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Soya phytonutrients act on a panel of genes implicated with BRCA1and BRCA2 oncosuppressors in human breast cell lines

Published online by Cambridge University Press:  08 March 2007

Bertrand Caëtano ,
Ludovic Le Corre ,
Nassera Chalabi ,
Laetitia Delort ,
Yves-Jean Bignon  and
Dominique J. Bernard-Gallon
Bertrand Caëtano
Affiliation:
Département d'Oncogénétique du Centre Jean PerrinINSERM UMR 484-UdA, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P.38, 63 001 Clermont-Ferrand Cedex 01, France
Ludovic Le Corre
Affiliation:
Département d'Oncogénétique du Centre Jean PerrinINSERM UMR 484-UdA, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P.38, 63 001 Clermont-Ferrand Cedex 01, France
Nassera Chalabi
Affiliation:
Département d'Oncogénétique du Centre Jean PerrinINSERM UMR 484-UdA, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P.38, 63 001 Clermont-Ferrand Cedex 01, France
Laetitia Delort
Affiliation:
Département d'Oncogénétique du Centre Jean PerrinINSERM UMR 484-UdA, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P.38, 63 001 Clermont-Ferrand Cedex 01, France
Yves-Jean Bignon*
Affiliation:
Département d'Oncogénétique du Centre Jean PerrinINSERM UMR 484-UdA, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P.38, 63 001 Clermont-Ferrand Cedex 01, France
Dominique J. Bernard-Gallon
Affiliation:
Département d'Oncogénétique du Centre Jean PerrinINSERM UMR 484-UdA, Centre Biomédical de Recherche et de Valorisation, 28 place Henri Dunant, B.P.38, 63 001 Clermont-Ferrand Cedex 01, France
*
*corresponding author: Professor Yves-Jean Bignon, fax +33 4 73 27 80 42, email [email protected]
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Abstract

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Breast cancer is the most common cancer in women and a significant cause of death. Mutations of the oncosuppressor genes BRCA1 and BRCA2 are associated with a hereditary risk of breast cancer, and dysregulation of their expression has been observed in sporadic cases. Soya isoflavones have been shown to inhibit breast cancer in studies in vitro, but associations between the consumption of isoflavone-containing foods and breast cancer risk have varied in epidemiological studies. Soya is a unique source of the phytoestrogens daidzein (4′,7-dihydroxyisoflavone) and genistein (4′,5,7-trihydroxyisoflavone), two molecules that are able to inhibit the proliferation of human breast cancer cells in vitro. The aim of the present study was to determine the effects of genistein (5μg/ml) and daidzein (20μg/ml) on transcription in three human breast cell lines (one dystrophic, MCF10a, and two malignant, MCF-7 and MDA-MB-231) after 72h treatment. The different genes involved in the BRCA1 and BRCA2 pathways (GADD45A, BARD1, JUN, BAX, RB1, ERα, ERβ, BAP1, TNFα, p53, p21Waf1/Cip1, p300, RAD51, pS2, Ki-67) were quantified by real-time quantitative RT-PCR, using the TaqMan method and an ABI Prism 7700 Sequence Detector (Applied Biosystems). We observed that, in response to treatment, many of these genes were overexpressed in the breast cancer cell lines (MCF-7 and MDA-MB-231) but not in the dystrophic cell line (MCF10a).

Keywords

GenisteinDaidzeinBRCA1BRCA2Real-time quantitative RT-PCR
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 406 - 413
Copyright
Copyright © The Nutrition Society 2006

References

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