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Muscle protein synthesis in response to testosterone administration in wether lambs

Published online by Cambridge University Press:  09 March 2007

G. E. Lobley ,
Alexmary Connell ,
E. Milne ,
Vivien Buchan ,
A. G. Calder ,
Susan E. Anderson  and
Hazel Vint
G. E. Lobley
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Alexmary Connell
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
E. Milne
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Vivien Buchan
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
A. G. Calder
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Susan E. Anderson
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
Hazel Vint
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB
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Abstract

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A method has been developed based on stable isotopes and biopsy procedures which allows the large-dose procedure for measurement of protein synthesis to be applied in serial studies to farm species. Measurements of total nitrogen retention and protein synthesis in m. longissimus dorsi and m. vastus lateralis were made in five wether lambs (40–44 kg) infused intravenously, successively, with vehicle (10 d); testosterone (15 d; 9 mg/d); vehicle (15 d). N retention was improved by testosterone infusion (+2.9 g N/d; a 96% improvement total over control periods). Muscle protein synthesis was not significantly altered by exogenous hormone administration, nor were RNA:protein, RNA:DNA or protein: DNA. The implication of the developed procedure for dynamic studies in accessible tissues of large animals is discussed.

Keywords

Protein synthesisTestosteroneLambsMuscle
Type
Synthesis of Muscle Protein
Information
British Journal of Nutrition , Volume 64 , Issue 3 , November 1990 , pp. 691 - 704
Copyright
Copyright © The Nutrition Society 1990

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