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Modulation of the regression of atherosclerosis in the hamster by dietary lipids: comparison of coconut oil and olive oil

Published online by Cambridge University Press:  09 March 2007

E. Heather Mangiapane ,
Martina A. McAteer ,
G. Martin Benson ,
David A. White  and
Andrew M. Salter
E. Heather Mangiapane
Affiliation:
Division of Nutritional Biochemistry, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK
Martina A. McAteer
Affiliation:
Division of Nutritional Biochemistry, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK
G. Martin Benson
Affiliation:
Vascular Biology Department, SmithKline Beecham Pharmaceuticals Ltd, Harlow, Essex CM19 5AW, UK
David A. White
Affiliation:
School of Biomedical Sciences, University of Nottingham Medical School, Nottingham, NG7 2UH, UK
Andrew M. Salter*
Affiliation:
Division of Nutritional Biochemistry, School of Biological Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK
*
*Corresponding author: Dr Andrew Salter, fax +44 (0)115 9516122, email [email protected]
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Abstract

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The Golden Syrian hamster (Mesocricetus auratus) has been shown to be a useful model of both human lipoprotein metabolism and the development of atherosclerosis. We report the effects of dietary lipids on the progression and regression of atherosclerosis in this model. In the first study, hamsters fed on coconut oil (150 g/kg diet) and cholesterol (30 g/kg diet) developed lipid-rich lesions in the ascending aorta (0·28 (sd 0·14) mm2) and aortic arch (0·01 (sd 0·01) mm2) after 4 weeks that continued to progress over the next 8 weeks (0·75 (sd 0·41) mm2 and 0·12 (sd 0·11) mm2 for the ascending aorta and aortic arch respectively). Removal of cholesterol from the diet halted this progression. Furthermore, in animals fed on olive oil in the absence of added cholesterol, plasma LDL-cholesterol concentrations were lower (P < 0·05) and the extent of atherosclerotic lesions was reduced (P < 0·001 for both regions of the aorta) compared with animals fed on coconut oil (with no added cholesterol). In a second study, animals were fed on the atherogenic diet for 10 weeks, transferred to diets containing either coconut oil (150 g/kg diet) or olive oil (150 g/kg diet) without added cholesterol and monitored for up to 16 weeks. In the ascending aorta, lesion size doubled in animals fed on coconut oil but stabilized in those fed on olive oil. In the aortic arch, lesion size decreased linearly (P < 0·05, P < 0·001 for coconut oil and olive oil respectively) with the greatest reduction being seen in the olive-oil-fed animals (P < 0·05). Again, progression and regression of atherosclerosis appeared to reflect the relative concentrations of LDL-cholesterol and HDL-cholesterol in the plasma. We conclude that the male Golden Syrian hamster represents a useful model of dietary induced regression as well as progression of atherosclerosis.

Keywords

HamsterAtherosclerosisCoconut oilOlive oilDietary fat
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 5 , November 1999 , pp. 401 - 409
Copyright
Copyright © The Nutrition Society 1999

