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Secretion and reabsorption of endogenous protein along the small intestine of sheep: estimates derived from 15N dilution of plasma non-protein-N

Published online by Cambridge University Press:  09 March 2007

J. Van Bruchem ,
J Voight ,
Toos S. C. W Lammers-Wienhoven ,
Ulrike Schönhusen ,
J. J. M. H Ketelaars  and
S Tamminga
J. Van Bruchem
Affiliation:
Wageningen Institute of Animal Sciences (WIAS), Agricultural University, Haarweg 10, 6709 PJ Wageningen, The Netherlands
J Voight
Affiliation:
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Fachbereich Ernährungsphysiologie ‘Oskar Kellner’, Rostock, Germany
Toos S. C. W Lammers-Wienhoven
Affiliation:
Wageningen Institute of Animal Sciences (WIAS), Agricultural University, Haarweg 10, 6709 PJ Wageningen, The Netherlands
Ulrike Schönhusen
Affiliation:
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Fachbereich Ernährungsphysiologie ‘Oskar Kellner’, Rostock, Germany
J. J. M. H Ketelaars
Affiliation:
DLO-Research Institutefor Agrobiology and Soil Fertility (AB-DLO), Wageningen, The Netherlands
S Tamminga
Affiliation:
Wageningen Institute of Animal Sciences (WIAS), Agricultural University, Haarweg 10, 6709 PJ Wageningen, The Netherlands
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Abstract

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Sheep fitted with a PVC cannula in the dorsal rumen, a silastic infusion tube in the abomasum, and single ‘T’-shape PVC cannulas in the proximal duodenum (D1), distal duodenum (D2), mid-jejunum (J), and terminal ileum (I), were fed on diets varying in crude protein (Nx6·25) and fibre contents. 15N was administered to the sheep as a 15N-labelled grass meal-beer yeast suspension which was continuously infused into the abomasum over a period of 1 week. Subsequently, over a period of 4 d, 15N excess in the D1, D2, J and I digesta and faeces was measured and related to 15N excess in blood plasma TCA-soluble N. Average daily intakes of DM and N were 1·12 kg and 26·3 g respectively. Average daily endogenous N (g) amounted to 3·1 at D1, 10·2 at D2, 6·1 at J, 5·0 at I, and 5·1 in the faeces (F). Corresponding percentage values for total N were: D1 12·4, D2 32·9, J 46-4, I 48.4 and F 50.7. The secretion, particularly of pancreatic juice and/or bile, varied. The apparent re-absorption of endogenous protein varied among experimental diets, on average by 50·4% between D2 and I. Along the small intestine the percentage of 15N in the TCA-precipitable fraction gradually changed, on average by 66·0, 63·0, 46·3 and 55·7 at D1, D2, J and I respectively. Because of differential rates of enrichment and turnover among protein pools which contribute towards endogenous secretion, care should be taken in interpreting these data as absolute values.

Keywords

Endogenous proteinProtein conversionEnvironmental N burden
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 2 , February 1997 , pp. 273 - 286
Copyright
Copyright © The Nutrition Society 1997

References

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