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Simultaneous determination of absorption of selenium from poultry meat and selenite in young men: application of a triple stable-isotope method

Published online by Cambridge University Press:  09 March 2007

Merrill J. Christensen ,
Morteza Janghorbani ,
Fred H. Steinke ,
Nawfal Istfan  and
Vernon R. Young
Merrill J. Christensen
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, 138 Albany Street, Cambridge, Massachusetts 02139, USA
Morteza Janghorbani
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, 138 Albany Street, Cambridge, Massachusetts 02139, USA Nuclear Reactor Laboratory, Massachusetts Institute of Technology, 138 Albany Street, Cambridge, Massachusetts 02139, USA
Fred H. Steinke
Affiliation:
Central Research, Ralston Purina Company, St Louis, Missouri 63188, USA
Nawfal Istfan
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, 138 Albany Street, Cambridge, Massachusetts 02139, USA
Vernon R. Young
Affiliation:
Department of Nutrition and Food Science, Massachusetts Institute of Technology, 138 Albany Street, Cambridge, Massachusetts 02139, USA
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Abstract

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1. A triple stable-isotope method was used to estimat simultaneously selenium absorption from poultry meat intrinsically labelled with 74Se and from an extrinsic tracer of 76SeO32− in four young adult men. The subjects received an experimental diet based on chicken meat as the source of protein. Two diet periods, lasting from 5 to 10 d, depending on the frequency of stool output for each subject, were conducted to allow duplicate determinations within each subject of Se absorption from both sources.

2. Absorption of Se was determined by the faecal isotope balance procedure through monitoring the stable isotopes 74Se, 76Se and 80Se by means of radiochemical neutron activation analysis.

3. For the separate periods, mean (with SEM) absorption of 74Se from the intrinsically-labelled poultry meat was 70·9 (1·5)% and 72·0 (2·5)%. The absorption of Se from 76SeO32− was 34·7 (5·5)% and 37·6 (3·2)% during the same periods.

4. From these results it is concluded that, under the present experimental conditions, the intrinsic and extrinsic Se isotopes did not form a common pool before absorption. Possible reasons for this are discussed.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 50 , Issue 1 , July 1983 , pp. 43 - 50
Copyright
Copyright © The Nutrition Society 1983

References

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