Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-19T05:59:58.789Z Has data issue: false hasContentIssue false
  • English
  • Français

Postprandial lipaemia is associated with increased levels of apolipoprotein A-IV in the triacylglycerol-rich fraction and decreased levels in the denser plasma fractions

Published online by Cambridge University Press:  09 March 2007

Jean Dallongeville ,
Pascal Lebel ,
Henri-Joseph Parra ,
Gérald Luc  and
Jean-Charles Fruchart
Jean Dallongeville
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Pascal Lebel
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Henri-Joseph Parra
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Gérald Luc
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Jean-Charles Fruchart
Affiliation:
SERLIA et Unité LVSERM 325, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cédex, France
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Apolipoprotein (apo) A-IV is primarily associated with HDL or with the lipoprotein-free fraction of plasma, and in small amounts with chylomicrons and VLDL. The aim of the present study was to assess the effect of a fatty meal on the postprandial variation in plasma apo A-IV and on its distribution among lipoprotein fractions following absorption of fat. Twenty healthy male subjects participated in the study. After an overnight fast, subjects were given a fatty breakfast containing 1 g fat/kg body weight (% energy: fat 65, carbohydrate 20, protein 15). Blood samples were taken every hour during the next 10 h. Apo A-IV was measured by ELISA. Postprandial lipaemia was associated with a moderate, although significant, increase in the plasma levels of apo A-IV. Apo A- IV increased from the median baseline value of 0·15 g/1 to 0·165 g/l (median + 17 %; P < 0·01) 5 h after fat ingestion. The postprandial peak of apo A-IV occurred 1 h after the triacylglycerol peak. There were no statistically significant correlations between baseline lipids, baseline apo A-IV and postprandial changes in apo A-IV levels, or between postprandial changes in lipids and apo A-IV at any time. To assess apo A-IV distribution among lipoproteins, plasma was fractionated by fast performance liquid chromatography at baseline and 3, 6 and 10 h postprandially. There was a substantial heterogeneity in the apo A-IV distribution among lipoproteins following the fatty meal. At 3 h after fat ingestion, apo A-IV levels increased in the triacylglycerol-rich lipoprotein (TRL) fraction and decreased in the denser plasma fraction. At 6 h after the fatty meal, apo A-IV was still present in the TRL but was decreased in the HDL fractions. The findings of the present study support the concept that apo A-IV particles transfer from the denser plasma fraction to TRL during postprandial lipaemia

