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Oral immunoadjuvant activity of Lactobacillus casei subsp. casei in dextran-fed layer chickens

Published online by Cambridge University Press:  08 March 2007

Tomohiko Ogawa ,
Yasuyuki Asai ,
Hiromi Sakamoto  and
Kenji Yasuda
Tomohiko Ogawa*
Affiliation:
Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan
Yasuyuki Asai
Affiliation:
Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan
Hiromi Sakamoto
Affiliation:
Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan
Kenji Yasuda
Affiliation:
Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan
*
*corresponding author: Dr Tomohiko Ogawa, fax +81 58 329 1421, email [email protected]
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Abstract

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We recently reported that synbiotic Lactobacillus casei subsp. casei together with specific substrate dextran elicited an enhancement in humoral immune response against bovine serum albumin (BSA) as a model antigen in BALB/c mice. The present study was designed to evaluate the oral immunoadjuvant effects of the synbiotic in layer chickens. Using a PCR assay, L. casei subsp. casei was detected specifically in the intestinal chyme of chickens (10d of age, Julia strain) fed ad libitum on a diet supplemented with 75mg dextran/kg (dextran-supplemented diet, DSD) and administered orally with 107 colony-forming units (CFU) L. casei subsp. casei in 0·1ml PBS with the aid of an intubation needle at 1, 2 and 3d of age. Furthermore, oral administration of 107 CFU L. casei subsp. casei at 1–3d of age significantly enhanced the production of anti-BSA antibody in DSD-fed chickens (60d of age) administered orally with 1mg BSA at 32 and 33d of age and subcutaneously with 5μg BSA at 33d of age. In addition, among bacterial numbers tested, 106 CFU L. casei subsp. casei together with dextran induced an effective increase in humoral immune response to mixed inactivated vaccines against Newcastle disease and avian infectious bronchitis, and the treatment may be advantageous in protecting against these infectious diseases in chickens in actual application. These results suggest that dietary supplementation of L. casei subsp. casei with dextran leads to immunomodulation of humoral immune responses.

Keywords

Lactobacillus caseiDextranSynbioticChicken
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 430 - 434
Copyright
Copyright © The Nutrition Society 2006

