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Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats

Published online by Cambridge University Press:  08 March 2007

Marta Miguel ,
Rosina López-Fandiño ,
Mercedes Ramos  and
Amaya Aleixandre
Marta Miguel
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
Rosina López-Fandiño
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
Mercedes Ramos
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
Amaya Aleixandre*
Affiliation:
Instituto de Farmacología y Toxicología (CSIC), Departamento de Farmacología, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain
*
*Corresponding author: Dr Amaya Aleixandre, fax +34 91 3941463, email [email protected]
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Abstract

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In the present study we evaluate the blood pressure-lowering effect of the following products: the hydrolysate obtained from egg white (EW) by enzymatic treatment with pepsin (HEW), the peptide fraction of HEW with molecular mass lower than 3000 Da (HEW<3000 Da), and three peptide sequences isolated from HEW<3000 Da (Tyr-Ala-Glu-Glu-Arg-Tyr-Pro-Ile-Leu: YAEERYPIL); (Arg-Ala-Asp-His-Pro-Phe-Leu: RADHPFL); and (Ile-Val-Phe (IVF)). These peptides, and also HEW and HEW<3000 Da, had been characterized previously in vitro as potent inhibitors of angiotensin-converting enzyme (ACE). EW and the products mentioned earlier were orally administered by gastric intubation, to 17–20-week-old male spontaneously hypertensive rats (SHR) and normotensive Wistar–Kyoto (WKY) rats. We measured the systolic blood pressure (SBP) and the diastolic blood pressure (DBP) of the rats by the tail cuff method before administration and also 2, 4, 6, 8 and 24h post-administration. Distilled water served as negative control, and we used captopril (50mg/kg) as positive control to carry out similar experiments with a known ACE inhibitor. HEW, HEW<3000 Da and the three peptide sequences decreased SBP and DBP in SHR but they did not modify these variables in WKY rats. The peptide sequences YAEERYPIL, RADHPFL and IVF showed a potency to decrease blood pressure greater than HEW or HEW<3000 Da. The results obtained suggest that the studied products could be used as a functional food with potential therapeutic benefit in the prevention and treatment of hypertension.

Keywords

HypertensionAngiotensin-converting enzymeBioactive peptidesSpontaneously hypertensive rats
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 731 - 737
Copyright
Copyright © The Nutrition Society 2005