References

Bennett, AJ, Billett, MA, Salter, AM, Mangiapane, EH, Bruce, JS, Anderton, KL, Marenah, CB, Lawson, N & White, DA (1995) Modulation of hepatic lipoprotein B, 3-hydroxy-3-methyl-glutaryl-CoA reductase and low density lipoprotein receptor mRNA and plasma lipoprotein concentrations by defined dietary fats. Biochemical Journal 311, 167–173.CrossRefGoogle Scholar
Breslow, JL (1996) Mouse models of atherosclerosis. Science 272, 685–688.CrossRefGoogle ScholarPubMed
Foxall, TL, Shwaery, GT, Stucchi, AF, Nicolosi, RJ & Wong, SS (1992) Dose-related effects of Doxazosin on plasma lipids and aortic fatty streak formation in the hypercholesterolemic hamster model. American Journal of Pathology 140, 1357–1363.Google ScholarPubMed
Ishibashi, S, Goldstein, JL, Brown, MS, Herz, J & Burns, DK (1994) Massive xanthomatosis and atherosclerosis in cholesterol-fed low density lipoprotein receptor-negative mice. Journal of Clinical Investigation 93, 1885–1893.CrossRefGoogle ScholarPubMed
Kahlon, TS, Chow, FI, Irving, DW & Sayre, RN (1996) Cholesterol response and foam cell formation in hamsters fed two levels of saturated fat and various levels of cholesterol. Nutrition Research 16, 1353–1368.CrossRefGoogle Scholar
Kowala, MC, Nunnari, JJ, Durham, SK & Nicolosi, RJ (1991) Doxazosin and cholestyramine similarly decrease fatty streak formation in the aortic arch of hyperlidemic hamsters. Atherosclerosis 91, 35–49.CrossRefGoogle ScholarPubMed
Kowala, MC, Mazzucco, CE, Hartl, KS, Seiler, SM, Warr, GA, Abid, S & Grove, RI (1993) Prostacyclin agonists reduce early atherosclerosis in hyperlipidemic hamsters. Arteriosclerosis and Thrombosis 13, 435–444.CrossRefGoogle ScholarPubMed
Lindsey, S, Benattar, J, Pronczuk, A & Hayes, KC (1990) Dietary palmitic acid (C16: 0) enhanced HDL cholesterol and LDL receptor mRNA in hamsters. Proceedings of the Society for Experimental and Biological Medicine 195, 261–269.CrossRefGoogle Scholar
Mahler, VMG (1995) Coronary atherosclerosis stabilisation: an achievable goal. Atherosclerosis 118, S91–S101.CrossRefGoogle Scholar
Nakashima, Y, Plump, AS, Raines, EW & Breslow, JL (1994) ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arteriosclerosis and Thrombosis 14, 133–140.CrossRefGoogle ScholarPubMed
Nistor, A, Bulla, A, Filip, D & Radu, A (1987) The hyperlipidemic hamster as a model of experimental atherosclerosis. Atherosclerosis 68, 159–173.CrossRefGoogle Scholar
Oshima, R, Ikeda, T, Watanabe, K, Itakura, H & Sugiyama, N (1998) Probucol treatment attenuates the aortic atherosclerosis in Watanabe heritable hyperlipidemic rabbits. Atherosclerosis 137, 13–22.CrossRefGoogle ScholarPubMed
Otto, J, Ordovas, JM, Smith, D, van Dongen, D, Nicolosi, RJ & Schaefer, EJ (1995) Lovastatin inhibits diet induced atherosclerosis in F1B Golden Syrian hamsters. Atherosclerosis 114, 19–28.CrossRefGoogle ScholarPubMed
Padley, FB, Gunstone, FD & Harwood, JL (1994) Occurrence and characteristics of oils and fats. In The Lipid Handbook, pp. 47–223 [Gunstone, FB, Harwood, JL and Padley, FB, editors]. London: Chapman & Hall.Google Scholar
Parker, RA, Sabrah, T, Cap, M & Gill, BT (1995) Relation of vascular oxidative stress, α-tocopherol, and hypercholesterolemia to early atherosclerosis in hamsters. Arteriosclerosis, Thrombosis and Vascular Biology 15, 349–358.CrossRefGoogle ScholarPubMed
Pitman, WA, Osgood, DP, Smith, D, Schaefer, EJ & Ordovas, JM (1998) The effect of diet and lovastatin on regression of fatty streak lesions and on hepatic and intestinal mRNA levels for the LDL receptor and HMG CoA reductase in F1B hamsters. Atherosclerosis 138, 43–52.CrossRefGoogle ScholarPubMed