References

Agriculture, Forestry and Fisheries Research Council Secretariat Japanese Feeding Standard for Poultry. Tokyo, Japan: Agriculture, Forestry and Fisheries Research Council. 1999Google Scholar
Ahrne, S, Nobaek, S, Jeppsson, B, Adlerberth, IWold, AE & Molin, GThe normal Lactobacillus flora of healthy human rectal and oral mucosa. J Appl Microbiol 1998 85, 88–94.Google Scholar
Alexander, DJNewcastle disease and other avian Paramyxoviridae infection. In Disease of PoultNery. 10th ed., pp.541–570 [Calnek, BWBarnes, JHBeard, CWMcDougald, LRSaif, YMeditor].Ames, IA: Iowa State University Press. 1997Google Scholar
Bailey, JS, Blankenship, LC & Cox, NAEffect of fructooligosaccharide on salmonella colonization of the chicken intestine. Poult Sci 1991 70, 2433–2438.Google Scholar
Cavanagh, D & Naqi, SAInfectious bronchitis. In Disease of Poultry, 10th ed., pp. 511–526 [Calnek, BWBarnes, JHBeard, CWMcDougald, LRSaif, YMeditors].Ames, IA: Iowa State University Press. 1997Google Scholar
Erickson, KL & Hubbard, NEProbiotic immunomodulation in health and disease. J Nutr 2000 130, Suppl., 403S–409S.CrossRefGoogle ScholarPubMed
Freter, RExperimental enteric Shigella and Vibrio infections in mice and guinea pigs. J Exp Med 1956 104, 411–418.CrossRefGoogle ScholarPubMed
Fuller, RProbiotics in man and animals. J Appl Bacteriol 1989 66, 365–378.Google Scholar
Gibson, GR & Roberfroid, MBDietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 1995 125, 1401–1412.CrossRefGoogle ScholarPubMed
Gibson, GR & Wang, XBifidogenic properties of different types of fructo-oligosaccharides. Food Microbiol 1994 11, 491–498.CrossRefGoogle Scholar
Gusils, C, Gonzalez, SN & Oliver, GSome probiotic properties of chicken lactobacilli. Can J Microbiol 1999 45, 981–987.CrossRefGoogle ScholarPubMed
Haller, D, Bode, C, Hammes, WP, Pfeifer, AMA, Schiffrin, EJ & Blum, SNon-pathogenic bacteria elicit a differential cytokine responses by intestinal epithelial cell/leukocyte co-cultures. Gut 2000 47, 79–87.CrossRefGoogle ScholarPubMed
Koenen, ME, Heres, L, Claassen, E & Boeresma, WJALactobacilli as probiotics in chickens feeds. Biosci Microflora 2002 21, 209–216.CrossRefGoogle Scholar
MacGillivray, PC, Finlay, HVL & Binns, TBUse of lactulose to create a preponderance of lactobacilli in the intestine of bottle-fed infants. Scott Med J 1959 4, 182–189.CrossRefGoogle ScholarPubMed
Matsuzaki, TModulating immune responses with probiotic bacteria. Immunol Cell Biol 2000 78, 67–73.CrossRefGoogle ScholarPubMed
Matsuzaki, T, Yokokura, T & Mutai, MAntitumor effect of intrapleural administration of Lactobacillus casei in mice. Cancer Immunol Immunother 1988 26, 209–214.CrossRefGoogle ScholarPubMed
Miller, CProtective action of the normal microflora against enteric infection: an experimental study in the mouse. Univ Minn Med Bull 1959 25, 272–279.Google ScholarPubMed
Ogawa, T, Asai, Y, Yasuda, H & Sakamoto, HOral immunoadjuvant activity of a new synbiotic, Lactobacillus casei subsp. casei in conjunction with dextran in BALB/c mice. Nutr Res 2005 25, 295–304.Google Scholar
Ogawa, T, Kotani, S & Shimauchi, HEnhancement of serum antibody production in mice by oral administration of lipophilic derivatives of muramyl peptides and bacterial lipopolysaccharides with bovine serum albumin. Methods Find Exp Clin Pharmacol 1986 8, 117–125.Google ScholarPubMed
Ogawa, T, Kusumoto, Y, Kiyono, H, McGhee, JR & Hamada, sOccurrence of antigen-specific B cells following oral or parenteral immunization with Porphyromonas gingivalis fimbriae. Int Immunol 1992 4, 1003–1010.CrossRefGoogle ScholarPubMed
Ogawa, T, Shimauchi, H, Furuta, R, Kawata, S, Yokogawa, K & Kotani, SOral immunoadjuvant activity of lipophilic derivatives of N-acetyl-glucosaminyl-b (1–4)-N-acetylmuramily-L-alanyl-Disoglutaminyl-(L)-meso-2,6-diaminopimelic acid-(D)-amide. Vaccine 1995 13, 887–889.Google Scholar
Ouwehand, AC, Salminen, S & Isolauri, EProbiotics: an overview of beneficial effects. Antonie Van Leeuwenhoek 2002 82, 279–289.CrossRefGoogle ScholarPubMed
Perdigón, G, de Macias, ME, Alvarez, s, Oliver, G & de Ruiz Holgado, AAEffect of perorally administered lactobacilli on macrophage activation in mice. Infect Immun 1986 53, 404–410.CrossRefGoogle ScholarPubMed
Rolfe, RDThe role of probiotics cultures in the control of gastrointestinal health. J Nutr 1959 130, Suppl., 396S–402S.CrossRefGoogle Scholar
Rowland, IR & Tanaka, RThe effects of transgalactosylated ligosaccharides on gut flora metabolism in rats associated with a human faecal microflora. J Appl Bacteriol 1993 74, 667–674.CrossRefGoogle ScholarPubMed
Sun, MUse of antibiotics in animal feed challenged. Science 1984 226, 144–146.Google Scholar
Takagi, A, Matsuzaki, T, Sato, M, Nomoto, K, Morotomi, M & Yokokura, TEnhancement of natural killer cytotoxicity delayed murine carcinogenesis by a probiotic microorganism. Carcinogenesis 2001 22, 599–605.CrossRefGoogle ScholarPubMed
van Den Bogaard, AE, Bruinsma, N & Stobberingh, EEThe effect of banning avoparcin on VRE carriage in the Netherlands. J Antimicrob Chemother 2000 46, 145–153.CrossRefGoogle ScholarPubMed
Villegas, PViral diseases of the respiratory system. Poult Sci 1998 77, 1143–1145.Google Scholar
Wang, C-H, Hong, C-C & Seak, JCHAn ELISA for antibodies against infectious bronchitis virus using an S1 spike polypeptide. Vet Microbiol 2002 85, 333–342.CrossRefGoogle ScholarPubMed
World Health Organization Impacts of Antimicrobial Growth Promoter Termination in Denmark. Geneva: WHO. 2003Google Scholar