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The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages

Published online by Cambridge University Press:  09 March 2007

M. Blümmel ,
H. Steingaβ  and
K. Becker
M. Blümmel
Affiliation:
Institute for Animal Production in the Tropics and Subtropics. University of Hohenheim (480), D-70 593 Stuttgart, Germany
H. Steingaβ
Affiliation:
Institute for Animal Nutrition, University of Hohenheim (450), D-70 593 Stuttgart, Germany
K. Becker
Affiliation:
Institute for Animal Production in the Tropics and Subtropics. University of Hohenheim (480), D-70 593 Stuttgart, Germany
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Abstract

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The relationship between in vitro gas production, concomitant in vitro apparent and true DM degradability has been examined in forty-two roughages. The partitioning of truly-degraded substrate between gas volume and microbial biomass yield and 15N incorporation into cells was also investigated. The relevance of this partitioning for the regulation of DM intake (DMI) was examined for fifty-four roughages. The results can be summarized as follows. In vitro gas production and in vitro apparent and true degradability are highly correlated (P<0.0001), r being 0.96 and 0.95 respectively. There is an inverse relationship between in vitro gas production and microbial biomass yield (r—0.67, (P<0.0001) and also 15N enrichment (P<0.001)when the variables were related to a given unit of substrate truly degraded. Selecting roughages by in vitro gas production may well be a selection against maximum microbial yield and a combination of in vitro gas volume measurements with a complementary determination of the substrate truly degraded is proposed, to calculate a partitioning factor (PF) reflecting the variation of short-chain fatty acid production per unit substrate degraded. PF is calculated as the ratio, substrate truly degraded: gas produced by it. PF was highly significant (P<0.0001) in DMI prediction when included in stepwise multiple correlations together with in vitro gas volume variables reflecting the extent and rate of gas production; 11 % of the variation in DMI was accounted for by the PF. The total model, including extent and rate of gas production and the PF, accounted for 84 % of the variation in DMI. Roughages producing proportionally less gas per unit substrate truly degraded had higher feed intakes.

Keywords

Gas productionMicrobial activity15N incorporationIn vitro degradabilityVoluntary feed intake
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 6 , June 1997 , pp. 911 - 921
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Bas, F. J., Stern, M. D. & Merchen, N. R. (1989). Influence of protein supplementation of alkaline hydrogen peroxide-treated wheat straw on ruminal microbial fermentation. Journal of Dairy Science 72, 1217–1227.CrossRefGoogle ScholarPubMed
Beuvink, J. M. W. & Kogut, J. (1993). Modelling gas production kinetics of grass silages incubated with buffered ruminal fluid. Journal of Animal Science 71, 1041–1046.Google Scholar
Beuvink, J. M. W., Spoelstra, S. F. & Hogendorp, R. J. (1992). An automated method for measuring time-course of gas production of feedstuffs incubated with buffered rumen fluid. Netherlands Journal of Animal Science 40, 401–407.Google Scholar
Blümmel, M. & Becker, K. (1997). The degradability characteristics of 54 roughages and roughage NDF as described by in vitro gas production and their relationship to voluntary feed intake. British Joumul of Nutrition 77, 757–768.Google Scholar
Blümmel, M. & Ørskov, E. R. (1993). Comparison of in vitro gas production and nylon bag degradability of roughages in prediction of feed intake in cattle. Animal Feed Science and Technology 40, 109–119.Google Scholar
Blümmel, M., Steingaβ, H. & Becker, K. (1994). The partitioning of in vitro fermentation products and its bearing for voluntary feed intake. Proceedings of the Society of Nutrition Physiology 3, 123 Abstr.Google Scholar
Blümmel, M.Steingass, H., Becker, K. & Koppenhagen, M. (1993). Produktion von SCFA, CO2, CH4, und Mikroben bei der in vitro Fermentation im Hohenheimer Futtenverttest (Production of SCFA, CO2, CH4 and microbial cells in vitro). Proceedings of the Society of Nutrition Physiology 1, 9 Abstr.Google Scholar
Crawford, R. J., Hoover, W. H. & Junkins, L. L. (1980). Effects of solids and liquids flows on fermentation in continuous culture. 2. Nitrogen partition and efficiencies of microbial synthesis. Journal of Animal Science 51, 986–993.Google Scholar
Czerkawski, J. W. & Breckenridge, G. (1970). Small apparatus for studying rumen fermentation in vitro. Laboratory Practice 19, 717–728.Google ScholarPubMed
Dahlberg, E. M., Stern, M. D. & Ehle, F. R. (1988). Effects of forage source on nuninal microbial nitrogen metabolism and carbohydrate digestion in continuous culture. Journal of Animal Science 66, 2071–2083.CrossRefGoogle ScholarPubMed
France, M., Dhanoa, M. S., Theodorou, M. K., Lister, S. J., Davies, D. R. & Isacs, D. (1993). A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163, 99–111.CrossRefGoogle Scholar
(1988). SAS/STAT Version 6.1 Cary, NC: SAS Institute Inc.Google Scholar
Stouthamer, A. H. (1973). A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie van Leeuwenhoek 39, 545–565.Google Scholar
Tempest, D. W. & Neijssel, O. M. (1984). The status of YATP and maintenance energy as biologically interpretable phenomena. Annual Review of Microbiology 38, 459–486.CrossRefGoogle ScholarPubMed
Trei, J., Hale, W. H. & Theurer, B. (1970). Effect of grain processing on in vitro gas production. Journal of Animal Science 30, 825–831.Google Scholar
Van Soest, P. J. (1994). The Nutritional Ecology ofthe Ruminant. 2nd ed. Ithaca, NY: Comell University Press.Google Scholar
Van Soest, P. & Robertson, J. B. (1985). A Laboratory Manual for Animal Science 612. Ithaca, Ny: Cornell University Press.Google Scholar
Varvikko, T. & Lindberg, J. E. (1985). Estimation of microbial nitrogen in nylon bag residues by feed 15N dilution. British Journal of Nutrition 54, 473–481.Google Scholar
Windschitl, P. M. & Stem, M. D. (1988 a). Effect of urea supplementation of diets containing lignosulfate treated soybean meal on bacterial fermentation of ruminal contents. Journal of Animal Science 66, 2948–2958.CrossRefGoogle Scholar
Windschitl, P. M. & Stem, M. D. (1988 b). Influence of methionine derivates on effluent flow of methionine from continuous culture of ruminal bacteria. Journal of Animal Science 66, 2937–2947.CrossRefGoogle ScholarPubMed
Wolin, M. J. (1960). A theoretical rumen fermentation balance. Journal of Dairy Science 43, 1452–1459.Google Scholar