Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-23T16:21:20.614Z Has data issue: false hasContentIssue false
  • English
  • Français

Ontogeny of brush border carbohydrate digestion and uptake in the chick

Published online by Cambridge University Press:  09 March 2007

David Sklan ,
Asaf Geyra ,
Elad Tako ,
Orit Gal-Gerber  and
Zehava Uni
David Sklan*
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Asaf Geyra
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Elad Tako
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Orit Gal-Gerber
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
Zehava Uni
Affiliation:
Faculty of Agriculture, Hebrew University, PO Box 12, Rehovot, Israel
*
*Corresponding author: Professor D. Sklan, fax +972 8 9489865, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Ingestion of carbohydrates from the small intestine is the major route of energy supply in animals. In mammals these functions develop both pre- and postnatally and are coordinated for the sucking period. In birds, the physiological requirements are different and hatchlings ingest diets rich in complex carbohydrates soon after hatching. The present study examined the ontogeny of intestinal carbohydrate uptake in the chicken. The expression of mRNA for a brush border enzyme, sucrase–isomaltase (SI), which is critical in disaccharide digestion, was determined, together with that of the Na–glucose transporter (SGLT)-1, which is the major apical glucose transporter, In addition, the homeobox gene cdx, which is involved in inducing SI expression in mammals was examined. It was found that the expression of cdxA mRNA and cdxA protein increased from day 15 of incubation until hatch, after which further changes were small. CdxA protein was shown to bind to the promoter region of SI in the chick indicating that cdxA is similar to the mammalian cdx2. The mRNA of SI was observed at 15 d incubation, increased from 17 d of incubation to a peak on day 19, decreased at hatch and had a further peak of expression 2 d post-hatch. In contrast, the mRNA of SGLT-1 was not detected until 19 d of incubation when a major peak of expression was observed followed by a decrease to low levels at hatch and small increases post-hatch. It appears that both SI and SGLT-1 mRNA are expressed before hatch in the chick, but the ontogeny of expression is controlled by different mechanisms.

Keywords

Small intestineOntogenyCarbohydrateChick
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 6 , June 2003 , pp. 747 - 753
Copyright
Copyright © The Nutrition Society 2003

