Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-27T13:46:39.312Z Has data issue: false hasContentIssue false
  • English
  • Français

Plasma proteins in growing analbuminaemic rats fed on a diet of low-protein content

Published online by Cambridge University Press:  09 March 2007

J. A. Joles ,
E. H. J. M. Jansen ,
C. A. Laan ,
N. Willekes-Koolschijn ,
W. Kortlandt  and
H. A. Koomans
J. A. Joles
Affiliation:
Department of Nephrology and Hypertension andUniversity Hospital Utrecht, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands
E. H. J. M. Jansen
Affiliation:
National Institute of Public Health and Environmental Hygiene (RIVM), A. van Leeuwenhoeklaan 9, Bilthoven, The Netherlands
C. A. Laan
Affiliation:
National Institute of Public Health and Environmental Hygiene (RIVM), A. van Leeuwenhoeklaan 9, Bilthoven, The Netherlands
N. Willekes-Koolschijn
Affiliation:
Department of Nephrology and Hypertension andUniversity Hospital Utrecht, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands
W. Kortlandt
Affiliation:
Department of Clinical Chemistry, University Hospital Utrecht, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands
H. A. Koomans
Affiliation:
Department of Nephrology and Hypertension andUniversity Hospital Utrecht, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Analbuminaemic and Sprague-Dawley (control) rats were fed on low- (60 g/kg) protein and control (200 g protein/kg) diets ad lib. from weaning. Males and females were studied separately. Body-weight and plasma protein concentrations were determined at 10 d intervals from 25 to 75 d of age. Electrophoresis of plasma proteins was performed in samples from day 75. Extracellular fluid volume was measured at 10 d intervals from day 45 onwards. Colloid osmotic pressure was measured in plasma and interstitial fluid (wick technique) at the start and end of the trial.

2. Body-weight increased much less on the low-protein diet than on the normal diet in both strains and sexes. The growth retardation was slightly more pronounced in the male analbuminaemic rats than in the male Sprague-Dawley controls.

3. Plasma protein concentration increased during normal growth in all groups, particularly in the female analbuminaemic rats. This increase was reduced by the 60 g protein/kg diet in all groups, with the exception of the male analbuminaemic rats.

4. Differences in plasma colloid osmotic pressure were similar to those seen in plasma protein concentration. Interstitial colloid osmotic pressure was higher in the control rats than in the analbuminaemic ones. The interstitial colloid osmotic pressure increased during growth in the control but not in the analbuminaemic rats. The difference in interstitial colloid osmotic pressure between the strains was maintained during low-protein intake, but at a lower level than during normal protein intake.

5. Subtracting interstitial from plasma colloid osmotic pressure, resulted in a rather similar transcapillary oncotic gradient in the various groups at 75 d, both on the control protein diet (11–14 mmHg), and on the lowprotein diet (9–11 mmHg).

6. All protein fractions were reduced to a similar extent by the low-protein diet in the control rats, whereas in the analbuminaemic rats protein fractions produced in the liver were more severely depressed.

7. Extracellular fluid volume as a percentage of body-weight was similar in all groups, and decreased with increasing age.

8. In conclusion, the analbuminaemic rats were able to maintain the transcapillary oncotic gradient on both diets by reducing the interstitial colloid osmotic pressure. Oedema was not observed.

9. Despite the absence of albumin, the protein-malnourished analbuminaemic rat is no more susceptible to hypoproteinaemia and oedema than its normal counterpart.

Type
Protein, Growth and Development
Information
British Journal of Nutrition , Volume 61 , Issue 3 , May 1989 , pp. 485 - 494
Copyright
Copyright © The Nutrition Society 1989

