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Oral administration of deuterium-labelled polyamines to sucking rat pups: luminal uptake, metabolic fate and effects on gastrointestinal maturation

Published online by Cambridge University Press:  09 March 2007

Bernard Dorhout ,
Anet Van Faassen ,
Christien M. Van Beusekom ,
Anneke W. Kingma ,
Elly De Hoog ,
Gijs T. Nagel ,
Arend Karrenbeld ,
E. Rudy Boersma  and
Frits A. J. Muskiet
Bernard Dorhout
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Anet Van Faassen
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Christien M. Van Beusekom
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Anneke W. Kingma
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Elly De Hoog
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Gijs T. Nagel
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Arend Karrenbeld
Affiliation:
Department of Pathology, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
E. Rudy Boersma
Affiliation:
Department of Obstetrics and Gynaecology, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
Frits A. J. Muskiet
Affiliation:
Central Laboratory for Clinical Chemistry, Nutrition & Development Unit, University and University Hospital Groningen, Oostersingel 59, PO Box 30.001, 9700 RB Groningen, The Netherlands
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Abstract

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Non-physiological amounts of oral polyamines have been reported to induce precocious gut maturation in rat pups. The aim of the present study was to investigate organ distribution and metabolic fate of orally administered stable-isotopically labelled polyamines in rat pups. Pups received tetradeuterium-labelled putrescine (Pu-d4; 3 μmol), spermidine (Sd-d4; 5 μmol), spermine (Sp-d4; 3 μmol), or physiological saline twice daily on postnatal days 7–10 or 12–15. They were killed on days 10 and 15. We determined activities of ileal lactase (EC 3.2.1.23), maltase (EC 3.2.1.20), sucrase (EC 3.2.1.48) and diamine oxidase (EC 1.4.3.6) and established villus and crypt lengths. Polyamines and their labelling percentages in organs were determined by GC and mass fragmentography. Treatments did not affect growth rate, but caused lower weights of liver, kidneys and heart. Maltase activity increased, lactase decreased, whereas sucrase and diamine oxidase did not change. Villus and crypt lengths increased. Organ polyamine pools were labelled to different extents. Irrespective of the orally administered polyamine, all organs contained Pu-d4, Sd-d4 and Sp-d4. Administered Pu-d4 and Sd-d4 were recovered mainly as Sd-d4, whereas Sp-d4 was recovered as Sp-d4 and Sd-d4. Total polyamines in a caecum, colon and erythrocytes increased, but increases were only to a minor extent with regard to labelled polyamines. Our data confirm precocious gut maturation by exogenous polyamines. Putrescine appears to be the limiting factor. The exogenous polyamines were distributed among all investigated organs. They are not only used for the synthesis of higher polyamines, but also retroconverted to their precursors. Changes in erythrocyte polyamine contents suggest precocious stimulation of erythropoiesis.

Keywords

PolyaminesGut maturationMetabolism
Type
General Nutrition
Information
British Journal of Nutrition , Volume 78 , Issue 4 , October 1997 , pp. 639 - 654
Copyright
Copyright © The Nutrition Society 1997

References

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