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Nitrogen and purine metabolism at varying energy and protein supplies in sheep sustained on intragastric infusion

Published online by Cambridge University Press:  09 March 2007

J. E. Lindberg  and
K.-G. Jacobsson
J. E. Lindberg
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Kungsägens gård, S-753 23 Uppsala, Sweden
K.-G. Jacobsson
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Kungsägens gård, S-753 23 Uppsala, Sweden
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Abstract

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Wether sheep were fitted with rumen fistulas and polyethylene tubes to the abomasum and were given all nutrients by intragastric infusion. In Expt 1 volatile fatty acids (VFA) were given at 340, 450 and 630 kJ gross energy (GE)/kg metabolic weight (W0.75) and protein at 0, 150, 300, 600, 900 and 1500 mg nitrogen/kg W0.75. In Expt 2 VFA were infused at 450 kJ GE/kg W0.75 and protein at 0 and 300 mg N/kg W0.75. At all levels of energy intake in Expt 1 the N retention was significantly (P < 0.01) related to N intake. The basal N requirement was estimated to be 281 mg (SE 21.8) N/kg W0.75 at 340 kJ VFA/kg W0.75, 226 (SE 21.8) mg N/kg W0.75 at 450 kJ VFA/kg W0.75 and 207 (SE 19.4) mg N/kg W0.75 at 630 kJ VFA/kg W0.75. Plasma urea concentrations varied markedly in relation to protein intake and to energy supply. On the other hand plasma ammonia, glucose, insulin and creatinine concentrations, and also urinary excretion of purine derivatives and creatinine were not significantly affected by the treatments imposed. It was concluded that the urinary excretion of purine derivatives in ruminants was largely unaffected by moderate changes in energy intake and by large changes in protein intake.

Keywords

Nitrogen metabolismPurine derivativesProtein and energy intakeSheep
Type
Nitrogen Metabolism
Information
British Journal of Nutrition , Volume 64 , Issue 2 , September 1990 , pp. 359 - 370
Copyright
Copyright © The Nutrition Society 1990

