron in the liver. Journal of Nutrition 114, 840–844.CrossRefGoogle ScholarPubMed
Suharno, D., West, C.E., Muhilal, Logman M.H.G.M., de Waart, F. G., Karyadi, D. & Hautvast, J.G.A.J.(1992). Cross sectional study on the iron and vitamin A status of pregnant women in West Java, Indonesia. Ameriean Journal of Clinical Nutrition 56, 988–993.CrossRefGoogle ScholarPubMed
Suhamo, D., West, C.E., Muhilal, Karyadi D. & Hautvast, J. G. A. J.(1993). Not only supplementation with iron but also with vitamin A is necessary to combat nutritional anaemia in pregnant women in West Java, Indonesia. Lancet 342, 1325–1328.Google Scholar
Van Herck, H., Baumans, V., De Boer, S.F., Van Der Gugten, J.,Van Woerkom, A. B. & Beynen, A.C.(1991).Endocrine stress response in rats subjected to singular orbital puncture while under diethyl-ether anaesthesia. Laboratory Animals 25, 325–329.CrossRefGoogle ScholarPubMed
Van Herck, H., Baumans, V., Van Der Craats, N. R., Hesp, A.P.M.,Meijer, G.W., Van Tintelen, G., Walvoort, H.C. & Beynen, A.C.(1992). Histological changes in the orbital region of rats after orbital puncture.Laboratory Animals 26,53–58.CrossRefGoogle ScholarPubMed
Wolde-Gebriel, Z., Haile Gebru Fisseha, T. &West, C.E.(1993 a). Vitamin A, ironand iodine status in children with severe vitamin A deficiency in a rural village in Hararge Region of Ethiopia. European Journal of Clinica1 Nutrition 47, 104–114.Google Scholar
Wolde-Gebriel, Z., West, C.E., Haile Gebru, Tadesse A. S., Fisseha, T., Gabre, P., Ayana, G. & Hautvast, J. G. A. J. (1993 b). Interrelationship between vitamin A, iodine and iron status in schoolchildren in Shoa Region, Central Ethiopia. British Journal of Nutrition 70, 593–607.CrossRefGoogle ScholarPubMed