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Relationship between dietary Na+ intake, aldosterone and colonic amiloride-sensitive Na+ transport

Published online by Cambridge University Press:  09 March 2007

J. Pácha  and
I. Pohlová
J. Pácha
Affiliation:
Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
I. Pohlová
Affiliation:
Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
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Abstract

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The effect of changes in dietary Na+ intake on plasma aldosterone levels and electrogenic amiloride-sensitive Na+ transport (Iamil) was studied in the rat distal colon. Five groups of rats were fed on diets containing different amounts of Na+. Estimated Na+ intake ranged from about 400–80000 ?equiv Na+ /kg body weight (BW) per d. Both variables investigated, Iamil and plasma aldosterone, depended non-linearly on Na+ intake. Reduction of the daily Na+ intake increased plasma aldosterone levels and if these levels reached the value 200 pg/ml or more then Iamil was induced. The corresponding Na+ intake was 1300 ?equiv Na+/kg BW per d. Iamil was not observed at lower aldosterone levels and higher Na+ intakes. Aldosterone infusion for 7 d produced similar changes in Iamil compared with dietary Na+-depleted animals and made the estimation of maximum transport capacity of Iamil possible. We conclude that Iamil operates only if Na+ intake decreases below minimal Na+ requirement in growing rats and that the maximum transport capacity of this pathway is reached only after very severe Na+ deprivation.

Keywords

SodiumAldosteroneColon
Type
Intake and transport of sodium
Information
British Journal of Nutrition , Volume 73 , Issue 4 , April 1995 , pp. 633 - 640
Copyright
Copyright © The Nutrition Society 1995

References

Aviv, A., Kobayahi, T., Higashino, H., Bauman, J. W. & Yu, S. S. (1982). Chronic sodium deficit in the immature rat: its effect on adaptation to sodium excess. American Journal of Physiology 242, E241–E247.Google ScholarPubMed
Benos, D. J. (1982). Amiloride: a molecular probe of sodium transport in tissues and cells. American Journal of Physiology 242, C131–C145.Google Scholar
Binder, H. J., Sandle, G. I. & Rajendran, V. M. (1991). Colonic fluid and electrolyte transport in health and disease. In The Large Intestine: Physiology, Pathophysiology and Disease, pp. 141–168 [Phillips, S.F., Pemberton, J. H. and Shorter, R. G., editors]. New York: Raven Press.Google Scholar
Cangiano, J. L., Sargent, C. R., Stitzer, S. O. & Maldonado, M. M. (1984). Renal Na+-K+-ATPase in weanling and adult spontaneously hypertensive rats. Proceedings of the Society for Experimental Biology and Medicine 177, 240–246.Google Scholar
Dixon, W. J. (1988). BMDP Statistical Software Manual. Berkeley: University of California Press.Google Scholar
Douglas, J., Hansen, J. & Catt, K. J. (1978). Relationships between plasma renin activity and plasma aldosterone in the rat after dietary electrolyte changes. Endocrinology 103, 60–65.CrossRefGoogle ScholarPubMed
Edmonds, C. J. (1981). Amiloride sensitivity of the transepithelial electrical potential and of sodium and potassium transport in rat distal colon in vivo. Journal of Physiology 313, 547–559.CrossRefGoogle ScholarPubMed
Edmonds, C. J. & Mackenzie, J. (1984). Amiloride sensitive and insensitive sodium pathways and the cellular sodium transport pool of colonic epithelium in rats. Journal of Physiology 346, 61–71.CrossRefGoogle ScholarPubMed
Foster, E. S., Zimmerman, T. W., Hayslett, J. P. & Binder, H. J. (1983). Corticosteroid alteration of active electrolyte transport in rat distal colon. American Journal of Physiology 245, G668–G675.Google ScholarPubMed
Halevy, J., Budinger, M. E., Hayslett, J. P. & Binder, H. J. (1986). Role of aldosterone in the regulation of sodium and chloride transport in the distal colon of sodium-depleted rats. Gastroenterology 91, 1227–1233.Google Scholar
Horster, M. & Lückhoff, A. (1983). Aldosterone on sodium transport of rat distal colon in long-term adrenalectomy during acute and chronic substitution. Journal of Physiology 340, 503–511.CrossRefGoogle Scholar
Lübcke, R. & Barbezat, G. O. (1988). Intestinal ion transport in rats with spontaneous arterial hypertension. Clinical Science 75, 127–133.CrossRefGoogle ScholarPubMed
Lundin, S., Herlitz, H., Norlander, M. H., Ricksten, S. E., Göthberg, G. & Berglund, G. (1982). Sodium balance during development of hypertension in the spontaneously hypertensive rat (SHR). Acta Physiologica Scandinavica 115, 317–323.CrossRefGoogle ScholarPubMed
Nishi, A., Bertorello, A. M. & Aperia, A. (1992). High salt diet down-regulates proximal tubule Na+, K+-ATPase activity in Dahl salt-resistant but not in Dahl salt-sensitive rats. evidence of defective dopamine regulation. Acta Physiologica Scandinavica 144, 263–267.CrossRefGoogle Scholar
Pácha, J., Frindt, G., Antonian, L., Silver, R. B. & Palmer, L. G. (1993). Regulation of Na channels of the rat cortical collecting tubule by aldosterone. Journal of General Physiology 102, 25–42.CrossRefGoogle ScholarPubMed
Pácha, J., Popp, M. & Capek, K. (1987). Amiloride-sensitive sodium transport of the rat distal colon during early postnatal development. Pflügers Archiv 409, 194–199.CrossRefGoogle ScholarPubMed
Pácha, J., Popp, M. & Capek, K. (1988). Corticosteroid regulation of Na+ and K+ transport in the rat distal colon during early postnatal development. Journal of Developmental Physiology 10, 531–540.Google Scholar
Perrone, R. D. & Jenks, S. L. (1984). Suppression of coupled Na-Cl absorption by aldosterone and dexamethasone in rat distal colonin vitro. American Journal of Physiology 246, F785–F793.Google Scholar
Rossier, B. C. & Palmer, L. G. (1992). Mechanisms of aldosterone action on sodium and potassium transport. In The Kidney: Physiology and Pathophysiology, pp. 1373–1409 [Seldin, D.W. and Giebisch, G., editors]. New York: Raven Press.Google Scholar
Vinson, G. P., Hinson, J. P. & Raven, P. W. (1985). The relationship between tissue preparation and function; methods for the study of control of aldosterone secretion: a review. Cell Biochemistry and Function 3, 235–253.Google Scholar
Warner, R. G. & Breuer, L. H. (1978). Nutrient requirements of the laboratory rat. In Nutrient Requirements of Laboratory Animals. Bulletin no. 10, pp. 56–93. Washington, DC: National Academy of Sciences, Committee on Animal Nutrition.Google Scholar
Will, P. C., Cortright, R. N., DeLisle, R. C., Douglas, J. G. & Hopfer, U. (1985). Regulation of amiloride- sensitive electrogenic sodium transport in the rat colon by steroid hormones. American Journal of Physiology 248, G124–G132.Google ScholarPubMed
Will, P. C., Lebowitz, J. L. & Hopfer, U. (1980). Induction of amiloride-sensitive sodium transport in the rat colon by mineralocorticoids. American Journal of Physiology 238, F261–F268.Google ScholarPubMed