Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-23T20:37:41.548Z Has data issue: false hasContentIssue false
  • English
  • Français

The similarity between alkaline phosphatase (EC 3.1.3.1) and phytase (EC 3.1.3.8) activities in rat intestine and their importance in phytate-induced zinc deficiency

Published online by Cambridge University Press:  24 July 2007

N. T. Davies  and
A. A. Flett
N. T. Davies
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
A. A. Flett
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. The activities of alkaline phosphatase (EC 3.1.3.1) and phytase (EC3.1.3.8) were similarly distributed in the small intestine of rats. Regional differences in activity were reflected by similar differences in the capacity of ligated intestinal segments to hydrolyse phytate in vivo. Activities were greatest in the duodenum and lowest in the terminal ileum.

2. Specific activities of both enzymes were tenfold greater in the brush border fraction of duodenal mucosa compared with entire mucosal homogenates.

3. Brush-border alkaline phosphatase and phytase activities required both magnesium and zinc ions for maximal activity.

4. Zn deficiency induced by feeding a diet low in Zn (0.5 mg Zn/kg) caused similar reductions in activity of both enzymes.

5. Zn deficiency induced by feeding diets marginally adequate in Zn (12 mg/kg) and phytate (10g/kg) caused reductions in alkaline phosphatase, phytase activities and phytate hydrolysis in vivo.

6. It is suggested that phytase activity is a manifestation of alkaline phosphatase and the significance of this in relation to phytate-induced Zn deficiency is discussed.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 39 , Issue 2 , March 1978 , pp. 307 - 316
Copyright
Copyright © The Nutrition Society 1978

References

REFERENCES

Bitar, K. & Reinhold, J. G. (1972). Biochim. biophys. Acta 268, 442.CrossRefGoogle Scholar
Bruce, H. M. & Callow, R. K. (1934). Biochem. J. 28, 517.CrossRefGoogle Scholar
Chesters, J. K. & Will, M. (1973). Br. J. Nutr. 30, 555.CrossRefGoogle Scholar
Davies, M. & Motzok, I. (1972). Comp. Biochem. Physiol. 42, 345.Google Scholar
Davies, M., Ritcey, G. M. & Motzok, I. (1970). Poult. Sci. 49, 1280.CrossRefGoogle Scholar
Davies, N. T. & Nightingale, R. (1975). Br. J. Nutr. 34, 243.CrossRefGoogle Scholar
Holt, R. (1955). J. Sci. Fd Agric. 6, 136.CrossRefGoogle Scholar
Hubscher, G., West, G. R. & Brindley, D. N. (1965). Biochem. J. 97, 629.CrossRefGoogle Scholar
Leucke, R. W., Olman, M. E. & Baltzer, B. V. (1968). J. Nutr. 94, 344.CrossRefGoogle Scholar
Maddaiah, V. T., Kurnick, A. A., Hulett, B. J. & Reid, B. L. (1969). Proc. Soc. exp. Biol. Med. 115, 1054.CrossRefGoogle Scholar
Miller, D. & Crane, R. K. (1961). Biochim. biophys. Acta 52, 293.CrossRefGoogle Scholar
Miller, G. L. (1959). Analyt. Chem. 31, 964.CrossRefGoogle Scholar
Moog, F. (1968). In Handbook of Physiology: Alimentary Canal Vol. 3. Intestinal absorption. Washington, DC: American Physiological Society.Google Scholar
Oberleas, D. (1973). Toxicant Occurring Naturally in Foods, p. 363. Washington, DC: National Academy of Sciences.Google Scholar
Oberleas, D. (1975). In Proceedings Western Hemisphere Nutrition Congress IV 1975, p. 156 [White, P. L., Selvey, N., editors]. Acton, Massachusetts: Publishing Sciences Group, Inc.Google Scholar
O'Dell, B. L. & Savage, J. E. (1957). Fedn Proc. Fedn Am. Socs. exp. Biol. 16, 394.Google Scholar
Patwardhen, V. N. (1937). Biochem. J. 31, 560.CrossRefGoogle Scholar
Pileggi, V. J. (1959). Archs. Biochem. Biophys. 30, 1.CrossRefGoogle Scholar
Reinhold, J. G., Pascoe, E., Arslanian, M. & Bitar, K. (1970). Biochim. biophys. Acta 215, 430.CrossRefGoogle Scholar
Seelig, M. S. (1964). Am. J. clin. Nutr. 14, 342.CrossRefGoogle Scholar
Sumner, J. B. (1944). Science, N. Y. 160, 413.CrossRefGoogle Scholar
Widdowson, E. M. & McCance, R. A. (1942). Lancet i, 588.CrossRefGoogle Scholar
Williams, R. B. (1972). Br. J. Nutr. 27, 121.CrossRefGoogle Scholar
Williams, R. B. & Mills, C. F. (1970). Br. J. Nutr. 24, 989.CrossRefGoogle Scholar