Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-13T09:19:54.583Z Has data issue: false hasContentIssue false
  • English
  • Français

Nutritional evaluation of sweet lupin (Lupinus angustifolius): net protein utilization (NPU), nitrogen balance and fractionation studies

Published online by Cambridge University Press:  09 March 2007

Mohammed H. Rahman ,
Iqbal Hossain  and
Moslehuddin
Mohammed H. Rahman
Affiliation:
Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh
Iqbal Hossain
Affiliation:
Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh
Moslehuddin
Affiliation:
Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effects of raw sweet lupin (Lupinus angustifolius) meal and its fractions on the growth and N utilization of rats were determined in two NPU and five N balance experiments. Sweet lupinseed grown in Western Australia, obtained as meal, unsupplemented (LMU), or fully supplemented with required amino acids (360 g/kg diet) (LMFS) was tested. In addition, six fractions were tested: aqueous non-dialysed extract at pH 7·0 (LPAND), dialysed extracts soluble (LPAD) and insoluble at pH 7·0 (LPADI), buffer-soluble extract at pH 7·0 (BUSOL), buffer-insoluble extract after dialysis at pH 7·0 (BUDI) and the residue (LMR) containing most of the material from meal insoluble in water and phosphate-citrate buffer. All diets based on fractions contained the same amounts of energy and protein and were supplemented with amino acids, vitamins and minerals to target requirements. Body N and lipid contents of rats fed on LMU and LMFS were reduced significantly in comparison with rats fed on positive lactalbumin (LACT) and non-protein diets (NPC) as negative controls. This wasdue in part to the lower retention of the absorbed N. As a result, the NPU and the biological value (BV) of sweet lupinseed proteins were less than expected. Urea-N outputs of the LMU- and LMFS-fed rats were also elevated. In contrast, true N and DM digestibilities of rats fed on LMU and LMFS were not significantly affected by the difference in the energy content of the diet. The replacement of lactalbumin in thediet with LPAND (196 g/kg), LPAD (148 g/kg), LPADI (124 g/kg), BUSOL (136 g/kg) or BUDI (119 g/kg) reduced dry body weight, N and lipid contents, NPU and BV compared with those obtained from the LACT control, even though the N and DM digestibilitieswere not significantly different. Inclusion of the residue fraction (170 g LMR/kg) had no apparent effect on any of the variables studied. Since sweet lupinseed had asmall amount of non-reactive lectin and LMR had some undesirable side-effects in these rats, it appears that the low nutritional value of LMFS for rats (NPU 0·62) despite the very high level of digestibility of its N, results from disturbances in N metabolism, and particularly from the low retention value of the absorbed N

Keywords

Lupinus angustifoliusNutritional effectsRats
Type
General Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 3 , March 1997 , pp. 443 - 457
Copyright
Copyright © The Nutrition Society 1997

