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Monocyte chemoattractant protein-1 involvement in the α-tocopherol-induced reduction of atherosclerotic lesions in apolipoprotein E knockout mice

Published online by Cambridge University Press:  07 June 2007

Maria C. G. Peluzio ,
Emir Miguel Jr ,
Thaís C. Drumond ,
Giovana C. César ,
Helton C. Santiago ,
Mauro M. Teixeira ,
Enio C. Vieira ,
Rosa M. E. Arantes  and
Jacqueline I. Alvarez-Leite
Maria C. G. Peluzio
Affiliation:
Departamento de Nutrição e Saúde, Universidade Federal de Viçosa, Av PH Rolls s/n. 36570-000, Brazil
Emir Miguel Jr
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Thaís C. Drumond
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Giovana C. César
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Helton C. Santiago
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Mauro M. Teixeira
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Enio C. Vieira
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Rosa M. E. Arantes
Affiliation:
Departamento de Patologia Geral, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
Jacqueline I. Alvarez-Leite*
Affiliation:
Departamento de Bioquímica e Imunologia, ICB, Universidade Federal de Minas Gerais, Caixa Postal 486, 30 161-970, Belo Horizonte–MG, Brazil
*
*Corresponding author: Dr Jacqueline I. Alvarez-Leite, fax +55 31 441 5963, email [email protected]
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Abstract

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We studied the effects of α-tocopheryl acetate supplementation on the development of fatty streaks and its ability to modulate the expression of monocyte chemoattractant protein (MCP)-1 in aortic lesions of apoliprotein E knockout mice. For this purpose, 16-week-old apolipoprotein E knockout mice received α-tocopherol supplemention ((800 mg)/kg diet) for 6 weeks. After this time, total and lipoprotein cholesterol in the serum, hepatic tocopherol, aortic lesion area and MCP-1 (protein and mRNA) expression were analysed. Our present results showed that the dietary supplementation with α-tocopherol did not reduce serum cholesterol nor change lipoprotein profile, but it reduced the area of the aortic lesion by 55%. The reduction in the lesion size was correlated with the reduced expression of MCP-1 mRNA and protein, as detected by real-time quantitative polymerase chain reaction and immunohistochemistry respectively. In conclusion, the results obtained here are relevant to the study of atherosclerosis, as they correlate the effectiveness of vitamin E supplementation in inhibiting the plaque formation with diminished expression of MCP-1 at the aortic lesion.

Keywords

AtherosclerosisApolipoprotein E knockout miceα-TocopherolMonocyte chemoattractant protein-1Antioxidant
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 1 , July 2003 , pp. 3 - 11
Copyright
Copyright © The Nutrition Society 2003

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