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The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides – a human volunteer study

Published online by Cambridge University Press:  09 March 2007

K. M. Tuohy ,
S. Kolida ,
A. M. Lustenberger  and
G. R. Gibson
K. M. Tuohy*
Affiliation:
Food Microbial Sciences Unit, School of Food Biosciences, The University of Reading, Reading RG6 6AP, UK
S. Kolida
Affiliation:
Food Microbial Sciences Unit, School of Food Biosciences, The University of Reading, Reading RG6 6AP, UK
A. M. Lustenberger
Affiliation:
Novartis Nutrition Research AG, 3176 Neuenegg, Switzerland
G. R. Gibson
Affiliation:
Food Microbial Sciences Unit, School of Food Biosciences, The University of Reading, Reading RG6 6AP, UK
*
Corresponding author: Dr Kieran Tuohy, fax +44 118 9357222, email [email protected]
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Abstract

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Prebiotics are non-digestible food ingredients that target selected groups of the human colonic microflora, thus having the ability to alter the composition towards a more ‘beneficial’ community, i.e. selectively increasing populations of bifidobacteria and/or lactobacilli. In the present study the prebiotic potential of partially hydrolysed guar gum (PHGG) and fructo-oligosaccharides (FOS) in a biscuit was assessed in human volunteers. Fluorescent in situ hybridization using oligonucleotide probes targeting Bacteroides spp., Bifidobacterium spp., Clostridium spp. and Lactobacillus–Enterococcus spp. were used for the bacteriology and total bacteria were enumerated using the fluorescent stain 4′,6-diamidino-2-phenylindole. Thirty-one volunteers consumed daily either three experimental biscuits (providing a total (g/d) of 6·6 FOS and 3·4 PHGG) or three placebo biscuits for two 21-d crossover periods. Bifidobacteria significantly increased in number on ingestion of the experimental biscuits compared with pre-treatment and placebo population levels. Bifidobacterial numbers returned to pretreatment levels within 7 d of the cessation of intake of experimental biscuits. A correlation was observed between the initial faecal bifidobacterial numbers and the magnitude of bifidogenesis, with volunteers who possessed low initial population levels of bifidobacteria experiencing the greatest increase in bifidogenesis. No changes were observed in the other bacterial groups monitored during the trial. Thus, the prebiotic nature of FOS and PHGG was maintained in a final food product as evidenced from the selective increase in bifidobacterial numbers.

Keywords

Gut microfloraPrebioticGene probes
Type
Research Article
Information
British Journal of Nutrition , Volume 86 , Issue 3 , September 2001 , pp. 341 - 348
Copyright
Copyright © The Nutrition Society 2001