References

Batch, C. C. (1967). Problems in predicting the value of non-protein nitrogen as a substitute for protein in rations for farm animal ruminants. World Review of Animal Production 3, 84–91.Google Scholar
Dunn, O. J. & Clark, V. A. (1974). Applied Statistics: Analysis of Variance and Regression. New York: John Wiley & Sons.Google Scholar
Fattet, I., Hovell, F. D. Deb., Ørskov, E. R., Kyle, D. J., Pennie, K. & Smart, R. I. (1984). Undernutrition in sheep. The effect of supplementation with protein on protein accretion. British Journal of Nutrition 52, 561–574.CrossRefGoogle ScholarPubMed
Fujihara, T., Ørskov, E. R., Reeds, P. J. & Kyle, D. J. (1987). The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intra-gastric nutrition. Journal of Agricultural Science, Cambridge 109, 7–12.CrossRefGoogle Scholar
Giesecke, D., Stangassinger, M. & Tiemeyer, W. (1984). Nucleic acid digestion and urinary purine metabolites in sheep nourished by intragastric infusions. Canadian Journal of Animal Science 64 Suppl., 144–145.CrossRefGoogle Scholar
Hovell, F. D. Deb., Ørskov, E. R., Grubb, D. A. & MacLeod, N. A. (1983 a). Basal urinary nitrogen excretion and growth response to supplemental protein by lambs close to energy equilibrium. British Journal of Nutrition 50, 173–187.CrossRefGoogle ScholarPubMed
Hovell, F. D. Deb., Ørskov, E. R., MacLeod, N. A. & McDonald, I. (1983 b). The effect of changes in the amount of energy infused as volatile fatty acids on the nitrogen retention and creatinine excretion of lambs wholly nourished by intragastric infusion. British Journal of Nutrition 50, 331–343.CrossRefGoogle ScholarPubMed
Lindberg, J. E. (1985). Urinary allantoin excretion and digestible organic matter intake in dairy goats. Swedish Journal of Agricultural Research 15, 31–37.Google Scholar
Lindberg, J. E. (1989). Nitrogen metabolism and urinary excretion of purines in goat kids. British Journal of Nutrition 61, 309–321.CrossRefGoogle ScholarPubMed
Lindberg, J. E., Bristav, H. & Manyenga, A. R. (1989). Excretion of purines in the urine of sheep in relation to duodenal flow of microbial protein. Swedish Journal of Agricultural Research 19, 45–52.Google Scholar
Lindberg, J. E. & Jansson, C. (1989). A rapid automated analysis of allantoin in ruminant urine. Swedish Journal of Agricultural Research 19, 163–167.Google Scholar
McAllan, A. B. (1982). The fate of nucleic acids in ruminants. Proceedings of the Nutrition Society 41, 309–317.CrossRefGoogle ScholarPubMed
Øskov, E. R., Grubb, D. A., Smith, J. S., Webster, A. J. F. & Corrigal, W. (1979 a). Efficiency of utlization of volatile fatty acids for maintenance and energy retention in sheep. British Journal of Nutrition 41, 541–551.CrossRefGoogle Scholar
Ørskov, E. R., Grubb, D. A., Wenham, G. & Corrigal, W. (1979 b). The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acids and protein. British Journal of Nutrition 41, 553–558.CrossRefGoogle Scholar
Ørskov, E. R. & MacLeod, N. A. (1982). The determination of the minimal nitrogen excretion in steers and dairy cows and its physiological and practical implications. British Journal of Nutrition 47, 625–636.CrossRefGoogle ScholarPubMed
Ørskov, E. R., MacLeod, N. A., Fahmy, S. T. M., Istasse, L. & Hovell, F. D. Deb. (1983). Investigation on nitrogen balance in dairy cows and steers nourished by intragastric infusion. Effects of submaintenance energy input with or without protein. British Journal of Nutrition 50, 99–107.CrossRefGoogle ScholarPubMed
Rys, R., Antoniwicz, A. & Maciejewicz, J. (1975). Allantoin in urine as an index of microbial protein in the rumen. In Tracer Studies on Non-protein Nitrogen in Ruminants, vol. II, pp. 95–98. Vienna: International Atomic Energy Agency.Google Scholar
SAS (1982). SAS User's Guide: Statistics. Cary, NC: SAS Institute.Google Scholar
Sibanda, S. (1982). The excretion of allantoin and other urinary nitrogen compounds by ruminants in relation to microbial protein production and dietary energy and protein. MSc Thesis, University of Aberdeen, Scotland.Google Scholar
Storm, E., Ørskov, E. R. & Smart, R. (1983). The nutritive value of rumen micro-organisms in ruminants. 2. The apparent digestibility and net utilization of microbial nitrogen for growing lambs. British Journal of Nutrition 50, 471–478.CrossRefGoogle ScholarPubMed
Technicon Instruments Inc. (1972). Technicon Clinical Method no. 01. Basingstoke: Technicon Instruments Co. Ltd.Google Scholar
Technicon Instruments Inc. (1977 a). Technicon Industrial Method no. 334–74W/B+. Basingstoke: Technicon Instruments Co. Ltd.Google Scholar
Technicon Instruments Inc. (1977 b). Technicon Industrial Method no. SF4–011FC7. Basingstoke: Technicon Instrument Co. Ltd.Google Scholar
Walker, D. M. (1967). Nitrogen balance studies with the milk-fed lamb. 6. Effect of starvation and realimentation. British Journal of Nutrition 21, 289–308.CrossRefGoogle ScholarPubMed
Walker, D. M. & Faichney, G. J. (1964). Nitrogen balance studies with the milk-fed lamb. 2. The partition of urinary N and sulphur. British Journal of Nutrition 18, 201–207.CrossRefGoogle Scholar
Watts, R. W. E. (1980). Biochemical aspects of nucleic acid metabolism. In European Association of Animal Production, Publication no. 27, pp. 106–117. Braunschweig: Federal Republic of Germany.Google Scholar
Young, E. G. & Conway, C. F. (1942). On the estimation of allantoin by the Rimini-Schryver reaction. Journal of Biological Chemistry 142, 839–852.CrossRefGoogle Scholar