References

REFERENCES

Åman, P. & Hesselman, K. (1984). Analysis of starch and other main constituents of cereal grains. Swedish Journal of Agricultural Research 14, 135–139.Google Scholar
Batterham, E. S. (1979). Lupinus albus cv. Ultra and Lupinus angustifolius cv. Unicrop as protein concentrates for growing pigs. Australian Journal of Agricultural Research 30, 369–375.CrossRefGoogle Scholar
Batterham, E. S., Andersen, L. M., Burnham, B. V. & Taylor, G. A. (1986). Effect of heat on the nutritional value of lupin (Lupinus angustifolius) seed meal for growing pigs. British Journal of Nutrition 55, 169–177.CrossRefGoogle ScholarPubMed
Coates, M. E., O'Donoghue, P. N., Payne, P. R. & Ward, R. J. (1969). Laboratory Animal Hand Books. 2. Dietary Standards for Laboratory Rats and Mice, p. 15. London: Laboratory Animals Ltd.Google Scholar
Dagnia, S. G., Petterson, D. S., Bell, R. R. & Flanagan, F. V. (1992). Germination alters the chemical composition and protein quality of lupin seeds. Journal of the Science of Food and Agriculture 60, 419–423.CrossRefGoogle Scholar
Davidson, J., Mathieson, J. & Boyne, A. W. (1970). The use of automation in determining nitrogen by the Kjeldahl method, with final calculations by computer. Analyst 95, 181–193.CrossRefGoogle ScholarPubMed
Egana, J. I., Uauy, R., Cassarola, X., Barrera, G. & Yanez, E. (1992). Sweet lupin protein quality in young men. Journal of Nutrition 122, 2341–2347.CrossRefGoogle ScholarPubMed
Englyst, H. N. & Cummings, J. H. (1984). Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars and alditol acetates. Analyst 109, 937–942.CrossRefGoogle Scholar
Evans, A. J., Cheung, P. C.-K. & Cheetham, W. H. (1993). The carbohydrate composition of cotyledons and hulls of Lupinus angustifolius from Western Australia. Journal of the Science of Food and Agriculture 63, 189–194.CrossRefGoogle Scholar
Grant, G., Moore, L. J., McKenzie, N. H., Stewart, J. C. & Pusztai, A. (1983). A survey of the nutritional and haemagglutination properties of legume seeds generally available in the UK. British Journal of Nutrition 50, 207–214.CrossRefGoogle ScholarPubMed
Kim, C. S. & Madhusudan, K. T. (1988). Haemagglutination and trypsin inhibitor activities of lupin seed (lupinus angustifolius). Journal of Food Science 53, 1234–1235.CrossRefGoogle Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
McIlvaine, T. C. (1921). A buffer solution for colorimetric comparison. Journal of Biological Chemistry 69, 183–186.CrossRefGoogle Scholar
Marquardt, R. R. (1983). Antimetabolites in faba beans: their metabolic significance. FABIS News Letter 7, 1–4.Google Scholar
Miller, D. S. & Bender, A. E. (1955). The determination of the net protein utilization of proteins by a shortened method. British Journal of Nutrition 9, 382–388.CrossRefGoogle ScholarPubMed
Moore, S. (1963). On the determination of cystine as cystic acid. Journal of Biological Chemistry 238, 235–237.CrossRefGoogle Scholar
Moore, S., Spackman, D. H. & Stein, W. H. (1958). Chromatography of amino acids on sulphonated polystyrene resins. Analytical Chemistry 30, 1185–1190.CrossRefGoogle Scholar
Mosse, J. (1990). Nitrogen to protein conversion factor for ten cereals and six legumes or oil seeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content. Journal of Agricultural and Food Chemistry 38, 18–24.CrossRefGoogle Scholar
Murphy, S. R., McNiven, M. A., MacLeod, J. A. & Halliday, L. J. (1993). Grass and lupin silage in rations for beef steers supplemented with barley or potatoes. Animal Feed Science and Technology 40, 273–283.CrossRefGoogle Scholar
Palmer, R., McIntosh, A. & Pusztai, A. (1973). The nutritional evaluation of kidney bean (Phuseolus vulgaris). The effect on nutritional value of seed germination and changes in trypsin inhibitor content. Journal of the Science of Food and Agriculture 24, 937–944.CrossRefGoogle ScholarPubMed
Petterson, D. S., Allen, D. G., Greirson, B. N., Hancock, G. R., Harris, D. J. & Legge, F. M. (1986). The suitability of Lupinus angustifolius seed for human consumption. Proceedings of the Nutrition Society of Australia 11, 118.Google Scholar
Pusztai, A. (1991). Plant Lectins, p. 28. Cambridge: Cambridge University Press.Google Scholar
Pusztai, A., Clarke, E. M. W., Grant, G. & King, T. P. (1981). The toxicity of Phaseolus vulgaris lectins. Nitrogen balance and immunochemical studies. Journal of the Science of Food and Agriculture 32, 1037–1046.CrossRefGoogle ScholarPubMed
Pusztai, A., Clarke, E. M. W. & King, T. P. (1979). The nutritional toxicity of Phaseolus vulgaris lectins. Proceedings of the Nutrition Society 38, 115–120.CrossRefGoogle ScholarPubMed
Rahman, M. H. (1994). Chemical and nutritional evaluation of Lupinus angustifolius L. (sweet lupin) seed proteins and its fractions on general metabolism of monogastric animals. PhD Thesis, University of Aberdeen.Google Scholar
Rahman, M. H. (1995 a). Protein quality of sweet lupin and its effects on certain haematobiochemical constituents in growing rats. Bangladesh Veterinary Journal 28, 57–62.Google Scholar
Rahman, M. H. (1995 b). Nutritional response of growing rats to sweet lupin (L. angustifolius) and sweet lupin fractions. In Proceedings of 2nd European Conference on Grain Legumes-1995, pp. 296–297. Copenhagen: AEP (European Association for Grain Legume Research).Google Scholar
Rahman, M. H., Hossain, M. I. & Moslehuddin, (1996 a). Faecal composition and nitrogen excretion of rats fed on diets containing sweet lupin (Lupinus angustifolius L.) or its fractions. Journal of the Science of Food and Agriculture 70, 468–474.3.0.CO;2-T>CrossRefGoogle Scholar
Rahman, M. H., Hossain, M. I. & Moslehuddin, (1996 b). Mineral balance of rats fed on diets containing sweet lupin (Lupinus angustifolius L.) or its fractions. Animal Feed Science and Technology (In the Press).3.0.CO;2-T>CrossRefGoogle Scholar
Rubio, L. A., Grant, G., Bardocz, S., Dewey, P. & Pusztai, A. (1992). Mineral excretion of rats fed on diets containing faba beans (Vicia faba L.) or faba bean fractions. British Journal of Nutrition 67, 295–302.CrossRefGoogle ScholarPubMed
Yen, P. Y., Grant, G., Fuller, M. F. & Pusztai, A. (1989). Nutritional evaluation of sweet lupinseed (Lupinus angustifolius). Proceedings of the Nutrition Society 49, 145A.Google Scholar