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Plasma 65Zn Kinetics in the rat

Published online by Cambridge University Press:  09 March 2007

N. M. Lowe ,
I. Bremner  and
M. J. Jackson
N. M. Lowe
Affiliation:
Department of Medicine, University of Liverpool, PO Box 147, Liverpool L69 3BX
I. Bremner
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
M. J. Jackson
Affiliation:
Department of Medicine, University of Liverpool, PO Box 147, Liverpool L69 3BX
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Abstract

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The amount of 65Zn in the plasma of rats after intravenous injection was found to decline following closely two-compartment kinetics over a period of 90 min. Comparative analysis of the amount of 65Zn present in the two kinetic pools at various time-intervals post-injection with the actual physiological location of the 65Zn, revealed that the initial pool (Qa) is primarily the blood plasma, while the second pool (Qb) is primarily within the liver. The plasma Zn concentration and Qa were both found to fall reproducibly during Zn depletion, whereas Qa and Qb increased following injection of Escherichia coli endotoxin in contrast to the decline in plasma Zn concentration. Further investigation of the nature of Qb indicates that it represents in part a metabolic pool within the liver which varies substantially in response to Zn status in a manner similar to metallothionein.

Keywords

ZincRat
Type
Trace Element Metabolism
Information
British Journal of Nutrition , Volume 65 , Issue 3 , May 1991 , pp. 445 - 455
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Chesters, J. K. & Will, M. (1981 a). Measurement of flux through plasma in normal and endotoxin-stressed pigs and the effects of Zn supplementation during stress. British Journal of Nutrition 46, 119–130.CrossRefGoogle ScholarPubMed
Chesters, J. K. & Will, M. (1981 b). Zinc transport proteins in plasma. British Journal of Nutrition 46, 111–118.CrossRefGoogle ScholarPubMed
Constable, B. J. (1963). Changes in blood volume and blood picture during the life of the rat and guinea pig from birth to maturity. Journal of Physiology 167, 229–266.CrossRefGoogle ScholarPubMed
Cousins, R. J. & Leinart, A. S. (1988). Tissue-specific regulation of zinc metabolism and metallothionein genes by interleukin I. FASEB Journal 2, 2884–2890.CrossRefGoogle Scholar
Dunn, M. A. & Cousins, R. J. (1989). Kinetics of zinc metabolism in the rat: effect of dibutyryl cAMP. American Journal of Physiology 256, E420–E430.Google Scholar
Giroux, E. L. (1975). Determination of zinc distribution between albumin and α2-macroglobulin in human serum. Biochemical Medicine 12, 256–266.CrossRefGoogle Scholar
Hallbook, R. & Hedelin, H. (1977). Zinc metabolism and surgical trauma. British Journal of Surgery 64, 271–273.CrossRefGoogle ScholarPubMed
Jackson, M. J., Guigliano, R., Guigliano, L. G., Oliveira, E. F., Shrimpton, R. & Swainbank, I. G. (1988). Stable isotope metabolic studies of zinc nutrition in slum-dwelling lactating women in the Amazon valley. British Journal of Nutrition 59, 193–203.CrossRefGoogle ScholarPubMed
Jackson, M. J., Jones, D. A. & Edwards, R. H. T. (1982). Tissue zinc levels as an index of body zinc status. Clinical Physiology 2, 333–343.CrossRefGoogle ScholarPubMed
Jones, R. B., Keeling, P. W. N., Hilton, P. J. & Thompson, R. P. H. (1981). The relationship between leucocyte and muscle zinc in health and disease. Clinical Science 60, 237–239.CrossRefGoogle ScholarPubMed
Keeling, P. W. N. & Thompson, R. P. H. (1983). Tissue zinc status in patients with chronic liver disease. Progress in Clinical and Biological Research 129, 235–254.Google Scholar
Krezoski, K., Villalobos, J., Shaw, C. F. & Petering, D. H. (1988). Kinetic ability of zinc bound to metallothionein in Ehrlick cells. Biochemical Journal 255, 483–491.Google Scholar
Lyon, T. D., Smith, H. & Smith, L. B. (1979). Zinc deficiency in the West of Scotland? A dietary intake study. British Journal of Nutrition 42, 413–416.Google Scholar
Mehra, R. K. & Bremner, I. (1983). Development of a radioimmunoassay for rat liver metallothionein-I and its application to the analysis of rat plasma and kidneys. Biochemical Journal 213, 459–465.CrossRefGoogle Scholar
Prasad, A. S., Halsted, J. A. & Nadami, M. (1961). Syndrome of iron deficiency anaemia hepatosplenomegaly, hypogonadism, dwarfism and geophagia. American Journal of Medicine 31, 532–546.CrossRefGoogle ScholarPubMed
Sato, M., Mehra, R. K. & Bremner, I. (1984). Measurement of plasma metallothionein-I in the assessment of zinc status in zinc-deficient and stressed rats. Journal of Nutrition 114, 1683–1689.Google Scholar
Shipley, R. A. & Clark, R. E. (1972). Tracer Methods for ‘in vivo’ Kinetics. New York: Academic Press.Google Scholar
Solomons, N. W. (1979). On the assessment of zinc and copper status in man. American Journal of Clinical Nutrition 32, 856–871.CrossRefGoogle Scholar
Steinberg, S. E. (1985). Zinc deficiency in Crohn's disease. Comparative Therapeutics 11, 34–38.Google Scholar
Wastney, M. E., Aamodt, R. L., Rumble, W. F. & Henkin, R. I. (1986). Kinetic analysis of Zn metabolism and its regulation in normal humans. American Journal of Physiology 251, R398–R408.Google ScholarPubMed