Rudel, LL, Parks, JS & Sawyer, JK (1995) Compared with dietary monounsaturated and saturated fat, polyunsaturated fat protects African Green monkeys from coronary-artery atherosclerosis. Arteriosclerosis, Thrombosis and Vascular Biology 15, 2101–2110.CrossRefGoogle ScholarPubMed
Salter, AM, Mangiapane, EH, Bennett, AJ, Bruce, JS, Bilett, MA, Anderton, KL, Marenah, CB, Lawson, N & White, DA (1998) The effect of different dietary fatty acids on lipoprotein metabolism: concentration-dependent effects of diets enriched in oleic, myristic, palmitic and stearic acids. British Journal of Nutrition 79, 195–202.CrossRefGoogle ScholarPubMed
Sessions, VA & Salter, AM (1994) The effects of different dietary fats and cholesterol on serum lipoprotein concentrations in hamsters. Biochimica et Biophysica Acta 1211, 207–214.CrossRefGoogle ScholarPubMed
Sima, A, Bulla, A & Simionescu, N (1990) Experimental obstructive atherosclerosis in the hyperlipidemic hamster. Journal of Submicroscopic and Cytologic Pathology 22, 1–16.Google ScholarPubMed
Spady, DK & Dietschy, JM (1988) Interaction of dietary cholesterol and triglycerides in the regulation of hepatic LDL transport in the hamster. Journal of Clinical Investigation 81, 300–309.CrossRefGoogle ScholarPubMed
Spady, DK, Woollett, LA & Dietschy, JM (1993) Regulation of plasma LDL-cholesterol by dietary cholesterol and fatty acids. Annual Reviews of Nutrition 13, 355–381.CrossRefGoogle ScholarPubMed
Stein, O, Dabach, Y, Hollander, G, Halperin, G, Thiery, J & Stein, Y (1996) Relative resistance of the hamster to aortic atherosclerosis in spite of prolonged vitamin E deficiency and dietary hypercholesterolemia. Putative effect of increased HDL? Biochimica et Biophysica Acta 1299, 216–222.CrossRefGoogle ScholarPubMed
Strong, JP, Bhattacharyya, AK, Eggen, DA, Malcom, GT, Newman, WP & Restrepo, C (1994) Long term induction and regression of diet induced atherosclerotic lesions in rhesus monkeys. 1. Morphological and chemical evidence for regression of lesions in the aorta and carotid and peripheral arteries. Arteriosclerosis and Thrombosis 14, 958–964.CrossRefGoogle ScholarPubMed
Vesselinovitch, D, Wissler, RW, Fischer-Dzoga, K, Hughes, R & DuBien, L (1974) Regression of atherosclerosis in rabbits 1. Treatment with low fat diet, hyperoxia and hypolipidemic agents. Atherosclerosis 19, 259–275.CrossRefGoogle Scholar
White, DA, Bennett, AJ, Billett, MA & Salter, AM (1997) Genetic determinants of plasma lipoprotein levels and their dietary response. Prostaglandins, Leukotrienes and Essential Fatty Acids 57, 455–462.CrossRefGoogle ScholarPubMed
Wissler, RW & Vesselinovitch, D (1990) Can atherosclerotic plaques regress? Anatomical and biochemical evidence from nonhuman animal models. American Journal of Cardiology 65, 33F–40F.CrossRefGoogle ScholarPubMed
Woollett, LA, Spady, DK & Dietschy, JM (1989) Mechanisms whereby saturated triacylglycerols elevate the plasma low density lipoprotein concentration in hamsters. Journal of Clinical Investigation 84, 119–128.CrossRefGoogle Scholar
Woollett, LA, Spady, DK & Dietschy, JM (1992) Regulatory effects of the saturated fatty acids 6:0 through 18:0 on hepatic low density lipoprotein receptor activity in the hamster. Journal of Clinical Investigation 89, 1133–1141.CrossRefGoogle Scholar
Zhu, B-Q, Sievers, RE, Isenberg, WM, Smith, DL & Parmley, WW (1990) Regression of atherosclerosis in cholesterol-fed rabbits: effects of fish oil and verapamil. Journal of the American College of Cardiology 15, 231–237.CrossRefGoogle ScholarPubMed
Zhu, B-Q, Sun, Y-P, Sievers, RE, Isenberg, WM, Moorhead, TJ & Parmley, WW (1994) Effects of etidronate and lovastatin on the regression of atherosclerosis in cholesterol-fed rabbits. Cardiology 85, 370–377.CrossRefGoogle ScholarPubMed