References

Alam, NH, Meier, R, Sarker, SA, Bardhan, PK, Fuchs, GJ, Schneider, H, Mahalanabis, D & Gyr, K (1997) Efficacy of a soluble fibre supplemented oral rehydration solution (ORS) in the treatment of acute diarrhoea in children. Gastroenterology 112,.Google Scholar
Amann, RI, Ludwig, W & Schleifer, K-H (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiology Reviews 59, 143–169.CrossRefGoogle ScholarPubMed
Ames, JM, Wynne, A, Hofmann, A, Plos, S & Gibson, GR (1999) The effect of a model melanoidin mixture in faecal bacterial populations in vitro. British Journal of Nutrition 82, 489–495.CrossRefGoogle Scholar
Andersson, HB, Ellegård, LH & Bosaeus, IG (1999) Nondigestibility characteristics of inulin and oligofructose in humans. Journal of Nutrition 129, 1428S–1429S.CrossRefGoogle ScholarPubMed
Bernet, M-F, Brassart, D, Neeser, J-R & Servin, AL (1993) Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen–cell interactions. Applied and Environmental Microbiology 59, 4121–4128.CrossRefGoogle ScholarPubMed
Bouhnik, Y, Kouroche, V, Achour, L, Attar, A, Salfati, J, Pochart, P, Marteau, P, Flourié, B, Bornet, F & Rambaud, J-C (1999) Short chain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans. Journal of Nutrition 129, 113–116.CrossRefGoogle ScholarPubMed
Campbell, H & Jones, I (1996) Promoting breastfeeding: a view of the current position and a proposed agenda for action in Scotland. Journal of Public Health Medicine 18, 406–414.CrossRefGoogle Scholar
Collins, MD & Gibson, GR (1999) Probiotics, prebiotics and synbiotics: approaches for the nutritional modulation of microbial ecology. American Journal of Clinical Nutrition 69, 1052s–1057s.CrossRefGoogle Scholar
Conway, PL (1995) Microbial ecology of the human large intestine. In Human Colonic Bacteria: Role in Nutrition, Physiology and Pathology pp. 1–24 [Gibson, GR and Macfarlane, GT, editors]. Boca Raton, FL: CRC Press.Google Scholar
Ducluzeau, R (1989) Role of experimental ecology in gastroenterology. In Microbial Ecology and Intestinal Infections, pp. 1–5 [Bergogne-Berezin, E, editor]. Paris: Springer.CrossRefGoogle Scholar
Franks, AH, Harmsen, HJM, Raangs, GC, Jansen, GJ, Schut, F & Welling, GW (1998) Variations of bacterial populations in human faeces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Applied and Environmental Microbiology 64, 3336–3345.CrossRefGoogle ScholarPubMed
Fuller, R & Gibson, GR (1997) Modification of the intestinal microflora using probiotics and prebiotics. Scandinavian Journal of Gastroenterology 32, 28–31.CrossRefGoogle Scholar
Gibson, GR (1998) Dietary modulation of the human gut microflora using prebiotics. British Journal of Nutrition 80, S209–S212.CrossRefGoogle ScholarPubMed
Gibson, GR & Beatty, ER, Wang, X & Cummings, JH (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975–982.CrossRefGoogle ScholarPubMed
Gibson, GR, Beaumont, A, (1996) An overview of human colonic bacteriology in health and disease. In Gut Flora and Health – Past, Present and Future. International Congress and Symposium Series 219, pp. 3–11. [Leeds, AR and Rowland, IR, editors]. London: The Royal Society of Medicine Press Ltd.Google Scholar
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microflora – introducing the concept of prebiotics. Journal of Nutrition 125, 1401–1412.CrossRefGoogle Scholar
Gibson, GR & Wang, X (1994) Regulatory effects of bifidobacteria on the growth of other colonic bacteria. Journal of Applied Bacteriology 77, 412–420.CrossRefGoogle ScholarPubMed
Gibson, GR, Williams, A, Reading, S & Collins, MD (1996) Fermentation of non-digestible oligosaccharides by human colonic bacteria. Proceedings of the Nutrition Society 55, 899–912.CrossRefGoogle ScholarPubMed
Harmsen, HJM, Elfferich, P, Schut, F & Welling, GW (1999) A 16S rRNA-targeted probe for detection of lactobacilli and enterococci in faecal samples by fluorescent in situ hybridization. Microbiol Ecology in Health Disease 11, 3–12.CrossRefGoogle Scholar
Holzapfel, WH, Haberer, P, Snel, J, Schillinger, U & Huis in't Veld, JHJ (1998) Overview of gut flora and probiotics. International Journal of Food Microbiology 41, 85–101.CrossRefGoogle ScholarPubMed
