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Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids

Published online by Cambridge University Press:  09 March 2007

María A. Navarro ,
Sergio Acín ,
Ricardo Carnicer ,
Mario A. Guzmán-García ,
José M. Arbonés-Mainar ,
Joaquín C. Surra ,
José A. Cebrián ,
Carmen Arnal ,
Beatriz Isabel  and
Clemente J. López-Bote
...Show all authors
María A. Navarro
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
Sergio Acín
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
Ricardo Carnicer
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
Mario A. Guzmán-García
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
José M. Arbonés-Mainar
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
Joaquín C. Surra
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
José A. Cebrián
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
Carmen Arnal
Affiliation:
Departamento de Patología Animal, Universidad de Zaragoza, Spain
Beatriz Isabel
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
Clemente J. López-Bote
Affiliation:
Departamento de Producción Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain
Jesús Osada*
Affiliation:
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Spain
*
*Corresponding author: Dr J. Osada, fax +34 976 761 612, email, [email protected]
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Abstract

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ApoA-IV is a protein constituent of HDL particles; the gene coding for it is a member of the ApoA-I–ApoC-III–ApoA-IV cluster. To investigate the effects of the quantity and the degree of saturation of dietary lipid on the long-term response of this Apo, and on the hypothetical coordinated regulation of the cluster in vivo, pigs were fed isoenergetic, cholesterol-free, low-lipid or lipid-enriched diets (containing either extra olive oil (rich in MUFA) or sunflower oil (rich in n−6 PUFA)) for 42 d. In animals fed on the control diet, ApoA-IV was mainly associated with plasma lipoproteins. An increase in plasma ApoA-IV concentration, mainly in the lipoprotein-free fraction, was induced by the lipid-enriched diets, independent of the degree of saturation of the fatty acids involved. The latter diets also led to increases in hepatic ApoA-I, ApoA-IV and ApoC-III mRNA levels, more so with the sunflower oil-rich diet. The present results show that porcine plasma ApoA-IV levels and their association with lipoproteins are very sensitive to increases in dietary lipids, independent of the degree of fatty acid saturation. Furthermore, hepatic expression of RNA appears to be coordinated along with that of the other members of the gene cluster.

Keywords

Apolipoprotein A-IVDietary fatPig
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 5 , November 2004 , pp. 763 - 769
Copyright
Copyright © The Nutrition Society 2004

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