Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T20:52:12.402Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on the control of gluconeogenesis in sheep: effect of propionate, casein and butyrate infusions

Published online by Cambridge University Press:  24 July 2007

G. J. Judson  and
R. A. Leng
G. J. Judson
Affiliation:
Department of Biochemistry and Nutrition, School of Rural Science, University of New England, Armidale, New South Walves, 2351, Australia
R. A. Leng
Affiliation:
Department of Biochemistry and Nutrition, School of Rural Science, University of New England, Armidale, New South Walves, 2351, Australia
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Short-term effects of infusions of propionate, amino acids and butyrate on gluconeogenesis, as indicated by changes in the irreversible loss of plasma glucose, synthesis of glucose from ruminal propionate or fixation of blood bicarbonate into glucose have been examined in sheep given their daily ration in twenty-four equal portions at hourly intervals.

Sheep received intravenous infusions of [6-3H]glucose usually, in combination with [U-14C]glucose or NaH14CO3 or with intraruminal infusions of [2-14C]propionate. Substrates were infused over a 3–7 h period and followed estimates of pre-infusion kinetic measurements.

2. It was demonstrated that intraruminal and intramesenteric vein infusions of sodium propionate and intra-abomasal infusions of casein hydrolysate stimulated gluconeogenesis. Glucose synthesis showed a linear response to the infusion of these substrates, which varied from 0·35–6·35 mmol propionate/min and 50–160 mg casein/min.

3. The increment in the measured production rate of propionate in the rumen was consistently less than the rate of addition of propionate to the rumen.

4. Intramesenteric vein infusions of sodium butyrate at successive rates of 0·25 and 0·50 mmol/min produced only an initial transient increase in plasma glucose production. Since the rate of glucose synthesis from ruminal propionate was not altered, it was suggested that butyrate initiated glycogen mobilization.

Type
General Nutrition
Information
British Journal of Nutrition , Volume 29 , Issue 2 , March 1973 , pp. 175 - 195
Copyright
Copyright © The Nutrition Society 1973