Homann, HH, Kemen, M, Fuessenich, C, Senkal, M & Zumtobel, V (1994) Reduction in diarrhoea incidence by soluble fibre in patients receiving total or supplemental enteral nutrition. Journal of Parenteral and Enteral Nutrition 18, 486–490.CrossRefGoogle ScholarPubMed
Hudson, MJ & Marsh, PD, (1995) Carbohydrate metabolism in the colon. In Human Colonic Bacteria: Role in Nutrition, Physiology and Pathology, pp. 61–73. [Gibson, GR and MacFarlane, GT, editors]. Boca Raton, FL: CRC Press.Google Scholar
Langendijk, PS, Schut, F, Jansen, GJ, Raangs, GW, Kamphuis, GR, Wilkinson, MHF & Welling, GW (1995) Quantitative fluorescent in situ hybridazation of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in faecal samples. Applied and Environmental Microbiology 61, 3069–3075.CrossRefGoogle Scholar
Levitt, MD, Gibson, GR & Christl, SU (1995) Gas metabolism in the large intestine Human Colonic Bacteria: Role in Nutrition, Physiology and Pathology, pp. 131–154 [Gibson, GR and MacFarlane, GT, editors]. Boca Raton, FL: CRC Press.Google Scholar
McCracken, VJ & Gaskins, HR (1999) Probiotics and the immune system. In Probiotics: A Critical Review pp. 85–111 [Tannock, GW, editor]. Wymondham:Norfolk: Horizon Scientific Press.Google Scholar
MacFarlane, GT & Gibson, GR, (1997) Carbohydrate fermentation, energy transduction and gas metabolism in the human large intestine. In Gastrointestinal Microbiology. vol 1, pp. 269–318 [Mackie, RI and Placebo, BH, editors]. London: Chapman and Hall.CrossRefGoogle Scholar
Manz, W, Amann, R, Ludwig, W, Vancanneyt, M & Schleifer, K-H (1996) Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology 142, 1097–1106.CrossRefGoogle ScholarPubMed
Nelson, GM & George, SE (1995) Comparison of media for selection and enumeration of mouse fecal flora populations. Journal of Microbiology 22, 293–300.Google Scholar
Okubo, T, Ishihara, N, Takahashi, H, Fujisawa, T, Kim, M, Yamamoto, T & Mitsuoka, T (1994) Effects of partially hydrolyzed guar gum on intestinal microflora and its metabolism. Bioscience Biotechnology and Biochemistry 58, 1364–1369.CrossRefGoogle Scholar
Roberfroid, MD, Van Loo, JAE & Gibson, GR (1998) The bifidogenic nature of chicory inulin and its hydrolysis products. Journal of Nutrition 128, 11–19.CrossRefGoogle ScholarPubMed
Saavedra, JM, Bauman, NA, Oung, I, Perman, JA & Yolken, RH (1994) Feeding of Bifidobacterium bifidumStreptococcus thermophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet 344, 1046–1049.CrossRefGoogle ScholarPubMed
Steer, T, Carpenter, H, Tuohy, K & Gibson, GR (2000) Perspectives on the role of the human gut microbiota in health and methods of study. Nutrition Research Reviews 13, 229–254.CrossRefGoogle Scholar
Suau, A, Bonnet, R, Sutren, M, Godon, J-J, Gibson, GR, Collins, MD & Doré, J (1999) Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Applied and Environmental Microbiology 65, 4799–4807.CrossRefGoogle ScholarPubMed
Takahashi, H, Yang, SI, Hayaski, C, Kim, M, Yamanaka, J & Yamamoto, T (1994) Influence of partially hydrolyzed guar gum on constipation in women. Journal of Nutritional Science and Vitaminology 40, 251–259.CrossRefGoogle ScholarPubMed
Tannock, GW, (editor) (1999) A fresh look at the intestinal microflora. In Probiotics: A Critical Review, pp. 5–14Wymondham, Norfolk: Horizon Scientific Press.Google Scholar
Vanderhoof, JA & Young, RJ (1998) Use of probiotics in childhood gastrointestinal disorders. Journal of Pediatric Gastroenterology and Nutrition 27, 323–332.Google ScholarPubMed
Ward, DM, Bateson, MM, Weller, R & Ruff-Roberts, AL (1992) Ribosomal RNA analysis of microorganisms as they occur in nature. Advances in Microbial Ecology 12, 219–286.CrossRefGoogle Scholar
Wilson, KH & Blitchington, RB (1996) Human colonic biota studied by ribosomal DNA sequence analysis. Applied and Environmental Microbiology 62, 2273–2278.CrossRefGoogle ScholarPubMed
Woese, CR (1987) Bacterial evolution. Microbiology Reviews 51, 221–271.CrossRefGoogle ScholarPubMed
Ziemer, CJ & Gibson, GR (1998) An overview of probiotics, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. International Dairy Journal 8, 473–479.CrossRefGoogle Scholar
Zoetendal, EG, Akkermans, ADL & De Vos, WM (1998) Temperature gradient gel electrophoresis analysis of 16S rRNA from human faecal samples reveals stable and host-specific communities of active bacteria. Applied and Environmental Microbiology 64, 3854–3859.CrossRefGoogle ScholarPubMed