References

Alleyne, G. A. O., Hay, R. W., Picou, D. I., Stanfield, J. P. & Whitehead, R. G. (1977). Protein-Energy Malnutrition pp. 1–7. London: Edward Arnold.Google Scholar
Anon. (1970). Classification of infantile malnutrition. Lancet ii, 302–303.Google Scholar
Aukland, K. & Nicolaysen, G. (1981). Interstitial fluid volume: local regulatory mechanisms. Physiological Reviews 61, 556–643.CrossRefGoogle ScholarPubMed
Barratt, T. M. & Walser, M. (1969). Extracellular fluid in individual tissues and in whole animals: the distribution of radiosulphate and radiobromide. Journal of Clinical Investigation 48, 56–66.Google Scholar
Cartlidge, P. H. T. & Rutter, N. (1986). Serum albumin concentrations and oedema in the newborn. Archives of Disease in Childhood 61, 656–660.CrossRefGoogle ScholarPubMed
Coward, W. A. (1975). Serum colloidal osmotic pressure in the development of kwashiorkor and in recovery: its relationship to albumin and globulin concentrations and oedema. British Journal of Nutrition 34, 459–467.Google Scholar
Coward, W. A. & Lunn, P. G. (1981). The biochemistry and physiology of kwashiorkor and marasmus. British Medical Bulletin 37, 19–24.CrossRefGoogle ScholarPubMed
Coward, W. A. & Sawyer, M. B. (1977). Whole-body albumin mass and distribution in rats fed on low-protein diets. British Journal of Nutrition 37, 127–134.Google Scholar
Daufeldt, J. A. & Harrison, H. H. (1984). Quality control and technical outcome of iso-dalt two-dimensional electrophoresis in a clinical laboratory setting. Clinical Chemistry 20, 1972–1980.CrossRefGoogle Scholar
Edozien, J. C. (1968). Experimental kwashiorkor and marasmus. Nature 220, 917–919.Google Scholar
Emori, T., Takahashi, M., Sugiyama, K., Shumiya, S. & Nagase, S. (1983). Age-related changes in plasma proteins of analbuminemic rats. Experimental Animals 32, 123–132.CrossRefGoogle ScholarPubMed
Esumi, H., Sato, S., Ouki, M., Sugimura, T. & Nagase, S. (1979). Turnover of serum proteins in rats with analbuminemia. Biochemical and Biophysical Research Communications 87, 1191–1199.CrossRefGoogle ScholarPubMed
Esumi, H., Takahashi, Y., Seki, M., Sato, S., Nagase, S. & Sugimura, T. (1982). Perinatal changes of α-fetoprotein concentration in the serum and its synthesis in the liver of analbuminemic rats. Cancer Research 42, 306–308.Google ScholarPubMed
Fadnes, H. O. (1975). Protein concentration and hydrostatic pressure in subcutaneous tissue of rats in hypoproteinemia. Scandinavian Journal of Clinical and Laboratory Investigation 35, 441–446.Google Scholar
Fiorotto, M. & Coward, W. A. (1979). Pathogenesis of oedema in protein-energy malnutrition: the significance of plasma colloid osmotic pressure. British Journal of Nutrition 42, 21–31.CrossRefGoogle ScholarPubMed
Hanna, F. M. (1983). Changes in body composition of normal infants in relation to diet. Annals of the New York Academy of Sciences 110, 840–848.CrossRefGoogle Scholar
Johnsen, H. M. (1974). Measurement of colloid osmotic pressure of interstitial fluid. Acta Physiologica Scandinavica 91, 142–144.CrossRefGoogle ScholarPubMed
Joles, J. A., Koomans, H. A., Kortlandt, W., Boer, P. & Dorhout Mees, E. J. (1988). Hypoproteinemia and recovery from edema in dogs. American Journal of Physiology 254, F887–F894.Google Scholar
Kirsch, R., Frith, L., Black, E. & Hoffenberg, R. (1968). Regulation of albumin synthesis and catabolism by alteration of dietary protein. Nature 217, 578–579.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–687.CrossRefGoogle ScholarPubMed
Lanzillo, J. J., Stevens, J. & Fanburg, B. L. (1980). A comparison of commonly used discontinuous and continuous buffer systems for electrophoresis in sodium dodecyl sulphate containing polyacrylamide gels. Electrophoresis 1, 180–186.CrossRefGoogle Scholar
Lewandowski, A. E., Liao, W. S. L., Stinton-Fisher, C. A., Kent, J. D. & Jefferson, L. S. (1988). Effects of experimentally induced nephrosis on protein synthesis in the rat liver. American Journal of Physiology 254, C634–C642.CrossRefGoogle ScholarPubMed
Lunn, P. G. & Austin, S. (1983). Dietary manipulation of plasma albumin concentration. Journal of Nutrition 113, 1791–1802.CrossRefGoogle ScholarPubMed
Nagase, S., Shimamune, K. & Shumiya, S. (1979). Albumin-deficient rat mutant. Science 205, 590–591.CrossRefGoogle ScholarPubMed
Noddeland, H. S., Riisness, S. M. & Fadnes, H. O. (1982). Interstitial fluid colloid osmotic and hydrostatic pressures in subcutaneous tissue of patients with the nephrotic syndrome. Scandinavian Journal of Clinical and Laboratory Investigation 42, 139–146.CrossRefGoogle ScholarPubMed
Renkin, E. M. (1986). Some consequences of capillary permeability to macromolecules: Starling's hypothesis reconsidered. American Journal of Physiology 250, H706–H710.Google ScholarPubMed
Sugiyama, K., Emori, T. & Nagase, S. (1982). Synthesis and secretion of plasma protein by isolated hepatocytes of analbuminemic rats. Journal of Biochemistry 92, 775–779.CrossRefGoogle ScholarPubMed
Sugiyama, K., Izumi, S., Tomino, S. & Nagase, S. (1987). A high level of transferrin mRNA in the liver of analbuminemic rats. Journal of Biochemistry 102, 967–970.Google Scholar
Tripathi, A. M., Agrawal, K. K. & Agarwal, K. N. (1983). Oedema fluid composition in childhood disorders. Acta Paediatrica Scandinavica 72, 741–745.CrossRefGoogle ScholarPubMed