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Rats treated with oleoyl-oestrone maintain glucidic homeostasis: comparisons with a pair-fed model

Published online by Cambridge University Press:  08 March 2007

Anna Salas ,
Montserrat Esteve ,
M. Mar Grasa  and
Xavier Remesar
Anna Salas
Affiliation:
Department de Nutrició i Bromatologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, 08028 Barcelona, Spain
Montserrat Esteve
Affiliation:
Department de Nutrició i Bromatologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, 08028 Barcelona, Spain
M. Mar Grasa
Affiliation:
Department de Nutrició i Bromatologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, 08028 Barcelona, Spain
Xavier Remesar*
Affiliation:
Department de Nutrició i Bromatologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, 08028 Barcelona, Spain
*
*Corresponding author: Prof. Dr Xavier Remesar, fax +34 93 403 70 64, email [email protected]
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Abstract

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To determine whether or not the weight (and fat) loss induced by oleoyl-oestrone treatment results only as a consequence of decreased food intake, we compared treated animals with a pair-fed model. To this end, Wistar female rats received daily oral gavages of 10μmol/kg per d oleoyl-oestrone in sunflower oil, or vehicle alone for 10 or 20d. A second group of rats received the gavage of sunflower oil and the same amount of food ingested as the oleoyl-oestrone-treated animals (pair-fed group). Rats treated with oleoyl-oestrone maintained glucidic metabolism homeostasis despite a marked decrease in adipose tissue weight (P<0·001). Pair-fed rats exhibited a different pattern, comparable to short-term starvation, with greatly decreased glycogen stores (P<0·0001). The most significant effects were detected in the 10d period groups. Oleoyl-oestrone affected the activity of the ponderostat system not only by decreasing appetite but also by modifying energy partition: treated animals maintained their glucose and energy homeostasis despite decreased food intake and the massive depletion of lipid stores.

Keywords

Oleoyl-oestroneBody weightPair-fed
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 738 - 745
Copyright
Copyright © The Nutrition Society 2005