Keywords

Postprandial metabolismApolipoprotein A-IVLipids
Type
Human and Clinical Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 2 , February 1997 , pp. 213 - 223
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Aalto-Setälä, K., Bisgaier, C. L., Ho, A., Kielt, K. A., Traber, M. G., Kayden, H. I., Ramakrishnan, R., Walsh, A., Essenburg, A. D. & Breslow, J. L. (1994). Intestinal expression of human apolipoprotein A-IV in transgenic mice fails to influence dietary lipid absorption or feeding behaviour. Journal of Clinical Investigation 93, 1776–1786.CrossRefGoogle ScholarPubMed
Annuzi, G., Holmquist, L. & Carlson, L. A. (1989). Concentrations of apolipoproteins B, C-I, C-11, C-111, E and lipids in serum and serum lipoproteins of normal subjects during alimentary lipemia. Scandinavian Journal of Clinical and Laboratory Investigation 49, 73–81.CrossRefGoogle Scholar
Barter, P., Rajaram, O. V., Chang, L. B., Rye, K. A., Gambert, P., Lagrost, L., Ehnholm, C. & Fidge, N. H. (1988). Isolation of a high density lipoprotein conversion factor from human plasma. A possible role of apolipoprotein A-IV as its activator. Biochemical Journal 254, 179–184.CrossRefGoogle ScholarPubMed
Beisiegel, U. & Utermann, G. (1979). An apolipoprotein homolog of rat apolipoprotein A-IV in human plasma. Isolation and partial characterization. European Journal of Biochemistry 93, 601–608.CrossRefGoogle Scholar
Bisgaier, C. L., Sachdev, O. P., Megna, L. & Glickman, R. M. (1985). Distribution of apolipoprotein A-IV in human plasma. Journal of Lipid Research 26, 11–25.CrossRefGoogle ScholarPubMed
Blum, C. B. (1982). Dynamics of apolipoprotein E metabolism in humans. Journal of Lipid Research 23, 1308–1316.CrossRefGoogle ScholarPubMed
Elshourbagy, N. A., Walkers, D. W., Paik, Y., Boguski, M. S., Gordon, J. I. & Taylor, J. M. (1986). The nucleotide and derived amino acid sequence of human apolipoprotein A-IV mRNA and the close linkage of its gene to the genes of apolipoproteins A-I and C-111. Journal of Biological Chemistry 261, 1998–2002.CrossRefGoogle Scholar
Fujimoto, K., Cardelli, J. A. & Tso, P. (1992). Increased apolipoprotein A-IV in rat mesenteric lymph after lipid meal acts as a physiological signal for satiation. American Journal of Physiology 262, G1002–G1006.Google ScholarPubMed
Fujimoto, K., Machidon, H., Iwakiri, R., Yamamoto, K., Fujisaki, J., Sakate, T. & Tso, P. (1993). Effect of intravenous administration of apolipoprotein A-IV on patterns of feeding, drinking and ambulatory activity of rats. Brain Research 608, 233–237.CrossRefGoogle ScholarPubMed
Goldberg, I. J., Scheraldi, C. A., Yacoub, L. K., Saxena, U. & Bisgaier, C. L. (1990). Lipoprotein apo C-I1 activation of lipoprotein lipase: modulation by apolipoprotein A-IV. Journal of Biological Chemistry 265, 4266–4272.CrossRefGoogle Scholar
Green, P. H. R., Glickman, R. M., Riley, J. W. & Quinet, E. (1980). Human apolipoprotein A-IV. Intestinal origin and distribution in plasma. Journal of Clinical Investigation 65, 911–919.CrossRefGoogle ScholarPubMed
Green, P. H. R., Glickman, R. M., Saudeck, C. D., Blum, C. B. & Tall, A. R. (1979). Human intestinal lipoproteins. Studies in chyluric subjects. Journal of Clinical Investigation 64, 233–242.CrossRefGoogle ScholarPubMed
Havel, R. J., Kane, J. P. & Kashyap, M. L. (1973). Interchange of apolipoproteins between chylomicrons and high density lipoproteins during alimentary lipemia in man. Journal of Clinical Investigation 52, 32–38.CrossRefGoogle ScholarPubMed
Lagrost, L., Gambert, P., Boquillon, M. & Lallemant, C. (1989). Evidence for high density lipoproteins as major apolipoprotein A-IV containing fraction in normal human serum. Journal of Lipid Research 30, 1525–1534.CrossRefGoogle ScholarPubMed
Lipid Research Clinics Program (1974). Manual of Laboratory Operations. Vol. 1. Lipid and Lipoprotein Analysis Washington, DC: US Government Printing Office.Google Scholar
Ohta, T., Fidge, N. H. & Nestel, P. J. (1985). Studies on the in vivo and in vitro distribution of apolipoprotein A- IV in human plasma and lymph. Journal of Clinical Investigation 76, 1252–1260.CrossRefGoogle ScholarPubMed
Seishima, M., Norna, A., Torizawa, H. & Muto, Y. (1988). Changes of apolipoprotein levels after oral administration of fat in human subjects. Atherosclerosis 73, 39–43.CrossRefGoogle ScholarPubMed
Sherman, J. R. & Weinberg, R. B. (1988). Serum apolipoprotein A-IV and lipoprotein cholesterol in patients undergoing total parenteral nutrition. Gastroenterology 95, 394–401.CrossRefGoogle ScholarPubMed
Stein, O., Stein, Y., Lefebvre, M. & Roheim, P. S. (1986). The role of apolipoprotein A-IV in reverse cholesterol transport studied with cultured cells and liposomes derived from an ether analog of phosphatidylcholine. Biochimica et Biophysica Acta 878, 7–13.CrossRefGoogle ScholarPubMed
Steinmetz, A., Kaffarnlk, H. & Utermann, G. (1985). Activation of phosphatidylcholine-sterol acyltransferase by human apolipoprotein E isoforms. European Journal of Biochemishy 152, 747–751.CrossRefGoogle ScholarPubMed
Swift, L. L., Soulé, P. D., Gray, M. E. & LeQuire, V. S. (1984). Intestinal lipoprotein synthesis. Comparison of nascent Golgi lipoproteins from chow-fed and hypercholesterolemic rats. Journal of Lipid Research 25, 1–13.CrossRefGoogle ScholarPubMed
Traber, M. G., Kayden, H. J. & Rindler, M. J. (1987). Polarized secretion of newly synthesized lipoproteins by Caco-2 human intestinal cell line. Journal of Lipid Research 28, 1350–1363.CrossRefGoogle ScholarPubMed
Utermann, G. & Beisiegel, U. (1979). Apolipoprotein A-IV a protein occurring in human mesenteric lymph chylomicrons and free in plasma. Isolation and quantification. European Journal of Biochemishy 99, 333–343.CrossRefGoogle ScholarPubMed
Weinberg, R. B., Ibdah, J. A. & Phillips, M. C. (1992). Adsorption of apolipoprotein A-IV to phospholipid monolayers spread at the aidwater interface. Journal of Biological Chemistty 267, 8977–8983.CrossRefGoogle ScholarPubMed
Weinberg, R. B. & Spector, M. S. (1985). Human apolipoprotein A-IV. Displacement from the surface of triglyceride-rich particles by HDL2-associated C-apoproteins. Journal of Lipid Research 26, 26–37.CrossRefGoogle ScholarPubMed
Zaiou, M., Visvikis, S., Parra, H. J., Steinmetz, J., Aguillon, D., Jaid, M., Fournier, B., Boerwinckle, E. & Siest, G. (1993). Postprandial effect of an oral fat load on serum apolipoprotein A-IV concentration. In Biologie Prospective. Comptes rendus du 8e Colloque de Pont-2-Mousson, pp. 545–548 [Galteau, M. M., Siest, G. & Henny, J. editors]. Paris: John Libbey Eurotext.Google Scholar