References

REFERENCES

Annison, E. F., Hill, K. J. & Lewis, D. (1957). Biochem. J. 66, 592.CrossRefGoogle Scholar
Annison, E. F., Leng, R. A., Lindsay, D. B. & White, R. R. (1963). Biochem. J. 88, 248.CrossRefGoogle Scholar
Annison, E. F. & Lindsay, D. B. (1961). Biochem. J. 78, 777.CrossRefGoogle Scholar
Annison, E. F., Lindsay, D. B. & White, R. R. (1963). Biochem. J. 88, 243.CrossRefGoogle Scholar
Annison, E. F. & Linzell, J. L. (1964). J. Physiol., Lond. 175, 372.CrossRefGoogle Scholar
Annison, E. F. & White, R. R. (1961). Biochem. J. 80, 162.CrossRefGoogle Scholar
Ash, R. W., Pennington, R. J. & Reid, R. S. (1964). Biochem. J. 90, 353.CrossRefGoogle Scholar
Ballard, F. J., Hanson, R. W. & Kronfeld, D. S. (1969). Fedn Proc. Fedn Am. Socs exp. Biol. 28, 218.Google Scholar
Bassett, J. M. (1971). Aust. J. biol. Sci. 24, 311.CrossRefGoogle Scholar
Bassett, J. M., Weston, R. H. & Hogan, J. P. (1971). Aust. J. biol. Sci. 24, 321.CrossRefGoogle Scholar
Bergman, E. N. (1963). Am. J. Physiol. 204, 147.CrossRefGoogle Scholar
Bergman, E. N. & Hogue, D. E. (1967). Am. J. Physiol. 213, 1378.CrossRefGoogle Scholar
Bergman, E. N., Roe, W. E. & Kon, K. (1966). Am. J. Physiol. 211, 793.CrossRefGoogle Scholar
Bergman, E. N. & Wolff, J. E. (1971). Am. J. Physiol. 221, 586.CrossRefGoogle Scholar
Black, A. L., Egan, A. R., Anand, R. S. & Chapman, T. E. (1968). In Isotope Studies on the Nitrogen Chain p. 247. Vienna: I. A. E. A.Google Scholar
Black, A. L., Luick, J., Moller, F. & Anand, R. S. (1966). J. biol. Chem. 241, 5233.CrossRefGoogle Scholar
Blaxter, K. L. & Martin, A. K. (1962). Br. J. Nutr. 16, 397.CrossRefGoogle Scholar
Cook, R. M. & Miller, L. D. (1965). J. Dairy Sci. 48, 1339.CrossRefGoogle Scholar
Corbett, J. L., Farrell, D. J., Leng, R. A., McClymont, G. L. & Young, B. A. (1971). Br. J. Nutr. 26, 277.CrossRefGoogle Scholar
Depocas, F. & De Freitas, A. S. W. (1970). Can. J. Physiol. Pharmac. 48, 557.CrossRefGoogle Scholar
El-Shazly, K. & Hungate, R. E. (1965). Appl. Microbiol. 13, 62.CrossRefGoogle Scholar
Exton, J. H., Jefferson, L. S., Butcher, R. W. & Park, C. R. (1966). Am. J. Med. 40, 709.CrossRefGoogle Scholar
Exton, J. H., Mallette, L. E., Jefferson, L. S., Wong, E. H. A., Friedmann, N., Miller, T. B. & Park, C. R. (1970). Recent Prog. Hornt. Res. 26, 411.Google Scholar
Ford, E. J. H. (1963). Biochem. J. 88, 427.CrossRefGoogle Scholar
Ford, E. J. H. (1965). J. agric. Sci., Camb. 65, 41.CrossRefGoogle Scholar
Gray, F. V., Weller, R. A., Pilgrim, A. F. & Jones, G. B. (1966). Aust. J. agric. Res. 17, 69.CrossRefGoogle Scholar
Halfpenny, A. F., Rook, J. A. F. & Smith, G. B. (1969). Br. J. Nutr. 23, 547.CrossRefGoogle Scholar
Hobson, P. N. (1965). J. gen. Microbiol. 38, 167.CrossRefGoogle Scholar
Hobson, P. N. & Summers, R. (1967). J. gen. Microbiol. 47, 53.CrossRefGoogle Scholar
Horino, M., Machlin, L. J., Hertelendy, F. & Kipnis, D. M. (1968). Endocrinology 83, 118.CrossRefGoogle Scholar
Jarrett, I. G., Potter, B. J. & Filsell, O. H. (1952). Aust. J. exp. Biol. med. Sci. 30, 197.CrossRefGoogle Scholar
Judson, G. J., Anderson, E., Luick, J. R. & Leng, R. A. (1968). Br. J. Nutr. 22, 69.CrossRefGoogle Scholar
Judson, G. J. & Leng, R. A. (1968). Proc. Aust. Soc. Anim. Prod. 7, 354.Google Scholar
Judson, G. J. & Leng, R. A. (1973). Br. J. Nutr. 29, 159.CrossRefGoogle Scholar
Katz, M. L. & Bergman, E. N. (1969). Am. J. Physiol. 216, 953.CrossRefGoogle Scholar
Kaufman, C. F. & Bergman, E. N. (1971). Am. J. Physiol. 221, 967.CrossRefGoogle Scholar
Keech, D. B. & Utter, M. F. (1963). J. biol. Chem. 238, 2609.CrossRefGoogle Scholar
Krebs, H. A. (1964). In Mammalian Protein Metabolism p. 125 [Munro, H. N. and Allison, J. B., editors]. New York: Academic Press.CrossRefGoogle Scholar
Krebs, H. A. & Yoshida, T. (1963). Biochem. J. 89, 398.CrossRefGoogle Scholar
Kronfeld, D. S. & Simesen, M. G. (1961). Am. J. Physiol. 201, 639.CrossRefGoogle Scholar
Leng, R. A. (1970 a). Adv. vet. Sci. 14, 209.Google Scholar
Leng, R. A. (1970 b). In Physiology of Digestion and Metabolism in the Ruminant p. 406 [Phillipson, A. T., editor]. Newcastle upon Tyne: Oriel Press Ltd.Google Scholar
Leng, R. A. & Annison, E. F. (1963). Biochem. J. 86, 319.CrossRefGoogle Scholar
Leng, R. A., Steel, J. W. & Luick, J. R. (1967). Biochem. J. 103, 785.CrossRefGoogle Scholar
Lindsay, D. B. & Williams, K. L. (1971). Proc. Nutr. Soc. 30, 35A.CrossRefGoogle Scholar
Manns, J. G. (1969). Fedn Proc. Fedn Am. Socs exp. Biol. 28, 491.Google Scholar
Manns, J. G. & Boda, J. M. (1967). Am. J. Physiol. 212, 747.CrossRefGoogle Scholar
Manns, J. G., Boda, J. M. & Willes, R. F. (1967). Am. J. Physiol. 212, 756.CrossRefGoogle Scholar
Moodie, E. W., Walker, A. I. T. & Hutton, P. H. (1963). Q. Jl exp. Physiol. 48, 379.CrossRefGoogle Scholar
Ohneda, A., Parada, E., Eisentraut, A. M. & Unger, R. H. (1968). J. clin. Invest. 47, 2305.CrossRefGoogle Scholar
Pennington, R. J. & Sutherland, T. M. (1956). Biochem. J. 63, 618.CrossRefGoogle Scholar
Phillips, R. W. & Black, A. L. (1966). J. comp. Biochem. Physiol. 18, 527.CrossRefGoogle Scholar
Phillips, R. W., Black, A. L. & Moller, F. (1965). Life Sciences 4, 521.CrossRefGoogle Scholar
Phillips, R. W., House, W. A., Miller, K. A., Mott, J. L. & Sooby, D. L. (1969). Am. J. Physiol. 217, 1265.CrossRefGoogle Scholar
Potter, E. L., Purser, D. B. & Cline, J. H. (1968). J. Nutr. 95, 655.CrossRefGoogle Scholar
Reilly, P. E. B. & Ford, B. J. H. (1971). Br. J. Nutr. 26, 249.CrossRefGoogle Scholar
Reis, P. J. & Schinckel, P. G. (1961). Aust. J. agric. Res. 12, 335.CrossRefGoogle Scholar
Rosen, H. (1957). Archs Biochem. Biophys. 67, 10.CrossRefGoogle Scholar
Smith, R. M. & Marston, H. R. (1971). Br. J. Nutr. 26, 41.CrossRefGoogle Scholar
Steel, J. W. & Leng, R. A. (1968). Proc. Aust. Soc. Anim. Prod. 7, 342.Google Scholar
Taylor, T. A. & Ramsay, H. A. (1965). J. Dairy Sci. 48, 505.CrossRefGoogle Scholar
Walker, D. J. (1965). In Physiology of Digestion in the Ruminant p. 296 [Dougherty, R. W., editor]. Washington, DC: Butterworths.Google Scholar
Weidermann, M. J. & Krebs, H. A. (1969). Biochem. J. 112, 149.CrossRefGoogle Scholar
Williamson, D. H., Mellanby, J. & Krebs, H. A. (1962). Biochem. J. 82, 90.CrossRefGoogle Scholar
Young, J. W., Thorp, S. L. & De Lumen, H. Z. (1969). Biochem. J. 114, 83.CrossRefGoogle Scholar