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Studies on the measurement of 25-hydroxy vitamin D in human saliva

Published online by Cambridge University Press:  09 March 2007

Angela Fairney  and
P. W. Saphier
Angela Fairney
Affiliation:
Department of Chemical Pathology, St. Mary's Hospital Medical School, London W2 1PG
P. W. Saphier
Affiliation:
Department of Chemical Pathology, St. Mary's Hospital Medical School, London W2 1PG
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Abstract

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1. A competitive protein-binding assay for 25-hydroxy vitamin D (25-OHD) in saliva has been established by adaptation of that previously described for 25-OHD in serum (Fairney et al. 1979).

2. Random values of salivary 25-OHD in patients attending hospital for venesection showed a wide range of results (105–1000 pg/ml, n 55). These values corresponded to 1.2% of the total serum values with which they showed a significant relation (r 0.45, P < 0.001).

3. There was no relation between salivary 25-OHD and measured serum free 25-OHD in eighteen pairs of saliva and serum studied.

4. Studies in two individuals showed that salivary 25-OHD values varied throughout the day and that a vitamin D load (19.5 μg), given as pickled herrings at lunch, produced a marked rise in 25-OHD values 5–8 h later.

5. Diurnal profile studies of salivary 25-OHD in Caucasian and Asian 11-year-old male schoolchildren showed lower values in Asian children eating a vegetarian diet, and a significant variation with time and ethnic group(P < 0.001).

6. It is concluded that 25-OHD is present in saliva and that the values vary throughout the day. The values obtained may relate to dietary intake of vitamin D and the subject's ethnic origin.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 57 , Issue 1 , January 1987 , pp. 13 - 25
Copyright
Copyright © The Nutrition Society 1987

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