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Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats

Published online by Cambridge University Press:  09 March 2007

Hiroshi Watanabe ,
Kei Sonoyama ,
Jun Watanabe ,
Natsu Yamaguchi ,
Hiroto Kikuchi ,
Taizo Nagura ,
Tsutomu Aritsuka ,
Kyoko Fukumoto  and
Takanori Kasai
Hiroshi Watanabe
Affiliation:
Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Kei Sonoyama*
Affiliation:
Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Jun Watanabe
Affiliation:
Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Natsu Yamaguchi
Affiliation:
Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Hiroto Kikuchi
Affiliation:
Research Center, Nippon Beet Sugar Mfg. Co. Ltd, Obihiro 080-0831, Japan
Taizo Nagura
Affiliation:
Research Center, Nippon Beet Sugar Mfg. Co. Ltd, Obihiro 080-0831, Japan
Tsutomu Aritsuka
Affiliation:
Research Center, Nippon Beet Sugar Mfg. Co. Ltd, Obihiro 080-0831, Japan
Kyoko Fukumoto
Affiliation:
Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
Takanori Kasai
Affiliation:
Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
*
*Corresponding author: fax +81 11 706 2496, email [email protected]
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Abstract

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Oral administration of raffinose, a naturally occurring indigestible oligosaccharide, has reportedly ameliorated atopic dermatitis in human subjects although the mechanism is unknown. The present study investigated the effect of dietary raffinose on allergen-induced airway eosinophilia in ovalbumin-sensitised Brown Norway rats as an atopic disease model. Brown Norway rats were immunised by subcutaneous injection with ovalbumin on day 0 and fed either a control diet or the diet supplemented with raffinose (50 g/kg diet). The rats were exposed to aerosolised ovalbumin on day 20, and broncho-alveolar lavage fluid was obtained on the next day. The number of eosinophils in the fluid was significantly lower in the rats fed the raffinose diet than in those fed the control diet. Dietary raffinose significantly reduced IL-4 and IL-5 mRNA levels in lung tissue and tended to lower ovalbumin-specific Ig E levels. Suppression of eosinophilia by dietary raffinose was still observed in caecectomised and neomycin-administered rats, suggesting little contribution by the colonic bacteria to the effect of raffinose. Intraperitoneal administration of raffinose also suppressed eosinophilia. Significant concentrations of raffinose were detected in portal venous and abdominal arterial plasma after the intragastric administration of raffinose. Overall, the findings suggest that dietary raffinose ameliorates allergic airway eosinophilia at least partly via post-absorptive mechanisms in Brown Norway rats.

Keywords

RaffinoseEosinophiliaAllergyOvalbuminBroncho-alveolar lavage fluid
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 2 , August 2004 , pp. 247 - 255
Copyright
Copyright © The Nutrition Society 2004

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