References

Adán, C, Cabot, C, Vilà, R, Grasa, MM, Masanés, RM, Esteve, M, Estruch, J, Fernández-López, JA, Remesar, X & Alemany, M (1999) Oleoyl-oestrone treatment affects the ponderostat setting differently in lean and obese Zucker rats. Int J Obes 23, 366–373.CrossRefGoogle Scholar
Ardévol, A, Virgili, J, Sanchis, D, Adán, C, Fernández-Real, JM, Fernández-López, JA, Remesar, X & Alemany, M (1997) A method for the measurement of plasma oestrone fatty ester levels. Anal Biochem 249, 247–250.CrossRefGoogle ScholarPubMed
Baranowska, B, Chmielowska, M, Wolinska-Witort, E, Roguski, K & Wasilewska-Dziubinska, E (2001) The relationship between neuropeptides and hormones in starvation. Neuro Endocrinol Let 22, 249–355.Google ScholarPubMed
Belda, X, Ons, S, Carrasco, J & Armario, A (2005) The effects of chronic food restriction on hypothalamic-pituitary-adrenal activity depend on morning versus evening availability of food. Pharmacol Biochem Behav 81, 41–46.CrossRefGoogle ScholarPubMed
Blay, M, Peinado-Onsurbe, J, Grasa, MM, Díaz-Silva, M, Fernández-López, JA, Remesar, X & Alemany, M (2002) Effect of oral oleoyl-oestrone treatment on plasma lipoproteins and tissue lipase activities of Zucker lean and obese female rats. Int J Obes 26, 618–626.CrossRefGoogle Scholar
Cabot, C, Grasa, MM, Masanés, RM, de Matteis, R, Cinti, S, Fernández-López, JA, Remesar, X & Alemany, M (2001) Oleoyl-oestrone does not have direct estrogenic effects on rats. Life Sci 69, 749–761.CrossRefGoogle Scholar
Cabot, C, Masanés, R, Bulló, M, García-Lorda, P, Fernández-López, JA, Salas-Salvadó, J & Alemany, M (2000) Plasma acyl-oestrone levels are altered in obese women. Endocr Res 26, 465–476.CrossRefGoogle Scholar
Comizio, R, Pietrobelli, A, Tan, YX, Wang, Z, Withers, RT, Heymsfield, SB & Boozer, CN (1998) Total lipid and triglyceride response to energy deficit: relevance to body composition models. Am J Physiol 274, E860–E866.Google ScholarPubMed
Esteve, M, Savall, P, Virgili, J, Fernández-López, JA, Remesar, X & Alemany, M (2001) Modulation by leptin, insulin and corticosterone of oleoyl-oestrone synthesis in cultured 3T3L1 cells. Biosci Rep 21, 755–763.CrossRefGoogle Scholar
Fernández-Real, JM, Sanchis, D, Ricart, W, Casamitjana, R, Balada, F, Remesar, X & Alemany, M (1999) Plasma ooestrone-fatty acid ester levels are correlated with body fat mass in humans. Clin Endocrinol 50, 253–260.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497–509.CrossRefGoogle ScholarPubMed
Grasa, MM, Cabot, C, Esteve, M, Yubero, P, Masanés, RM, Blay, MT, Vilà, R, López-Martí, J, Fernández-López, JA, Remesar, X & Alemany, M (2001) Daily oral oleoyl-oestrone gavage induces a dose-dependent loss of fat in Wistar rats. Obes Res 9, 202–209.CrossRefGoogle Scholar
Her, PA, Hammond, RP & Homolsky, MW (1973) Sodium pump activity during norepinephrine-stimulated respiration in brown adipocytes. Am J Physiol 224, 1300–1304.Google Scholar
Koopman, SJ, Sips, HC, Bosman, J, Radder, JK & Krans, HM (1989) Antilipolytic action of insulin in adipocytes from starved and diabetic rats during adenosine-controlled incubations. Endocrinology 125, 3044–3050.CrossRefGoogle Scholar
Palou, A, Remesar, X, Arola, L, Herrera, E & Alemany, M (1981) Metabolic effects of short-term food deprivation in the rat. Hormone Metab Res 13, 326–330.CrossRefGoogle ScholarPubMed
Porter, MH, Fine, JB, Cutchings, AG, Bai, Y & DiGirolamo, M (2004) Sexual dimorphism in the response of adipose mass and cellularity to graded caloric restriction. Obesity Res 12, 131–140.CrossRefGoogle ScholarPubMed
Rabinovitch, A, Grill, V, Renold, AE & Cerasi, E (1976) Insulin release and cyclic AMP accumulation in response to glucose in pancreatic islets of fed and starved rats. J Clin Invest 58, 1209–1216.CrossRefGoogle ScholarPubMed
Remesar, X, Fernández-López, JA, Blay, MT, Savall, P, Salas, A, Díaz-Silva, M, Esteve, M, Grasa, MM & Alemany, M (2002) Effect of oleoyl-oestrone on adipose tissue composition in male rats. Int J Obes 26, 1092–1102.CrossRefGoogle Scholar
Sanchis, D, Adán, C, Ardévol, A, Grasa, MM, Cabot, C, Balada, F, Vilá, R, Estruch, J, Puerta, M, Fernández-López, JA, Remesar, X & Alemany, M (1997a) Short-term treatment with oleoyl-oestrone in liposomes (Merlin-2) strongly reduces the expression of the ob gene in young rats. Biochem J 326, 357–360.CrossRefGoogle ScholarPubMed
Sanchis, D, Balada, F, Grasa, MM, Virgili, J, Peinado, J, Monserrat, C, Fernández-López, JA, Remesar, X & Alemany, M (1996) Oleoyl-oestrone induces the loss of body fat in rats. Int J Obes 20, 588–594.Google Scholar
Sanchis, D, Balada, F, Picó, C, Grasa, MM, Virgili, J, Farrerons, C, Palou, A, Fernández-López, JA, Remesar, X & Alemany, M (1997b) Rats receiving the slimming agent Oleoyl-oestrone in liposomes (Merlin-2) decrease food intake but maintain thermogenesis. Archiv Physiol Biochem 105, 663–672.CrossRefGoogle Scholar
Serafini, MT & Alemany, M (1987) A micro method for the enzymatic estimation of the degree of glycogen ramification. J Biochem Biophys Meth 15, 33–39.CrossRefGoogle Scholar
Troyer, D & Fernandes, G (1996) Nutrition and apoptosis. Nutr Res 16, 1959–1987.CrossRefGoogle Scholar
Zhang, Y, Matheny, M, Zolokutukhin, S, Tumer, N & Scarpace, PJ (2002) Regulation of adiponectin and leptin expression in white and brown adipose tissues: influence of beta-3-adrenergic agonists, retinoic acid, leptin and fasting. Biochem Biophys Acta 1584, 115–122.Google Scholar