References

Boudreau, F, Zhu, Y & Traber, PG (2001) Sucrase–isomaltase gene transcription requires the hepatocyte nuclear factor-1 (HNF-1) regulatory element and is regulated by the ratio of HNF-1 alpha to HNF-1 beta. Journal of Biological Chemistry 276, 32122–32128.CrossRefGoogle ScholarPubMed
Chediack, JG, Caviedes-Vidal, E, Karasov, WH & Pestchanker, M (2001) Passive absorption of hydrophilic carbohydrate probes by the house sparrow Passer domesticus. Journal of Experimental Biology 204, 723–731.CrossRefGoogle ScholarPubMed
Chomczynski, P & Sacchi, N (1987) Single step method of RNA isolation by acid guanidium thiocyanate-phenol-choloroform extraction. Analytical Biochemistry 162, 156–159.Google Scholar
Devereux, J, Haeberli, P & Smithies, O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12, 387–395.CrossRefGoogle ScholarPubMed
Edelman, GM & Jones, FS (1993) Outside and downstream of the homeobox. Journal of Biological Chemistry 268, 20683–20686.Google Scholar
Fang, R, Santiago, NA, Olds, LC & Sibley, E (2000) The homeodomain protein Cdx2 regulates lactase gene promoter activity during enterocyte differentiation. Gastroenterology 118, 115–127.Google Scholar
Ferraris, RF & Diamond, J (1997) Regulation of intestinal sugar transport. Physiological Reviews 77, 257–302.Google Scholar
Ferraris, RP (2001) Dietary and developmental regulation of intestinal sugar transport. Biochemical Journal 360, 265–276.CrossRefGoogle ScholarPubMed
Frumkin, A, Pillemer, G, Haffner, R, Tarcic, N, Gruenbaum, Y & Fainsod, A (1994) A role for CdxA in gut closure and intestinal epithelia differentiation. Development 120, 253–263.CrossRefGoogle ScholarPubMed
Frumkin, A, Rangini, Z, Ben-Yehuda, A, Gruenbaum, Y & Fainsod, A (1991) A chicken caudal homologue, CHox-cad, is expressed in the epiblast with posterior localization and in the early endodermal lineage. Development 112, 207–219.CrossRefGoogle ScholarPubMed
Gal-Garber, O, Mabjeesh, SJ, Sklan, D & Uni, Z (2000) Partial sequence and expression of the gene for and activity of the sodium glucose transporter in the small intestine of fed, starved and refed chickens. Journal of Nutrition 130, 2174–2179.CrossRefGoogle ScholarPubMed
Geyra, A, Uni, Z, Gal-Gerber, O, Guy, D & Sklan, D (2002) Starving affects CDX gene expression during small intestinal development in the chick. Journal of Nutrition 132, 911–917.Google Scholar
Goda, T, Yasutake, H, Tanaka, T & Takase, S (1999) Lactase–phlorizin hydrolase and sucrase–isomaltase genes are expressed differently along the villus–crypt axis of rat jejunum. Journal of Nutrition 129, 1107–1113.Google Scholar
Huber, PA, Gao, Y, Fraser, ID, Copeland, O, El-Mezgoeldi, M, Slatter, DA, Keane, NE, Marston, SB & Levine, BA (1998) Structure-activity studies of the regulatory interaction of the 10 kilodalton C-terminal fragment of caldesmon with actin and the effect of mutation of caldesmon residues 691–696. Biochemistry 37, 2314–2326.CrossRefGoogle ScholarPubMed
Jiang, L & Ferraris, RP (2001) Developmental reprogramming of rat GLUT-5 requires de novo mRNA and protein synthesis. American Journal of Physiology 280, G113–G120.Google ScholarPubMed
Krasinski, SD, Van Wering, HM, Tannemaat, MR & Grand, RJ (2001) Differential activation of intestinal gene promoters: functional interactions between GATA-5 and HNF-1 alpha. American Journal of Physiology 281, G69–G84.Google ScholarPubMed
Lorentz, O, Duluc, I, De Arcangelis, A, Simon-Assman, P, Kedinger, M & Freund, JN (1997) Key role of the cdx2 homeobox gene in extracellular matrix-mediated intestinal cell differentiation. Journal of Cell Biology 139, 1553–1565.Google Scholar
Markowitz, AJ, Wu, GD, Bader, A, Cui, Z, Chen, L & Traber, PG (1995) Regulation of lineage-specific transcription of the sucrase–isomaltase gene in transgenic mice and cell lines. American Journal of Physiology 269, G925–G939.Google ScholarPubMed
Morales, AV, de la Rosa, EJ & de Pablo, F (1996) Expression of the cCdx-B homeobox gene in chick embryo suggests its participation in rostrocaudal axial patterning. Developmental Dynamics 206, 343–353.Google Scholar
National Research Council (1994) Nutrient Requirements of Poultry, 9th ed. Washington, DC: National Academy of Science.Google Scholar
Noy, Y & Sklan, D (2001) Yolk and exogenous feed utilization in the posthatch chick. Poultry Science 80, 1490–1495.Google Scholar
O'Connor, TP & Diamond, J (1999) Ontogeny of intestinal safety factors: lactase capacities and lactose loads. American Journal of Physiology 276, R753–R765.Google Scholar
Silberg, DG, Swain, GP, Suh, ER & Traber, PG (2000) Cdx1 and cdx2 expression during intestinal development. Gastroenterology 119, 961–971.Google Scholar
Sklan, D & Noy, Y (2000) Hydrolysis and absorption in the small intestines of posthatch chicks. Poultry Science 79, 1306–1310.Google Scholar
Suh, E, Chen, L, Taylor, J & Traber, PG (1994) A homeodomain protein related to caudal regulates intestine-specific gene transcription. Molecular and Cellular Biology 14, 7340–7351.Google Scholar
Sulistiyanto, B, Akiba, Y & Sato, K (1999) Energy utilisation of carbohydrate, fat and protein sources in newly hatched broiler chicks. British Poultry Science 40, 653–659.Google Scholar
Traber, PG (1997) Epithelial cell growth and differentiation. V. Transcriptional regulation, development, and neoplasia of the intestinal epithelium. American Journal of Physiology 273, G979–G981.Google ScholarPubMed
Tung, J, Markowitz, AJ, Silberg, DG & Traber, PG (1997) Developmental expression of SI is regulated in transgenic mice by an evolutionarily conserved promoter. American Journal of Physiology 273, G83–G92.Google Scholar
Uni, Z (1998) Research notes: Identification and isolation of chicken sucrase-isomaltase cDNA sequence. Poultry Science 77, 140–144.Google Scholar
Wu, GD, Chen, L, Forslund, K & Traber, PG (1994) Hepatocyte nuclear factor-1 alpha (HNF-1 alpha) and HNF-1 beta regulate transcription via two elements in an intestine-specific promoter. Journal of Biological Chemistry 269, 17080–17085.Google Scholar
Wu, GD, Wang, W & Traber, PG (1992) Isolation and characterization of the human sucrase–isomaltase gene and demonstration of intestine-specific transcriptional elements. Journal of Biological Chemistry 267, 7863–7870.Google Scholar