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Serum protein fractions from children of differing nutritional status analysed by polyacrylamide gel electrophoresis and electroimmunoassay

Published online by Cambridge University Press:  25 March 2008

F. P. Schelp ,
P. Migasena ,
S. Saovakontha ,
Praneet Pongpaew  and
Venus Supawan
F. P. Schelp
Affiliation:
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
P. Migasena
Affiliation:
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
S. Saovakontha
Affiliation:
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Praneet Pongpaew
Affiliation:
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Venus Supawan
Affiliation:
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Abstract

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1. The nutritional status of children showing no clinical signs of malnutrition, from the University School of Khon Kaen, Khon Kaen province, north-east Thailand and from two villages nearby, was tested. The children were grouped according to their body-weight expressed as a percentage of expected weight-for-height (Harvard standards (Stuart & Stevenson, 1959), as given by Jelliffe (1966))..

2. The differing prealbumin concentrations indicated that nutritional status differed between the groups..

3. The urinary urea: creatinine ratio was significantly lower in the village children compared with the children from Khon Kaen, indicative of the higher dietary protein intake of the latter..

4. α1-Acid glycoprotein and the first ‘post-albumin peak’ (obtained by polyacrylamide gel electrophoresis of serum and containing mainly Gc-globulin, α1-antichymotrypsin and α1-B-glycoprotein) were found to be significantly higher in the village children compared with children from Khon Kaen..

5. The three main proteins of the first ‘post-albumin peak’ from polyacrylamide gel electrophoresis of serum were tested separately using the electroimmunoassay method. There was no significant difference in Gc-globulin between the children from Khon Kaen and the village children. The concentration of α1-B-glycoprotein from those Khon Kaen children whose body-weight was more than 95% expected weight-for-height was significantly lower compared with that of village children. α1-Antichymotrypsin concentration was significantly higher in serum from Khon Kaen children than in serum from village children.

Type
Papers of direct relevance to Clinical and Human Nutrition
Information
British Journal of Nutrition , Volume 35 , Issue 2 , March 1976 , pp. 211 - 222
Copyright
Copyright © The Nutrition Society 1976

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