References

Akpaffiong, MJ & Taylor, AA (1998) Antihypertensive and vasodilator actions of antioxidants in spontaneously hypertensive rats. Am J Hypertens 11, 1450–1460.CrossRefGoogle ScholarPubMed
Atherton, E & Sheppard, RC (1989) Solid phase synthesis. A practical approach. Oxford: IRL Press.Google Scholar
Bazil, MK, Krulan, C & Webb, RL (1993) Telemetric monitoring of cardiovascular parameters in conscious spontaneously hypertensive rats. J Cardiovasc Pharmacol 22, 897–905.CrossRefGoogle ScholarPubMed
Buñag, RD (1973) Validation in awake rats of a tail-cuff method for measuring systolic pressure. J Appl Physiol 34, 279–282.CrossRefGoogle ScholarPubMed
Chen, TL, Lo, YC, Hu, WT, Wu, MC, Chen, ST & Chang, HM (2003) Microencapsulation and modification of synthetic peptides of food proteins reduces the blood pressure of spontaneously hypertensive rats. J Agric Food Chem 51, 1671–1675.CrossRefGoogle ScholarPubMed
Dávalos, A, Miguel, M, Bartolomé, B & López-Fandiño, R (2004) Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Protect 67, 1939–1944.CrossRefGoogle ScholarPubMed
Fujita, H, Sasaki, R & Yoshikawa, M (1995a) Potentiation of the antihypertensive activity of orally administered Ovokinin, a vasorelaxing peptide derived from ovalbumin, by emulsification in egg phosphatidyl-choline. Biosci Biotechnol Biochem 59, 2344–2345.CrossRefGoogle Scholar
Fujita, H, Usui, H, Kurahashi, K & Yoshikawa, M (1995b) Isolation and characterization of ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin. Peptides 16, 785–790.CrossRefGoogle ScholarPubMed
Fujita, H, Yamagami, T & Ohshima, K (2001) Effects of an ACE-inhibitory agent, katsuobushi oligopeptide, in the spontaneously hypertensive rat and in borderline and mildly hypertensive subjects. Nutr Res 21, 1149–1158.CrossRefGoogle Scholar
Fujita, H & Yoshikawa, M (1999) LKPNM:a prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology 44, 123–127.CrossRefGoogle ScholarPubMed
Gómez-Ruiz, JA, Ramos, M & Recio, I (2003) Angiotensin-converting enzyme-inhibitory peptides in Manchego cheeses manufactured with diferent starter cultures. Int Dairy J 12, 697–706.CrossRefGoogle Scholar
Karaki, H, Doi, K, Sugano, S, Uchiwa, H, Sugai, R, Murakami, U & Takemoto, S (1990) Antihypertensive effect of tryptic hydrolysate of milk casein in spontaneously hypertensive rats. Comp Biochem Physiol 96, 367–371.Google ScholarPubMed
Kawasaki, T, Jun, CJ, Fukushina, Y, Kegai, K, Seki, E, Osajima, K, Itoh, K, Matsui, T & Matsumoto, K (2002) Antihypertensive effect and safety evaluation of vegetable drink with peptides derived from sardine protein hydrolysated on mild hypertensive, high normal, and normal blood pressure subjects. Fukuoka Igaku Zasshi 93, 208–218.Google Scholar
Leclerc, PL, Gauthier, SF, Bachelard, H, Santure, M & Roy, D (2002) Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus. Int Dairy J 12, 995–1004.CrossRefGoogle Scholar
Matoba, N, Usui, H, Fujita, H & Yoshikawa, M (1999) A novel antihypertensive peptide derived from ovalbumin induces nitric oxide-mediated vasorelaxation in an isolated SHR mesenteric artery. FEBS Lett 452, 181–184.CrossRefGoogle Scholar
Matoba, N, Yamada, Y, Usui, H, Nakagiri, R & Yoshikawa, M (2001) Designing potent derivatives of Ovokinin(2–7), an anti-hypertensive peptide derived from ovalbumin. Biosci Biotechnol Biochem 65, 636–739.CrossRefGoogle ScholarPubMed
Miguel, M, Muguerza, B, Sánchez, E, Delgado, MA, Recio, I, Ramos, M & Aleixandre, MA (2005) Changes in arterial blood pressure in hypertensive rats caused by long-term intake of milk fermented by Enterococcus faecalis CECT 572. Br J Nutr 94, 36–43.CrossRefGoogle Scholar
Miguel, M, Recio, I, Gomez-Ruiz, JA, Ramos, M & Lopez-Fandiño, R (2004) Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Protect 67, 1914–1920.CrossRefGoogle ScholarPubMed
Mizushima, S, Ohshige, K, Watanabe, J, Kimura, M, Kadowaki, T, Nakamura, Y, Tochikubo, O & Ueshima, H (2004) Randomized controlled trial of sour milk on blood pressure in borderline hypertensive men. Am J Hypertens 17, 701–706.CrossRefGoogle ScholarPubMed
Nakamura, Y, Masuda, OY & Takano, T (1996) Decrease of tissue angiotensin I-converting enzyme activity upon feed sour milk in spontaneously hypertensive rats. Biosci Biotechnol Biochem 60, 488–489.CrossRefGoogle Scholar
Nakamura, Y, Yamamoto, N, Sakai, KY & Takano, T (1995) Antihypertensive effect of sour milk and peptides isolated from it that are inhibitors to angiotensin I converting enzyme. J Dairy Sci 78, 1253–1257.CrossRefGoogle ScholarPubMed
Nurminen, ML, Sipola, M, Kaarto, H, Pihlanto-Leppälä, A, Piilola, K, Korpela, R, Tossavainen, H, Korhonen, H & Vapaatalo, H (2000) α-Lactorphin lowers blood pressure measured by radiotelemetry in normotensive and spontaneously hypertensive rats. Life Sci 16, 1535–1543.CrossRefGoogle Scholar
Okamoto, K & Aoki, K (1963) Development of a strain of spontaneously hypertensive rats. Jpn Circ J 27, 282–293.CrossRefGoogle ScholarPubMed
Rodríguez-Iturbe, B, Zhan, CD, Quiroz, Y, Sindhu, RF & Vaziri, ND (2003) Antioxidant-rich diet relieves hypertension and reduces renal immune infiltration in spontaneously hypertensive rats. Hypertension 41, 341.CrossRefGoogle ScholarPubMed
Saito, Y, Wanezaki, K, Kawato, A & Imayasu, S (1994) Antihypertensive effects of peptide in sake and its by-products on spontaneously hypertensive rats. Biosci Biotechnol Biochem 58, 812–816.CrossRefGoogle ScholarPubMed
Seppo, L, Jauhiainen, T, Poussa, T & Korpela, R (2003) A fermented milk high in bioactive peptides has a blood pressure-lowering effect in hypertensive subjects. Am J Clin Nutr 77, 326–330.CrossRefGoogle Scholar
Sipola, M, Finckenberg, P, Korpela, R, Vapaatalo, H & Nurminen, ML (2002) Effect of long-term intake of milk products on blood pressure in hypertensive rats. J Dairy Res 69, 103–111.CrossRefGoogle ScholarPubMed
Soares de Moura, R, Costa Viana, FS, Souza, MAV, Kovary, K, Guedes, DC, Oliveira, EPB, Rubenich, LSM, Carvalho, LC, Oliveira, RM, Tano, RM, Gusmão, T & Correia, ML (2002) Antihypertensive, vasodilator and antioxidant effects of a vinifiera grape skin extract. J Pharm Pharmacol 54, 515–1520.Google Scholar
Wright, GL & Rankin, GO (1982) Concentrations of ionic and total calcium in plasma of four models of hypertension. Am J Physiol 242, H365–H370.Google Scholar
Wu, J & Ding, X (2001) Hypotensive and physiological effect of angiotensin converting enzyme inhibitory peptides derived from soy protein on spontaneously hypertensive rats. J Agric Food Chem 49, 501–506.CrossRefGoogle ScholarPubMed
Yamada, Y, Matoba, N, Usui, H, Onishi, K & Yoshikawa, M (2002) Design of a highly potent anti-hypertensive peptide based on Ovokinin(2–7). Biosci Biotechnol Biochem 66, 1213–1217.CrossRefGoogle ScholarPubMed
Zicha, J & Kunes, J (1999) Ontogenetic aspects of hypertension development: analysis in the rat. Physiol Rev 79, 1227–1282.CrossRefGoogle ScholarPubMed